2001
Regulation of Ins(1,4,5)P3 receptor isoforms by endogenous modulators
Thrower E, Hagar R, Ehrlich B. Regulation of Ins(1,4,5)P3 receptor isoforms by endogenous modulators. Trends In Pharmacological Sciences 2001, 22: 580-586. PMID: 11698102, DOI: 10.1016/s0165-6147(00)01809-5.Peer-Reviewed Original ResearchHuman disease: Calcium signaling in polycystic kidney disease
Somlo S, Ehrlich B. Human disease: Calcium signaling in polycystic kidney disease. Current Biology 2001, 11: r356-r360. PMID: 11369247, DOI: 10.1016/s0960-9822(01)00193-2.Peer-Reviewed Original Research
2000
Conservation of localization patterns of IP3 receptor type 1 in cerebellar Purkinje cells across vertebrate species
Koulen P, Janowitz T, Johnston L, Ehrlich B. Conservation of localization patterns of IP3 receptor type 1 in cerebellar Purkinje cells across vertebrate species. Journal Of Neuroscience Research 2000, 61: 493-499. PMID: 10956418, DOI: 10.1002/1097-4547(20000901)61:5<493::aid-jnr3>3.0.co;2-9.Peer-Reviewed Original ResearchConceptsHigh expression levelsVertebrate speciesDifferent vertebrate speciesPhylogenetic developmentNumber of speciesExpression levelsType 1 proteinIP3 receptor type 1Distribution of inositolLocalization patternsMolecular levelPurkinje cellsFluorescence immunocytochemistryCellular levelSpeciesFunctional differencesPolyclonal antiserumAdult cerebellumCerebellar Purkinje cellsReceptor type 1CellsConservationIntracellular calciumOrganismsPurkinje cell dendritesRegulation of the Type III InsP3 Receptor by InsP3 and ATP
Hagar R, Ehrlich B. Regulation of the Type III InsP3 Receptor by InsP3 and ATP. Biophysical Journal 2000, 79: 271-278. PMID: 10866953, PMCID: PMC1300931, DOI: 10.1016/s0006-3495(00)76289-8.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAllosteric RegulationAnimalsBinding, CompetitiveCalcium ChannelsDose-Response Relationship, DrugEndoplasmic ReticulumInositol 1,4,5-TrisphosphateInositol 1,4,5-Trisphosphate ReceptorsInsulinomaMicrosomesProtein IsoformsRatsReceptors, Cytoplasmic and NuclearSubstrate SpecificityTumor Cells, CulturedConceptsType IIIChannel activityIntracellular calciumMaximal channel activityATP concentrationProlonged stimulationDependent inhibitionAllosteric modulationInsP3 receptorEnhanced channel activityType IOpen probabilityOpen timeReceptorsHigh ATP concentrationsHigh levelsATPLow ATP concentrationsIsoformsActivity
1999
Calcium signaling molecules in human cerebellum at midgestation and in ataxia
Zecevic N, Milosevic A, Ehrlich B. Calcium signaling molecules in human cerebellum at midgestation and in ataxia. Early Human Development 1999, 54: 103-116. PMID: 10213289, DOI: 10.1016/s0378-3782(98)00090-5.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedBlotting, WesternCadaverCalcium ChannelsCalcium-Binding ProteinsCell DifferentiationCerebellar AtaxiaCerebellumEmbryonic and Fetal DevelopmentHumansImmunohistochemistryInositol 1,4,5-Trisphosphate ReceptorsMicroscopy, FluorescencePurkinje CellsReceptors, Cytoplasmic and NuclearRyanodine Receptor Calcium Release ChannelSynapsesConceptsPurkinje cell layerImmune reactionsIntracellular Ca2Precise topographic connectionsInositol trisphosphate receptor type 1Positive immune reactionHuman cerebellumCell layerReceptor type 1Expression of Ca2Channels/receptorsNeuronal typesAdult brainTrisphosphate receptor type 1Cerebellar cortexDiseased brainTopographic connectionsIntrauterine stagesYounger ageType 1Brain developmentInternal storesImmunocytochemical methodsCerebellumAtaxia
1998
Type III InsP3 receptor channel stays open in the presence of increased calcium
Hagar R, Burgstahler A, Nathanson M, Ehrlich B. Type III InsP3 receptor channel stays open in the presence of increased calcium. Nature 1998, 396: 81-84. PMID: 9817204, PMCID: PMC2825878, DOI: 10.1038/23954.Peer-Reviewed Original ResearchCharacterization of the ryanodine receptor/channel of invertebrate muscle
