2021
Presynaptic Kv3 channels are required for fast and slow endocytosis of synaptic vesicles
Wu XS, Subramanian S, Zhang Y, Shi B, Xia J, Li T, Guo X, El-Hassar L, Szigeti-Buck K, Henao-Mejia J, Flavell RA, Horvath TL, Jonas EA, Kaczmarek LK, Wu LG. Presynaptic Kv3 channels are required for fast and slow endocytosis of synaptic vesicles. Neuron 2021, 109: 938-946.e5. PMID: 33508244, PMCID: PMC7979485, DOI: 10.1016/j.neuron.2021.01.006.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsCHO CellsCricetulusEndocytosisMiceMutationPresynaptic TerminalsShaw Potassium ChannelsSynaptic TransmissionSynaptic VesiclesConceptsSlow endocytosisVesicle mobilizationF-actin cytoskeletonChannel mutationsPotassium channelsKv3.3 proteinsInhibits endocytosisRapid endocytosisNovel functionF-actinEndocytosisCrucial functionSynaptic vesiclesFamily channelsSynaptic transmissionDiscovery decadesMembrane potentialNeurotransmitter releaseDiverse neurological disordersIon conductanceMutationsReleasable poolMouse nerve terminalsPotassium channel mutationsPathological effects
2010
Specific and rapid effects of acoustic stimulation on the tonotopic distribution of Kv3.1b potassium channels in the adult rat
Strumbos J, Polley D, Kaczmarek L. Specific and rapid effects of acoustic stimulation on the tonotopic distribution of Kv3.1b potassium channels in the adult rat. Neuroscience 2010, 167: 567-572. PMID: 20219640, PMCID: PMC2854512, DOI: 10.1016/j.neuroscience.2010.02.046.Peer-Reviewed Original ResearchMeSH KeywordsAcoustic StimulationAdaptation, PhysiologicalAnimalsAntibody SpecificityAuditory PathwaysAuditory ThresholdImmunohistochemistryIon Channel GatingNerve Tissue ProteinsNeuronal PlasticityRatsRats, Sprague-DawleyReaction TimeRhombencephalonShaw Potassium ChannelsSound LocalizationSynaptic TransmissionTime FactorsUp-RegulationConceptsTotal cellular levelsCytoplasmic C-terminusCellular levelVoltage-gated potassium channel subunitsPotassium channel subunitsTonotopic distributionAdult ratsC-terminusChannel proteinsChannel subunitsSound localization circuitIon channelsProteinExperience-dependent plasticityCultured neuronsPotassium channelsHigh-frequency stimuliAcute slicesMedial nucleusSynaptic activityAuditory neuronsKv3.1 proteinMin of exposureAction potentialsAcoustic stimulation
2008
Na+-mediated coupling between AMPA receptors and KNa channels shapes synaptic transmission
Nanou E, Kyriakatos A, Bhattacharjee A, Kaczmarek LK, Paratcha G, Manira A. Na+-mediated coupling between AMPA receptors and KNa channels shapes synaptic transmission. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 20941-20946. PMID: 19095801, PMCID: PMC2634910, DOI: 10.1073/pnas.0806403106.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsLampreysMembrane PotentialsNeuronsRatsReceptors, AMPASodium-Potassium-Chloride SymportersSynapsesSynaptic TransmissionRepetitive Firing Triggers Clustering of Kv2.1 Potassium Channels in Aplysia Neurons*
Zhang Y, McKay SE, Bewley B, Kaczmarek LK. Repetitive Firing Triggers Clustering of Kv2.1 Potassium Channels in Aplysia Neurons*. Journal Of Biological Chemistry 2008, 283: 10632-10641. PMID: 18276591, DOI: 10.1074/jbc.m800253200.Peer-Reviewed Original ResearchConceptsBag cell neuronsKv2.1 channelsPotassium channelsPlasma membraneC-terminusKv2.1 clustersKv2.1 potassium channelCell neuronsMammalian neuronsReproductive behaviorRectifier potassium channelFrequency-dependent broadeningRapid redistributionAplysia neuronsClamp recordingsAcid peptidePartial inactivationRing-like clustersPhysiological changesKv2.1Neuronal excitabilityCentral nervous systemAction potentialsGenesNervous system
