2001
The NH2-terminus of Norepinephrine Transporter Contains a Basolateral Localization Signal for Epithelial Cells
Gu H, Wu X, Giros B, Caron M, Caplan M, Rudnick G. The NH2-terminus of Norepinephrine Transporter Contains a Basolateral Localization Signal for Epithelial Cells. Molecular Biology Of The Cell 2001, 12: 3797-3807. PMID: 11739781, PMCID: PMC60756, DOI: 10.1091/mbc.12.12.3797.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCell LineCell PolarityDogsDopamine Plasma Membrane Transport ProteinsEpithelial CellsHumansMembrane GlycoproteinsMembrane Transport ProteinsMiceMicroscopy, ConfocalMolecular Sequence DataMutagenesis, Site-DirectedNerve Tissue ProteinsNorepinephrine Plasma Membrane Transport ProteinsProtein Sorting SignalsSequence AlignmentSymportersConceptsBasolateral localization signalLocalization signalDileucine motifPlasma membraneBasolateral localizationOverall amino acid sequence identityAmino acid sequence identityTerminal regionMDCK cellsApical plasma membraneBasolateral membraneEpithelial cellsSequence identityApical localizationChimeric proteinTransport assaysTransporter localizationAmino acidsApical membraneNorepinephrine transporterTransportersCorresponding sequenceDopamine transporterSame mutationMembrane
2000
Permeation and gating residues in serotonin transporter
Chen J, Rudnick G. Permeation and gating residues in serotonin transporter. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 1044-1049. PMID: 10655481, PMCID: PMC15515, DOI: 10.1073/pnas.97.3.1044.Peer-Reviewed Original ResearchMeSH KeywordsAllosteric RegulationAmino Acid SubstitutionCarrier ProteinsChloridesCocaineHumansHydrogenIon Channel GatingIon TransportIsoleucineMembrane GlycoproteinsMembrane Transport ProteinsMesylatesMutagenesis, Site-DirectedNerve Tissue ProteinsNorepinephrine Plasma Membrane Transport ProteinsOxidation-ReductionPotassiumProtein BindingProtein ConformationProtein Structure, TertiaryRecombinant Fusion ProteinsReducing AgentsSerotoninSerotonin Plasma Membrane Transport ProteinsSodiumSulfhydryl CompoundsSymportersConceptsSubstrate permeation pathway
1996
Cell-specific Sorting of Biogenic Amine Transporters Expressed in Epithelial Cells*
Gu H, Ahn J, Caplan M, Blakely R, Levey A, Rudnick G. Cell-specific Sorting of Biogenic Amine Transporters Expressed in Epithelial Cells*. Journal Of Biological Chemistry 1996, 271: 18100-18106. PMID: 8663573, DOI: 10.1074/jbc.271.30.18100.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiogenic AminesBiological TransportCarrier ProteinsCell CompartmentationCell MembraneCell PolarityCells, CulturedDogsDopamineDopamine Plasma Membrane Transport ProteinsEpithelial CellsHumansImmunohistochemistryMembrane GlycoproteinsMembrane Transport ProteinsNerve Tissue ProteinsNorepinephrineNorepinephrine Plasma Membrane Transport ProteinsRatsRecombinant ProteinsSerotoninSerotonin Plasma Membrane Transport ProteinsSymportersConceptsMadin-Darby canine kidneyMDCK cellsLLC-PK1 cellsNeurotransmitter transportersCell surface biotinylationConfocal immunofluorescence microscopyBasolateral membraneCell-specific mechanismsEpithelial cellsBiogenic amine transportersMembrane proteinsSurface biotinylationCDNA encodingHuman DA transporterAmine transportersImmunofluorescence microscopyBiotinylating reagentTransportersPermeable filter supportsApical surfaceImmunocytochemistry resultsBasolateral mediumSurface expressionApical sideDA transporterIon Coupling Stoichiometry for the Norepinephrine Transporter in Membrane Vesicles from Stably Transfected Cells (∗)
Gu H, Wall S, Rudnick G. Ion Coupling Stoichiometry for the Norepinephrine Transporter in Membrane Vesicles from Stably Transfected Cells (∗). Journal Of Biological Chemistry 1996, 271: 6911-6916. PMID: 8636118, DOI: 10.1074/jbc.271.12.6911.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesCarrier ProteinsCell LineCell MembraneChloridesIon TransportIonsNorepinephrineNorepinephrine Plasma Membrane Transport ProteinsPotassiumSodiumSwineSymportersTransfectionConceptsMembrane vesiclesTransmembrane ion gradientsLLC-PK1 cellsMajor substrateTransport substratesTransfected CellsStably Transfected CellsIon gradientsCoupling stoichiometryNet positive chargeDA accumulationVesiclesSubstrate moleculesTransportersNorepinephrine transporterAccumulationCellsGamma-aminobutyric acidCotransportDAGradientStoichiometrySubstrateAbsenceTransport processes
1995
Biogenic amine flux mediated by cloned transporters stably expressed in cultured cell lines: amphetamine specificity for inhibition and efflux.
