2021
Peroxiredoxin 6 protects irradiated cells from oxidative stress and shapes their senescence-associated cytokine landscape
Salovska B, Kondelova A, Pimkova K, Liblova Z, Pribyl M, Fabrik I, Bartek J, Vajrychova M, Hodny Z. Peroxiredoxin 6 protects irradiated cells from oxidative stress and shapes their senescence-associated cytokine landscape. Redox Biology 2021, 49: 102212. PMID: 34923300, PMCID: PMC8688892, DOI: 10.1016/j.redox.2021.102212.Peer-Reviewed Original ResearchConceptsSenescence-associated secretory phenotypePeroxiredoxin 6Senescent cellsIrreversible cell cycle arrestProtein secretory pathwayStress-induced cell deathProteome-level changesProteome-wide analysisCyclin-dependent kinasesProtein sulfhydryl oxidationOxidative stressPeroxiredoxin family membersExtracellular matrix proteinsComplex stress responseHTERT-RPE-1Cell cycle arrestSecretory pathwayRadiation-induced senescenceRedox homeostasisCellular senescenceDependent kinasesSecretome analysisStress responseSenescent phenotypeAntioxidant proteins
2019
Quantification of cellular protein and redox imbalance using SILAC-iodoTMT methodology
Vajrychova M, Salovska B, Pimkova K, Fabrik I, Tambor V, Kondelova A, Bartek J, Hodny Z. Quantification of cellular protein and redox imbalance using SILAC-iodoTMT methodology. Redox Biology 2019, 24: 101227. PMID: 31154163, PMCID: PMC6545335, DOI: 10.1016/j.redox.2019.101227.Peer-Reviewed Original ResearchConceptsCellular proteomeCysteine oxidationProtein thiol residuesRedox statusCellular protein expressionRedox changesCellular redox statusOrganismal physiologyVersatile experimental approachProtein functionCellular proteinsTranscription factorsRedox homeostasisReporter ion quantificationOxidation processMetabolic labelingFunctional analysisProtein turnoverNew analytical methodThiol residuesIon quantificationRedox alterationsRedox modulatingBiological relevanceRedox imbalance