2001
A Lithium-induced Conformational Change in Serotonin Transporter Alters Cocaine Binding, Ion Conductance, and Reactivity of Cys-109*
Ni Y, Chen J, Androutsellis-Theotokis A, Huang C, Moczydlowski E, Rudnick G. A Lithium-induced Conformational Change in Serotonin Transporter Alters Cocaine Binding, Ion Conductance, and Reactivity of Cys-109*. Journal Of Biological Chemistry 2001, 276: 30942-30947. PMID: 11408487, DOI: 10.1074/jbc.m104653200.Peer-Reviewed Original Research
2000
Functional Role of Critical Stripe Residues in Transmembrane Span 7 of the Serotonin Transporter EFFECTS OF Na+, Li+, AND METHANETHIOSULFONATE REAGENTS*
Kamdar G, Penado K, Rudnick G, Stephan M. Functional Role of Critical Stripe Residues in Transmembrane Span 7 of the Serotonin Transporter EFFECTS OF Na+, Li+, AND METHANETHIOSULFONATE REAGENTS*. Journal Of Biological Chemistry 2000, 276: 4038-4045. PMID: 11058600, DOI: 10.1074/jbc.m008483200.Peer-Reviewed Original ResearchConceptsCys-109Methanethiosulfonate reagentsWater-filled poresNative cysteine residuesCysteine-containing mutantsExtracellular loop 1Close-contact regionThree-dimensional structureCysteine residuesTranslocation mechanismControl mutantsAlpha-helixMTSEA-biotinResidue positionsCysteine substitutionsLoop 1Conformational changesMTS reagentsFunctional roleMutantsIon bindingResiduesTransportersIon dependenceSerotonin transporter
1992
p-Chloroamphetamine induces serotonin release through serotonin transporters.
Rudnick G, Wall S. p-Chloroamphetamine induces serotonin release through serotonin transporters. Biochemistry 1992, 31: 6710-8. PMID: 1322169, DOI: 10.1021/bi00144a010.Peer-Reviewed Original ResearchMeSH KeywordsBinding, CompetitiveBiological TransportBlood PlateletsCarrier ProteinsCell MembraneChloridesHumansHydrogen-Ion ConcentrationImipramineKineticsLithiumLithium ChlorideMembrane GlycoproteinsMembrane Transport ProteinsNerve Tissue ProteinsP-ChloroamphetamineRadioisotope Dilution TechniqueSerotoninSerotonin Plasma Membrane Transport ProteinsTritiumConceptsPlasma membrane vesiclesMembrane vesiclesChromaffin granule membrane vesiclesVesicular amine transporterATP hydrolysisBovine adrenal chromaffin granulesSerotonin transporterAdrenal chromaffin granulesPrevents accumulationAmine transportersPresence of Mg2TransportersVesiclesChromaffin granulesModel systemDelta pHHuman plateletsATPEffluxManner characteristicAccumulationTransmembraneNaCl gradientPCA's abilityP-chloroamphetamine