Wall S, Gu H, Rudnick G. Biogenic amine flux mediated by cloned transporters stably expressed in cultured cell lines: amphetamine specificity for inhibition and efflux. Molecular Pharmacology 1995, 47: 544-50. PMID: 7700252.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-4-phenylpyridiniumAmphetaminesBiogenic MonoaminesBiological TransportCarrier ProteinsCell MembraneCells, CulturedCloning, MolecularCocaineDNA, ComplementaryDopamineDopamine Plasma Membrane Transport ProteinsHumansMazindolMembrane GlycoproteinsMembrane Transport ProteinsNerve Tissue ProteinsNeurotransmitter Uptake InhibitorsNorepinephrineNorepinephrine Plasma Membrane Transport ProteinsSerotoninSerotonin Plasma Membrane Transport ProteinsStimulation, ChemicalSubstrate SpecificitySymportersTransfectionConceptsBiogenic amine transportersCell linesAmine transportersRat serotonin transporterCultured cell linesInhibitor of transportRat dopamine transporterHuman norepinephrine transporterPlasma membraneLLC-PK1 cellsSubstrate effluxSubstrate influxDopamine transporterNorepinephrine transporterAmphetamine derivativesTransportersSerotonin transporterEffluxDistinct patternsP-chloroamphetamineAmine substratesCellsInhibited transportCDNAInhibitors
1994
Human norepinephrine transporter. Biosynthetic studies using a site-directed polyclonal antibody.
Melikian H, McDonald J, Gu H, Rudnick G, Moore K, Blakely R. Human norepinephrine transporter. Biosynthetic studies using a site-directed polyclonal antibody. Journal Of Biological Chemistry 1994, 269: 12290-12297. PMID: 8163533, DOI: 10.1016/s0021-9258(17)32714-x.Peer-Reviewed Original ResearchAnimalsAntibodiesCarrier ProteinsCell LineCysteineElectrophoresis, Polyacrylamide GelEnzyme-Linked Immunosorbent AssayFluorescent Antibody TechniqueHeLa CellsHumansImmunoblottingKineticsMethionineMolecular WeightNorepinephrineNorepinephrine Plasma Membrane Transport ProteinsRabbitsSulfur RadioisotopesSymportersTransfectionTunicamycinStable expression of biogenic amine transporters reveals differences in inhibitor sensitivity, kinetics, and ion dependence.
Gu H, Wall S, Rudnick G. Stable expression of biogenic amine transporters reveals differences in inhibitor sensitivity, kinetics, and ion dependence. Journal Of Biological Chemistry 1994, 269: 7124-7130. PMID: 8125921, DOI: 10.1016/s0021-9258(17)37256-3.Peer-Reviewed Original ResearchAnimalsBinding SitesBiogenic AminesBiological TransportCarrier ProteinsCell LineDopamine Plasma Membrane Transport ProteinsHumansKineticsMembrane GlycoproteinsMembrane Transport ProteinsNerve Tissue ProteinsNorepinephrine Plasma Membrane Transport ProteinsRatsRecombinant ProteinsSerotonin Plasma Membrane Transport ProteinsSymportersTransfection