2007
Bcl-xL Inhibitor ABT-737 Reveals a Dual Role for Bcl-xL in Synaptic Transmission
Hickman JA, Hardwick JM, Kaczmarek LK, Jonas EA. Bcl-xL Inhibitor ABT-737 Reveals a Dual Role for Bcl-xL in Synaptic Transmission. Journal Of Neurophysiology 2007, 99: 1515-1522. PMID: 18160428, PMCID: PMC2836590, DOI: 10.1152/jn.00598.2007.Peer-Reviewed Original ResearchConceptsMitochondrial outer membraneEndogenous Bcl-xLMitochondrial channel activityBcl-xLInhibitor ABT-737ABT-737Outer membraneBcl-xL.Pro-apoptotic cleavage productRecombinant Bcl-xLChannel activityBcl-xL proteinSynaptic functionDual roleGenetic toolsDomain pocketSynaptic transmissionSynaptic activityGiant presynaptic terminalEquivalent modificationEndogenous proteolysisRepetitive synaptic activityBH3Cleavage productsProtein
2005
Actions of BAX on Mitochondrial Channel Activity and on Synaptic Transmission
Jonas EA, Hardwick JM, Kaczmarek LK. Actions of BAX on Mitochondrial Channel Activity and on Synaptic Transmission. Antioxidants & Redox Signaling 2005, 7: 1092-1100. PMID: 16115013, DOI: 10.1089/ars.2005.7.1092.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBcl-2-Associated X ProteinBcl-X ProteinCell MembraneElectrophysiologyIntracellular MembranesLiposomesLoligoMitochondriaMultigene FamilyNeurotransmitter AgentsPatch-Clamp TechniquesPeptidesPresynaptic TerminalsProtein Structure, TertiarySynapsesSynaptic TransmissionTime FactorsConceptsMitochondrial membraneBcl-2 family proteins BaxCell deathOuter mitochondrial membraneAction of BaxMitochondrial channel activityChannel activityNormal physiological settingsAntiapoptotic Bcl-xL proteinBcl-xL proteinDeath channelMitochondrial architectureMitochondrial channelsProapoptotic fragmentsLarge conductance channelPresynaptic terminalsBcl-xL.Proapoptotic proteinsAlternative functionsProtein BaxPhysiological settingsPhysiological roleSynaptic transmissionBaxNeurotransmitter release
2003
Modulation of Synaptic Transmission by the BCL-2 Family Protein BCL-xL
Jonas EA, Hoit D, Hickman JA, Brandt TA, Polster BM, Fannjiang Y, McCarthy E, Montanez MK, Hardwick JM, Kaczmarek LK. Modulation of Synaptic Transmission by the BCL-2 Family Protein BCL-xL. Journal Of Neuroscience 2003, 23: 8423-8431. PMID: 12968005, PMCID: PMC6740692, DOI: 10.1523/jneurosci.23-23-08423.2003.Peer-Reviewed Original ResearchConceptsBcl-2 family proteinsProtein Bcl-xLBcl-xLFamily proteinsMitochondrial membranePro-apoptotic cleavage productRecombinant Bcl-xLBcl-xL proteinMitochondrial calcium uptakePresynaptic terminalsInfluences synaptic transmissionCell deathGiant presynaptic terminalSynaptic transmissionChannel activityProteinSquid stellate ganglionMitochondriaCleavage productsSynaptic stabilityAdult brainPostsynaptic responsesCalcium uptakeMembranePatch pipetteBAK Alters Neuronal Excitability and Can Switch from Anti- to Pro-Death Function during Postnatal Development
