2024
Absence of the dolichol synthesis gene DHRSX leads to N-glycosylation defects in Lec5 and Lec9 Chinese hamster ovary cells
Kentache T, Althoff C, Caligiore F, Souche E, Schulz C, Graff J, Pieters E, Stanley P, Contessa J, Van Schaftingen E, Matthijs G, Foulquier F, Bommer G, Wilson M. Absence of the dolichol synthesis gene DHRSX leads to N-glycosylation defects in Lec5 and Lec9 Chinese hamster ovary cells. Journal Of Biological Chemistry 2024, 300: 107875. PMID: 39395802, DOI: 10.1016/j.jbc.2024.107875.Peer-Reviewed Original ResearchLec9 cellsChinese hamster ovaryDolichol synthesisGlycosylation-deficient Chinese hamster ovaryLong-read whole-genome sequencingCell linesN-glycosylation machineryN-glycan synthesisN-glycosylation defectChinese hamster ovary cellsGenomic regionsDHRSXLec9Hamster ovary cellsGlycosylation defectsHuman enzymeMolecular causesSynthesis defectsDolicholSRD5A3Hamster ovaryLevels of dolicholOvary cellsPolyprenolsKinetic properties
2021
The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress
Phoomak C, Cui W, Hayman TJ, Yu SH, Zhao P, Wells L, Steet R, Contessa JN. The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress. Science Advances 2021, 7: eabc6364. PMID: 33523898, PMCID: PMC7810369, DOI: 10.1126/sciadv.abc6364.Peer-Reviewed Original ResearchTranslocon-associated proteinN-glycosylationER stressER glycoprotein quality controlTranslocon-associated protein complexEndoplasmic reticulum (ER) homeostasisAberrant N-glycosylationGlycoprotein quality controlER chaperone BiPFluorescence-based strategyProtein complexesPosttranslational modificationsChaperone BiPTRAP complexGlycosylation defectsConditions of stressRegulatory roleTranscriptional signatureIndividual cellsDownstream ERProtein levelsSubunitsSSR3GlycosylationCells