Quinn K, Castellani L, Ondrias K, Ehrlich B. Characterization of the ryanodine receptor/channel of invertebrate muscle. American Journal Of Physiology 1998, 274: r494-r502. PMID: 9486309, DOI: 10.1152/ajpregu.1998.274.2.r494.Peer-Reviewed Original ResearchConceptsInvertebrate musclesRyanodine receptor/channelVertebrate skeletal muscleDiverse organismsCalcium release channel/ryanodine receptorRelease channel/ryanodine receptorChannel/ryanodine receptorSingle-channel conductanceReceptor/channelElectron microscopic analysisRyanodine receptorSkeletal muscleRelease channelCytoplasmic Ca2Single-channel currentsSpecific ryanodine bindingChannel conductanceSarcoplasmic reticulumRyRsRuthenium redInvertebratesCa2MammalianRyanodine bindingOrganisms
1997
Inositol 1,4,5-Trisphosphate (InsP3) and Calcium Interact to Increase the Dynamic Range of InsP3 Receptor-dependent Calcium Signaling
Kaftan E, Ehrlich B, Watras J. Inositol 1,4,5-Trisphosphate (InsP3) and Calcium Interact to Increase the Dynamic Range of InsP3 Receptor-dependent Calcium Signaling. The Journal Of General Physiology 1997, 110: 529-538. PMID: 9348325, PMCID: PMC2229389, DOI: 10.1085/jgp.110.5.529.Peer-Reviewed Original ResearchRegulation of Intracellular Calcium Release Channel Function by Arachidonic Acid and Leukotriene B4
Striggow F, Ehrlich B. Regulation of Intracellular Calcium Release Channel Function by Arachidonic Acid and Leukotriene B4. Biochemical And Biophysical Research Communications 1997, 237: 413-418. PMID: 9268725, DOI: 10.1006/bbrc.1997.7152.Peer-Reviewed Original ResearchConceptsLeukotriene B4Arachidonic acidCalcium release channelNM leukotriene B4Intracellular calcium concentrationRelease channelIntracellular calcium release channelsTrisphosphate-gated channelsRelease channel functionCalcium concentrationRyanodine receptorChannel functionCell typesSpecific mobilizationB4RyRsAgonistsConcerted actionMethanethiosulfonate Derivatives Inhibit Current through the Ryanodine Receptor/Channel
Quinn K, Ehrlich B. Methanethiosulfonate Derivatives Inhibit Current through the Ryanodine Receptor/Channel. The Journal Of General Physiology 1997, 109: 255-264. PMID: 9041453, PMCID: PMC2220055, DOI: 10.1085/jgp.109.2.255.Peer-Reviewed Original Research
1996
Ligand-gated calcium channels inside and out
Striggow F, Ehrlich B. Ligand-gated calcium channels inside and out. Current Opinion In Cell Biology 1996, 8: 490-495. PMID: 8791458, DOI: 10.1016/s0955-0674(96)80025-1.Peer-Reviewed Original ResearchConceptsLigand-gated calcium channelsStore-operated channelsCalcium-permeable channelsIntracellular membranesFunctional characterizationPlasma membraneAllosteric mechanismAssociated proteinsTrisphosphate receptorCalcium releaseRyanodine receptorIntracellular storesIntracellular calciumCalcium channelsMembraneReceptorsProteinTypes of channelsInositolReleaseEffects of Rapamycin on Ryanodine Receptor/Ca2+-Release Channels From Cardiac Muscle
Kaftan E, Marks A, Ehrlich B. Effects of Rapamycin on Ryanodine Receptor/Ca2+-Release Channels From Cardiac Muscle. Circulation Research 1996, 78: 990-997. PMID: 8635249, DOI: 10.1161/01.res.78.6.990.Peer-Reviewed Original ResearchConceptsRelease channelFK506-binding proteinCardiac muscleLong-term depressionEffect of rapamycinChannel functionProlyl isomerase activityRelease of Ca2Ryanodine receptor/Ca2Immunosuppressant drugsSkeletal muscle isoformCardiac RyRIsomerase activityRyanodine receptorRapamycinRyRsChannel familyImportant regulatory roleCis-trans peptidylOpen probabilitySubmicromolar concentrationsCell typesDrugsMuscleMuscle isoform
1995
Functional properties of intracellular calcium-release channels
Ehrlich B. Functional properties of intracellular calcium-release channels. Current Opinion In Neurobiology 1995, 5: 304-309. PMID: 7580152, DOI: 10.1016/0959-4388(95)80042-5.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCalcium ChannelsModels, BiologicalMuscle ProteinsRyanodine Receptor Calcium Release ChannelConceptsIntracellular calcium release channelsCalcium release channelLarge ion channelsCellular rolesCytoplasmic proteinsMolecular mechanismsRegulatory sitesTrisphosphate receptorFunctional interactionIon channelsRegulatory processesMajor classesRyanodine receptorProteinRecent studiesFunctional propertiesReceptorsCytoplasmInositol
1994
The pharmacology of intracellular Ca2+-release channels