Fannjiang Y, Kim CH, Huganir RL, Zou S, Lindsten T, Thompson CB, Mito T, Traystman RJ, Larsen T, Griffin DE, Mandir AS, Dawson TM, Dike S, Sappington AL, Kerr DA, Jonas EA, Kaczmarek LK, Hardwick JM. BAK Alters Neuronal Excitability and Can Switch from Anti- to Pro-Death Function during Postnatal Development. Developmental Cell 2003, 4: 575-585. PMID: 12689595, DOI: 10.1016/s1534-5807(03)00091-1.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnimalsAnimals, NewbornApoptosisBcl-2 Homologous Antagonist-Killer ProteinCentral Nervous SystemCentral Nervous System DiseasesCentral Nervous System Viral DiseasesDisease Models, AnimalEpilepsyExcitatory Postsynaptic PotentialsGenetic VectorsHippocampusKainic AcidMaleMembrane ProteinsMiceMice, KnockoutNeurodegenerative DiseasesNeuronsNeurotoxinsProtein Structure, TertiarySindbis VirusStrokeSynaptic TransmissionConceptsNeuronal excitabilityVirus infectionPostnatal developmentAlters neuronal excitabilityKainate-induced seizuresSpinal cord neuronsIschemia/strokeSindbis virus infectionNeuronal injuryCord neuronsNeuronal deathProtective effectSynaptic activityMouse modelParkinson's diseaseNeuron subtypesNeurotransmitter releasePro-death functionMiceNeuronsSpecific death stimuliDeathSeizuresPossible roleExcitability
2001
Presynaptic target of Ca2+ action on neuropeptide and acetylcholine release in Aplysia californica
Ohnuma K, Whim M, Fetter R, Kaczmarek L, Zucker R. Presynaptic target of Ca2+ action on neuropeptide and acetylcholine release in Aplysia californica. The Journal Of Physiology 2001, 535: 647-662. PMID: 11559764, PMCID: PMC2278817, DOI: 10.1111/j.1469-7793.2001.00647.x.Peer-Reviewed Original ResearchConceptsSensory neuronsCholinergic synapsesPeptidergic synapsesAcetylcholine releasePostsynaptic responsesAction potentialsMost synaptic contactsIntracellular calcium concentrationPeptidergic vesiclesSingle action potentialAplysia californicaDose-response curveDense-core vesiclesNeuron B2Neuron B3Cholinergic transmissionSynaptic contactsLinear dose-response curvePostsynaptic neuronsPresynaptic neuronsCholinergic releasePresynaptic targetCholinergic vesiclesReleasable poolPatch pipetteTargeted Attenuation of Electrical Activity in Drosophila Using a Genetically Modified K+ Channel
White B, Osterwalder T, Yoon K, Joiner W, Whim M, Kaczmarek L, Keshishian H. Targeted Attenuation of Electrical Activity in Drosophila Using a Genetically Modified K+ Channel. Neuron 2001, 31: 699-711. PMID: 11567611, DOI: 10.1016/s0896-6273(01)00415-9.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalAnimalsBehavior, AnimalCells, CulturedDrosophila melanogasterDrosophila ProteinsFemaleGene DosageGene Expression Regulation, DevelopmentalGene TargetingGenes, LethalLarvaMembrane PotentialsMusclesMutationNervous SystemNeural InhibitionNeuronsNeurons, AfferentPhenotypePhotoreceptor Cells, InvertebratePotassium ChannelsShaker Superfamily of Potassium ChannelsSynaptic TransmissionTransgenes
1999
Prolonged Activation of Mitochondrial Conductances During Synaptic Transmission
