1994
Ligand binding to the serotonin transporter: equilibria, kinetics, and ion dependence.
Humphreys C, Wall S, Rudnick G. Ligand binding to the serotonin transporter: equilibria, kinetics, and ion dependence. Biochemistry 1994, 33: 9118-25. PMID: 8049215, DOI: 10.1021/bi00197a014.Peer-Reviewed Original Research
1993
Amphetamine derivatives interact with both plasma membrane and secretory vesicle biogenic amine transporters.
Schuldiner S, Steiner-Mordoch S, Yelin R, Wall S, Rudnick G. Amphetamine derivatives interact with both plasma membrane and secretory vesicle biogenic amine transporters. Molecular Pharmacology 1993, 44: 1227-31. PMID: 7903417.Peer-Reviewed Original ResearchMeSH Keywords3,4-MethylenedioxyamphetamineAnimalsBlood PlateletsCarrier ProteinsCattleCell MembraneChromaffin GranulesFenfluramineHumansIn Vitro TechniquesMembrane GlycoproteinsMembrane Transport ProteinsNerve Tissue ProteinsN-Methyl-3,4-methylenedioxyamphetamineP-ChloroamphetamineReserpineSerotoninSerotonin Plasma Membrane Transport ProteinsConceptsVesicular amine transporterVesicular amine transportAmine transportersAmine transportSerotonin transportChromaffin granule membrane vesiclesPlasma membrane transportersBiogenic amine transportersPlasma membraneMembrane transportersMembrane vesiclesSubstrate siteVesicular transportersTransportersDelta pHBinding of the cocaine analog 2 beta-carbomethoxy-3 beta-(4-[125I]iodophenyl)tropane to serotonin and dopamine transporters: different ionic requirements for substrate and 2 beta-carbomethoxy-3 beta-(4-[125I]iodophenyl)tropane binding.
Wall S, Innis R, Rudnick G. Binding of the cocaine analog 2 beta-carbomethoxy-3 beta-(4-[125I]iodophenyl)tropane to serotonin and dopamine transporters: different ionic requirements for substrate and 2 beta-carbomethoxy-3 beta-(4-[125I]iodophenyl)tropane binding. Molecular Pharmacology 1993, 43: 264-70. PMID: 8429827.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding, CompetitiveBlood PlateletsCarrier ProteinsCocaineCorpus StriatumDopamineDopamine Plasma Membrane Transport ProteinsHydrogen-Ion ConcentrationIn Vitro TechniquesIonsMembrane GlycoproteinsMembrane Transport ProteinsNerve Tissue ProteinsOsmolar ConcentrationProtein BindingRadioligand AssayRatsSerotoninSerotonin Plasma Membrane Transport ProteinsNon-neurotoxic amphetamine derivatives release serotonin through serotonin transporters.
Rudnick G, Wall S. Non-neurotoxic amphetamine derivatives release serotonin through serotonin transporters. Molecular Pharmacology 1993, 43: 271-6. PMID: 8429828.Peer-Reviewed Original ResearchMeSH KeywordsAmphetaminesBlood PlateletsCarrier ProteinsCell MembraneChromaffin GranulesHumansImipramineIn Vitro TechniquesIndansMembrane GlycoproteinsMembrane Transport ProteinsMethamphetamineModels, BiologicalNerve EndingsNerve Tissue ProteinsRadioligand AssaySerotoninSerotonin Plasma Membrane Transport ProteinsConceptsChromaffin granule membrane vesiclesPlasma membrane vesiclesMembrane vesiclesPlatelet plasma membrane vesiclesPlasma membrane transportersSerotonin transportBiogenic amine transportersMembrane transportersAmine transportersTransportersVesiclesDopamine transporterSerotonin transporterModel systemHalf-maximal concentrationNerve terminalsTransmembraneCocaine analogBindingSimilar concentrationsHigh concentrationsCellsTransport
1992
p-Chloroamphetamine induces serotonin release through serotonin transporters.
