2021
Peroxiredoxin 6 protects irradiated cells from oxidative stress and shapes their senescence-associated cytokine landscape
Salovska B, Kondelova A, Pimkova K, Liblova Z, Pribyl M, Fabrik I, Bartek J, Vajrychova M, Hodny Z. Peroxiredoxin 6 protects irradiated cells from oxidative stress and shapes their senescence-associated cytokine landscape. Redox Biology 2021, 49: 102212. PMID: 34923300, PMCID: PMC8688892, DOI: 10.1016/j.redox.2021.102212.Peer-Reviewed Original ResearchConceptsSenescence-associated secretory phenotypePeroxiredoxin 6Senescent cellsIrreversible cell cycle arrestProtein secretory pathwayStress-induced cell deathProteome-level changesProteome-wide analysisCyclin-dependent kinasesProtein sulfhydryl oxidationOxidative stressPeroxiredoxin family membersExtracellular matrix proteinsComplex stress responseHTERT-RPE-1Cell cycle arrestSecretory pathwayRadiation-induced senescenceRedox homeostasisCellular senescenceDependent kinasesSecretome analysisStress responseSenescent phenotypeAntioxidant proteins
2014
Radiosensitization of Human Leukemic HL-60 Cells by ATR Kinase Inhibitor (VE-821): Phosphoproteomic Analysis
Šalovská B, Fabrik I, Ďurišová K, Link M, Vávrová J, Řezáčová M, Tichý A. Radiosensitization of Human Leukemic HL-60 Cells by ATR Kinase Inhibitor (VE-821): Phosphoproteomic Analysis. International Journal Of Molecular Sciences 2014, 15: 12007-12026. PMID: 25003641, PMCID: PMC4139827, DOI: 10.3390/ijms150712007.Peer-Reviewed Original ResearchConceptsDNA-dependent protein kinaseVE-821HL-60 cellsNano-liquid chromatography-tandem mass spectrometry analysisCell cycleSequence motif analysisDNA damage responseRadiation-induced double-strand breaksATR kinase inhibitorsDNA damage repairDNA damaging agentsHuman leukemic HL-60 cellsDouble-strand breaksSpecific ATR inhibitorActivity of kinasesInhibitor VE-821Leukemic HL-60 cellsCell cycle arrestQuantitative phosphoproteomicsATR kinaseMotif analysisPhosphorylation sitesCellular processesDamage responsePhosphoproteomic analysis