Ehrlich B, Kaftan E, Bezprozvannaya S, Bezprozvanny I. The pharmacology of intracellular Ca2+-release channels. Trends In Pharmacological Sciences 1994, 15: 145-149. PMID: 7754532, DOI: 10.1016/0165-6147(94)90074-4.Peer-Reviewed Original ResearchStabilization of calcium release channel (ryanodine receptor) function by FK506-binding protein
Brillantes A, Ondrias K, Scott A, Kobrinsky E, Ondriašová E, Moschella M, Jayaraman T, Landers M, Ehrlich B, Marks A. Stabilization of calcium release channel (ryanodine receptor) function by FK506-binding protein. Cell 1994, 77: 513-523. PMID: 7514503, DOI: 10.1016/0092-8674(94)90214-3.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaffeineCalciumCalcium ChannelsCarrier ProteinsCell LineCloning, MolecularGene ExpressionHeat-Shock ProteinsIon Channel GatingIsometric ContractionLipid BilayersMuscle ProteinsPolyenesRabbitsRatsRecombinant ProteinsRNA, MessengerRuthenium RedRyanodineRyanodine Receptor Calcium Release ChannelSarcoplasmic ReticulumSirolimusTacrolimusTacrolimus Binding ProteinsConceptsCellular functionsFK506-binding proteinNatural cellular functionsRelease channel functionInsect cellsProline isomeraseIsomerase activityEndoplasmic reticulumChannel functionFKBP12Channel complexRyanodine receptorCytosolic receptorRelease channel complexRelease channelRapamycinProteinFunctional Ca2Open probabilityCaffeine activationIntracellular Ca2Mean open timeImmunosuppressant drugsCopurifiesReceptors
1993
ATP modulates the function of inositol 1,4,5-trisphosphate-gated channels at two sites
Bezprozvanny I, Ehrlich B. ATP modulates the function of inositol 1,4,5-trisphosphate-gated channels at two sites. Neuron 1993, 10: 1175-1184. PMID: 7686381, DOI: 10.1016/0896-6273(93)90065-y.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCalciumCalcium ChannelsCerebellumDogsEndoplasmic ReticulumInositol 1,4,5-TrisphosphateInositol 1,4,5-Trisphosphate ReceptorsIon Channel GatingIon ChannelsKineticsLipid BilayersMathematicsMicrosomesModels, NeurologicalReceptors, Cell SurfaceReceptors, Cytoplasmic and NuclearSignal TransductionConceptsPresence of IP3Effect of ATPTrisphosphate-gated channelsIntracellular Ca2Average durationAllosteric modulationSingle-channel levelFunction of inositolIP3 receptorChannel activityCell viabilityReceptorsTrisphosphate receptorIP3Channel openingPermeable channelsCa2ATPInositolAddition of ATPNonhydrolyzable ATP analogChannel conductanceChannel incorporation
1991
Comparison of calcium release from sarcoplasmic reticulum of slow and fast twitch muscles
Lee Y, Ondrias K, Duhl A, Ehrlich B, Kim D. Comparison of calcium release from sarcoplasmic reticulum of slow and fast twitch muscles. The Journal Of Membrane Biology 1991, 122: 155-163. PMID: 1716686, DOI: 10.1007/bf01872638.Peer-Reviewed Original ResearchConceptsFast sarcoplasmic reticulumFast-twitch muscleSarcoplasmic reticulumRelease channelSlow sarcoplasmic reticulumTwitch muscleFast musclesSR vesiclesTypes of muscleCalcium releaseMechanism of Ca2MuscleScatchard analysisRyanodine receptorFunctional assaysRuthenium redSingle Ca2Single-channel conductanceOpen probabilityMolecular weight proteinsCa2Closed timeHigh molecular weight proteinsFunctional differencesBell-shaped calcium-response curves of lns(l,4,5)P3- and calcium-gated channels from endoplasmic reticulum of cerebellum
Bezprozvanny L, Watras J, Ehrlich B. Bell-shaped calcium-response curves of lns(l,4,5)P3- and calcium-gated channels from endoplasmic reticulum of cerebellum. Nature 1991, 351: 751-754. PMID: 1648178, DOI: 10.1038/351751a0.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCalciumCalcium ChannelsCell MembraneCerebellumCytoplasmDogsEndoplasmic ReticulumFeedbackInositol 1,4,5-TrisphosphateInositol 1,4,5-Trisphosphate ReceptorsIon Channel GatingLipid BilayersReceptors, Cell SurfaceReceptors, CholinergicReceptors, Cytoplasmic and NuclearRyanodine Receptor Calcium Release ChannelConceptsCalcium-gated channelsRyanodine receptor/channelEndoplasmic reticulum vesiclesLigand sensitivityEndoplasmic reticulumReceptor/channelCalcium-activated channelChannel typesSame cellsIntracellular calcium regulationCalcium regulationPlanar bilayersCytoplasmic calciumIntracellular storesOpen probabilityCanine cerebellumComplex patternsDifferent responsesAdenine nucleotidesRelease of calciumFunctional propertiesRuthenium redMaximum activityInsP3Cells