Jonas E, Buchanan J, Kaczmarek L. Prolonged Activation of Mitochondrial Conductances During Synaptic Transmission. Science 1999, 286: 1347-1350. PMID: 10558987, DOI: 10.1126/science.286.5443.1347.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsCalciumCalcium ChannelsDecapodiformesElectric ConductivityElectric StimulationIntracellular MembranesIon ChannelsIon TransportMicroscopy, ElectronMitochondriaPatch-Clamp TechniquesPorinsPresynaptic TerminalsSynaptic TransmissionTime FactorsVoltage-Dependent Anion ChannelsConceptsChannel activityIon channel activityMitochondrial membraneOnly organellesIntracellular organellesIntact cellsIon channelsMitochondriaOrganellesLarge conductanceTens of secondsPresynaptic terminalsIon transportSynaptic transmissionSynaptic stimulationConductanceElectron microscopyPatch-clamp techniqueMembraneActivityCellsActivationSquidStimulation
1998
High-frequency firing helps replenish the readily releasable pool of synaptic vesicles
Wang L, Kaczmarek L. High-frequency firing helps replenish the readily releasable pool of synaptic vesicles. Nature 1998, 394: 384-388. PMID: 9690475, DOI: 10.1038/28645.Peer-Reviewed Original ResearchConceptsReleasable poolPotassium channel blocker tetraethylammoniumChannel blocker Cd2Synaptic vesiclesPresynaptic action potentialHigh-frequency stimulationVoltage-gated Ca2Short-term synaptic depressionCentral nervous systemPatch-clamp recordingsHigh-frequency firingGiant synapsesPostsynaptic mechanismsBuffer EGTAMouse auditoryBlocker tetraethylammoniumSynaptic activitySynaptic depressionPresynaptic terminalsNervous systemAction potentialsRate of replenishmentSynapsesCa2Key signal
1994
Transient changes in intracellular calcium associated with a prolonged increase in excitability in neurons of Aplysia californica
Fisher T, Levy S, Kaczmarek L. Transient changes in intracellular calcium associated with a prolonged increase in excitability in neurons of Aplysia californica. Journal Of Neurophysiology 1994, 71: 1254-1257. PMID: 8201416, DOI: 10.1152/jn.1994.71.3.1254.Peer-Reviewed Original ResearchConceptsIntracellular calcium levelsBag cell neuronsCalcium levelsSpontaneous firingCell neuronsIntracellular calciumAction potentialsTransient elevationBasal intracellular calcium levelsLong-duration action potentialsBasal calcium levelsFree calcium ion concentrationTriggers calcium releaseCalcium-sensitive microelectrodesAplysia californicaIndividual action potentialsAfferent stimulationAfferent inputAfterdischargesProlonged increaseCalcium releaseCalcium ion concentrationNeuronsBiphasic patternPrimary cultures
1982
An early sodium and a late calcium phase in the afterdischarge of peptide-secreting neurons ofAplysia
Kaczmarek L, Jennings K, Strumwasser F. An early sodium and a late calcium phase in the afterdischarge of peptide-secreting neurons ofAplysia. Brain Research 1982, 238: 105-115. PMID: 6282390, DOI: 10.1016/0006-8993(82)90774-0.Peer-Reviewed Original ResearchProtein phosphorylation during afterdischarge in peptidergic neurons of Aplysia
Jennings K, Kaczmarek L, Hewick R, Dreyer W, Strumwasser F. Protein phosphorylation during afterdischarge in peptidergic neurons of Aplysia. Journal Of Neuroscience 1982, 2: 158-168. PMID: 6278103, PMCID: PMC6564306, DOI: 10.1523/jneurosci.02-02-00158.1982.Peer-Reviewed Original Research
1981
The expression of long lasting afterdischarge by isolated Aplysia bag cell neurons
Kaczmarek L, Strumwasser F. The expression of long lasting afterdischarge by isolated Aplysia bag cell neurons. Journal Of Neuroscience 1981, 1: 626-634. PMID: 6286900, PMCID: PMC6564181, DOI: 10.1523/jneurosci.01-06-00626.1981.Peer-Reviewed Original Research