Rudnick G, Wall S. p-Chloroamphetamine induces serotonin release through serotonin transporters. Biochemistry 1992, 31: 6710-8. PMID: 1322169, DOI: 10.1021/bi00144a010.Peer-Reviewed Original ResearchMeSH KeywordsBinding, CompetitiveBiological TransportBlood PlateletsCarrier ProteinsCell MembraneChloridesHumansHydrogen-Ion ConcentrationImipramineKineticsLithiumLithium ChlorideMembrane GlycoproteinsMembrane Transport ProteinsNerve Tissue ProteinsP-ChloroamphetamineRadioisotope Dilution TechniqueSerotoninSerotonin Plasma Membrane Transport ProteinsTritiumConceptsPlasma membrane vesiclesMembrane vesiclesChromaffin granule membrane vesiclesVesicular amine transporterATP hydrolysisBovine adrenal chromaffin granulesSerotonin transporterAdrenal chromaffin granulesPrevents accumulationAmine transportersPresence of Mg2TransportersVesiclesChromaffin granulesModel systemDelta pHHuman plateletsATPEffluxManner characteristicAccumulationTransmembraneNaCl gradientPCA's abilityP-chloroamphetamineThe molecular mechanism of "ecstasy" [3,4-methylenedioxy-methamphetamine (MDMA)]: serotonin transporters are targets for MDMA-induced serotonin release.
Rudnick G, Wall S. The molecular mechanism of "ecstasy" [3,4-methylenedioxy-methamphetamine (MDMA)]: serotonin transporters are targets for MDMA-induced serotonin release. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 1817-1821. PMID: 1347426, PMCID: PMC48544, DOI: 10.1073/pnas.89.5.1817.Peer-Reviewed Original ResearchConceptsPlasma membrane vesiclesMembrane vesiclesAmine transportersVesicular amine transporterBiogenic amine transportersSecretory vesiclesPlasma membraneATP hydrolysisMolecular mechanismsBovine adrenal chromaffin granulesSerotonin transporterAdrenal chromaffin granulesTransportersDirect interactionVesiclesChromaffin granulesHuman plateletsManner characteristicEffluxTransmembraneATPMDMA actionMechanismBindingMembranePlatelet serotonin transporter
Rudnick G, Humphreys C. Platelet serotonin transporter. Methods In Enzymology 1992, 215: 213-224. PMID: 1435323, DOI: 10.1016/0076-6879(92)15065-k.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsAdenosine TriphosphateAnimalsBiological TransportBlood PlateletsCarrier ProteinsCell FractionationCell MembraneCell SeparationCentrifugationHumansImipramineIndicators and ReagentsMembrane GlycoproteinsMembrane Transport ProteinsNerve Tissue ProteinsRadioligand AssaySerotoninSerotonin Plasma Membrane Transport ProteinsSwineTritium
1991
Substrate and inhibitor binding and translocation by the platelet plasma membrane serotonin transporter
Humphreys C, Beidler D, Rudnick G. Substrate and inhibitor binding and translocation by the platelet plasma membrane serotonin transporter. Biochemical Society Transactions 1991, 19: 95-98. PMID: 2037207, DOI: 10.1042/bst0190095.Peer-Reviewed Original Research
1989
2-Iodoimipramine, a novel ligand for the serotonin transporter.
Humphreys C, Cassel D, Rudnick G. 2-Iodoimipramine, a novel ligand for the serotonin transporter. Molecular Pharmacology 1989, 36: 620-6. PMID: 2811859.Peer-Reviewed Original ResearchZwitterionic and Anionic Forms of a Serotonin Analog as Transport Substrates
Rudnick G, Kirk K, Fishkes H, Schuldiner S. Zwitterionic and Anionic Forms of a Serotonin Analog as Transport Substrates. Journal Of Biological Chemistry 1989, 264: 14865-14868. PMID: 2549040, DOI: 10.1016/s0021-9258(18)63781-0.Peer-Reviewed Original ResearchConceptsBiogenic amine transportersAmine transportersPlasma membrane serotonin transporterSubstrate translocationBovine chromaffin granulesTransport substratesHuman blood plateletsSerotonin transportChromaffin granulesTransportersBlood plateletsSerotonin transporterKmIdentical ratesTranslocationSubstrateTransport
1988
Antidepressant binding to the porcine and human platelet serotonin transporters.
Humphreys C, Levin J, Rudnick G. Antidepressant binding to the porcine and human platelet serotonin transporters. Molecular Pharmacology 1988, 33: 657-63. PMID: 3380080.Peer-Reviewed Original Research
1985
Serotonin Transport by Platelet Plasma and Granule Membranes
RUDNICK G, HUMPHREYS C, DEAN G. Serotonin Transport by Platelet Plasma and Granule Membranes. Annals Of The New York Academy Of Sciences 1985, 456: 277-278. PMID: 2418730, DOI: 10.1111/j.1749-6632.1985.tb14876.x.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
1984
The hydrogen ion-pumping adenosine triphosphatase of platelet dense granule membrane. Differences from F1F0- and phosphoenzyme-type ATPases.
