2022
SILAC-IodoTMT for Assessment of the Cellular Proteome and Its Redox Status
Vajrychova M, Salovska B, Pimkova K, Fabrik I, Hodny Z. SILAC-IodoTMT for Assessment of the Cellular Proteome and Its Redox Status. Methods In Molecular Biology 2022, 2603: 259-268. PMID: 36370286, DOI: 10.1007/978-1-0716-2863-8_21.Peer-Reviewed Original ResearchMeSH KeywordsChromatography, LiquidCysteineIsotope LabelingOxidation-ReductionProteomeProteomicsTandem Mass SpectrometryConceptsHigh-resolution mass spectrometryIodoacetyl tandem mass tagLiquid chromatography separationMass spectrometry-based approachQuantification of proteinsStable isotope labelingChromatography separationTandem mass tagsMass spectrometryCysteine modificationCellular proteomeGlobal proteomeIsotope labelingMass tagsModification levelsAmino acidsRedox statusProteomeCell culturesSpectrometrySILACSeparationProteinAcidTags
2019
Quantification of cellular protein and redox imbalance using SILAC-iodoTMT methodology
Vajrychova M, Salovska B, Pimkova K, Fabrik I, Tambor V, Kondelova A, Bartek J, Hodny Z. Quantification of cellular protein and redox imbalance using SILAC-iodoTMT methodology. Redox Biology 2019, 24: 101227. PMID: 31154163, PMCID: PMC6545335, DOI: 10.1016/j.redox.2019.101227.Peer-Reviewed Original ResearchMeSH KeywordsChromatography, LiquidCysteineHydrogen PeroxideOxidation-ReductionOxidative StressPeroxiredoxinsProteomeProteomicsTandem Mass SpectrometryConceptsCellular proteomeCysteine oxidationProtein thiol residuesRedox statusCellular protein expressionRedox changesCellular redox statusOrganismal physiologyVersatile experimental approachProtein functionCellular proteinsTranscription factorsRedox homeostasisReporter ion quantificationOxidation processMetabolic labelingFunctional analysisProtein turnoverNew analytical methodThiol residuesIon quantificationRedox alterationsRedox modulatingBiological relevanceRedox imbalance