2010
The Yb Body, a Major Site for Piwi-associated RNA Biogenesis and a Gateway for Piwi Expression and Transport to the Nucleus in Somatic Cells*
Qi H, Watanabe T, Ku HY, Liu N, Zhong M, Lin H. The Yb Body, a Major Site for Piwi-associated RNA Biogenesis and a Gateway for Piwi Expression and Transport to the Nucleus in Somatic Cells*. Journal Of Biological Chemistry 2010, 286: 3789-3797. PMID: 21106531, PMCID: PMC3030380, DOI: 10.1074/jbc.m110.193888.Peer-Reviewed Original ResearchConceptsYb bodiesSomatic cellsPiRNA pathwayGerm lineEndo-siRNA pathwaySomatic niche cellsTudor-like domainGonadal somatic cellsPutative RNA helicaseCo-immunoprecipitation experimentsSomatic stem cellsFlamenco locusSomatic piRNAsPiRNA biogenesisEndo-siRNAsRNA biogenesisRNA pathwaysPiwi expressionRNA helicaseNovel proteinPiwiNiche cellsMolecular basisStem cellsBiogenesis
2006
The Role of PIWI and the miRNA Machinery in Drosophila Germline Determination
Megosh HB, Cox DN, Campbell C, Lin H. The Role of PIWI and the miRNA Machinery in Drosophila Germline Determination. Current Biology 2006, 16: 1884-1894. PMID: 16949822, DOI: 10.1016/j.cub.2006.08.051.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArgonaute ProteinsBody PatterningCell DifferentiationDEAD-box RNA HelicasesDrosophilaDrosophila ProteinsEmbryo, NonmammalianEmbryonic DevelopmentFragile X Mental Retardation ProteinGene Expression Regulation, DevelopmentalGerm CellsMicroRNAsProteinsRibonuclease IIIRNA HelicasesRNA, Small InterferingRNA-Induced Silencing ComplexConceptsGermline determinationDicer-1Dicer-2MiRNA pathwayRole of PiwiReduced PGC numbersNumber of PGCsMiRISC complexesRasiRNA pathwayGermline specificationDrosophila PiwiSiRNA pathwayAbdominal patterningVasa expressionTranslational regulationPGC numberPiwiMiRNA machineryGerm plasmMolecular activityPolar granuleDFMRPCell formationOSKMaternal component
2004
Mili, a mammalian member of piwi family gene, is essential for spermatogenesis
Kuramochi-Miyagawa S, Kimura T, Ijiri TW, Isobe T, Asada N, Fujita Y, Ikawa M, Iwai N, Okabe M, Deng W, Lin H, Matsuda Y, Nakano T. Mili, a mammalian member of piwi family gene, is essential for spermatogenesis. Development 2004, 131: 839-849. PMID: 14736746, DOI: 10.1242/dev.00973.Peer-Reviewed Original ResearchConceptsPiwi family genesFamily genesPrimordial germ cell developmentPost-transcriptional regulationGerm cell developmentGerm cell productionPhysical binding propertiesMammalian homologPIWI domainMammalian membersTranslational regulationMouse homologDifferentiation of spermatocytesEarly prophaseMILIFunctional associationTargeted mutationsCell developmentFirst meiosisGenesPiwiEarly pachyteneStem cellsSpermatocyte stageHomolog