Dean G, Fishkes H, Nelson P, Rudnick G. The hydrogen ion-pumping adenosine triphosphatase of platelet dense granule membrane. Differences from F1F0- and phosphoenzyme-type ATPases. Journal Of Biological Chemistry 1984, 259: 9569-9574. PMID: 6204985, DOI: 10.1016/s0021-9258(17)42738-4.Peer-Reviewed Original ResearchConceptsGranule ATPaseGranule membranesMitochondrial membraneATP hydrolysisIon-translocating ATPasesEnergy-transducing membranesDense granule membraneIdentical assay conditionsF1F0-ATPasesSubstrate specificityPlasma membraneMitochondrial ATPaseInhibitor sensitivityATPasesATPaseATPase activityConcentrations of NaN3Cation requirementATPase moleculesMembraneN-ethylmaleimideAssay conditionsAdenosine triphosphataseEfrapeptinOligomycin
1983
The serotonin transporter-imipramine "receptor".
Talvenheimo J, Fishkes H, Nelson P, Rudnick G. The serotonin transporter-imipramine "receptor". Journal Of Biological Chemistry 1983, 258: 6115-6119. PMID: 6853478, DOI: 10.1016/s0021-9258(18)32380-9.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding, CompetitiveBiological TransportBlood PlateletsCarrier ProteinsCell MembraneImipramineSerotoninSodiumSwineSodium ion requirements for serotonin transport and imipramine binding.
Rudnick G, Talvenheimo J, Fishkes H, Nelson P. Sodium ion requirements for serotonin transport and imipramine binding. Psychopharmacology Bulletin 1983, 19: 545-9. PMID: 6635128.Peer-Reviewed Original Research
1982
The role of chloride ion in platelet serotonin transport.
Nelson P, Rudnick G. The role of chloride ion in platelet serotonin transport. Journal Of Biological Chemistry 1982, 257: 6151-6155. PMID: 7076668, DOI: 10.1016/s0021-9258(20)65119-5.Peer-Reviewed Original ResearchBioenergetics of serotonin transport by membrane vesicles derived from platelet dense granules.
Fishkes H, Rudnick G. Bioenergetics of serotonin transport by membrane vesicles derived from platelet dense granules. Journal Of Biological Chemistry 1982, 257: 5671-5677. PMID: 7068613, DOI: 10.1016/s0021-9258(19)83830-9.Peer-Reviewed Original ResearchConceptsPlatelet dense granulesMembrane vesiclesSerotonin transport activitySoluble enzymatic activityPresence of ATPCarbonyl cyanide p-trifluoromethoxyphenylhydrazoneAddition of ATPOsmotic lysatesTransport activityCyanide p-trifluoromethoxyphenylhydrazoneEnzymatic activityDensity gradient centrifugationVesiclesSerotonin transportVesicle interiorDense granulesMembrane potentialP-trifluoromethoxyphenylhydrazoneGradient centrifugationATPAssay mediumAdenine nucleotidesBioenergeticsNucleotidesGranulesCoupling of transmembrane proton gradients to platelet serotonin transport.
Keyes S, Rudnick G. Coupling of transmembrane proton gradients to platelet serotonin transport. Journal Of Biological Chemistry 1982, 257: 1172-1176. PMID: 7056713, DOI: 10.1016/s0021-9258(19)68170-6.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportBlood PlateletsCell MembraneHydrogen-Ion ConcentrationKineticsPotassiumSerotoninSwineConceptsPlasma membrane vesiclesSerotonin transportTransmembrane proton gradientPlatelet plasma membraneTransmembrane ion gradientsApparent competitionPlasma membraneMembrane vesiclesProton gradientIon gradientsSerotonin accumulationSole driving forceVesiclesAccumulationSerotonin transporterPH differencePhysiological pHTransportersTransportPH stimulationMembraneGradient
1981
Anion-dependent sodium ion conductance of platelet plasma membranes.
Nelson P, Rudnick G. Anion-dependent sodium ion conductance of platelet plasma membranes. Biochemistry 1981, 20: 4246-9. PMID: 6269584, DOI: 10.1021/bi00518a002.Peer-Reviewed Original ResearchInhibition of platelet serotonin transport by propranolol.
Rudnick G, Bencuya R, Nelson P, Zito R. Inhibition of platelet serotonin transport by propranolol. Molecular Pharmacology 1981, 20: 118-23. PMID: 7290078.Peer-Reviewed Original Research