2020
Characterization of circulating and cultured Tfh-like cells in sickle cell disease in relation to red blood cell alloimmunization status
Balbuena-Merle R, Santhanakrishnan M, Devine L, Gibb DR, Tormey CA, Siddon AJ, Curtis SA, Gallagher PG, Weinstein JS, Hendrickson JE. Characterization of circulating and cultured Tfh-like cells in sickle cell disease in relation to red blood cell alloimmunization status. Transfusion And Apheresis Science 2020, 59: 102778. PMID: 32439490, PMCID: PMC7483805, DOI: 10.1016/j.transci.2020.102778.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnemia, Sickle CellErythrocyte TransfusionFemaleHumansMaleT-Lymphocyte SubsetsTransfusion MedicineConceptsTfh-like cellsNaïve CD4 T cellsSickle cell diseaseCD4 T cellsCD4 T cell subsetsT cell subsetsT cellsCell diseaseRed blood cell alloimmunizationPeripheral blood mononuclear cellsBlood mononuclear cellsCD3/CD28Electronic medical recordsAlloimmunization statusHLA alloantibodiesRBC autoantibodiesRBC alloantibodiesFollicular helperIL-12Mononuclear cellsMedical recordsIL-7Antigen specificityB cellsAlloantibodies
2015
Transporting down the road to dehydration
Gallagher PG. Transporting down the road to dehydration. Blood 2015, 126: 2775-2776. PMID: 26705342, DOI: 10.1182/blood-2015-10-675488.Peer-Reviewed Original ResearchPomalidomide reverses γ-globin silencing through the transcriptional reprogramming of adult hematopoietic progenitors
Dulmovits BM, Appiah-Kubi AO, Papoin J, Hale J, He M, Al-Abed Y, Didier S, Gould M, Husain-Krautter S, Singh SA, Chan KW, Vlachos A, Allen SL, Taylor N, Marambaud P, An X, Gallagher PG, Mohandas N, Lipton JM, Liu JM, Blanc L. Pomalidomide reverses γ-globin silencing through the transcriptional reprogramming of adult hematopoietic progenitors. Blood 2015, 127: 1481-1492. PMID: 26679864, PMCID: PMC4797024, DOI: 10.1182/blood-2015-09-667923.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnemia, Sickle CellBeta-GlobinsCarrier ProteinsErythroid Precursor CellsErythropoiesisFetal HemoglobinGamma-GlobinsGene Expression Regulation, DevelopmentalGenetic VectorsHematopoietic Stem CellsHistone DemethylasesHumansIkaros Transcription FactorKruppel-Like Transcription FactorsLentivirusMultiple MyelomaNeoplasm ProteinsNuclear ProteinsProteasome Endopeptidase ComplexRepressor ProteinsRNA InterferenceRNA, Small InterferingSOXD Transcription FactorsThalidomideTranscription, GeneticConceptsSickle cell anemiaCell anemiaΓ-globinThird-generation immunomodulatory drugAdult human erythroblastsMultiple myeloma patientsHematopoietic progenitorsΓ-globin levelsΓ-globin repressionCurrent therapeutic strategiesErythroid differentiation programFetal hemoglobinAdult hematopoietic progenitorsPomalidomide treatmentImmunomodulatory drugsMyeloma patientsTranscriptional reprogrammingFetal hemoglobin productionTranscription networksTherapeutic strategiesDifferentiation programPomalidomideHuman erythroblastsΒ-hemoglobinopathiesGenetic ablation
2013
Fetal hemoglobin in sickle cell anemia: Genetic studies of the Arab-Indian haplotype
Ngo D, Bae H, Steinberg MH, Sebastiani P, Solovieff N, Baldwin CT, Melista E, Safaya S, Farrer LA, Al-Suliman AM, Albuali WH, Bagshi M, Naserullah Z, Akinsheye I, Gallagher P, Luo HY, Chui DH, Farrell JJ, Al-Ali AK, Alsultan A. Fetal hemoglobin in sickle cell anemia: Genetic studies of the Arab-Indian haplotype. Blood Cells Molecules And Diseases 2013, 51: 22-26. PMID: 23465615, PMCID: PMC3647015, DOI: 10.1016/j.bcmd.2012.12.005.Peer-Reviewed Original ResearchAdolescentAdultAllelesAnemia, Sickle CellArabsBeta-GlobinsCarrier ProteinsChildChild, PreschoolFetal HemoglobinGenes, mybGTP-Binding ProteinsHaplotypesHemoglobin, SickleHomeodomain ProteinsHSP70 Heat-Shock ProteinsHumansKruppel-Like Transcription FactorsLocus Control RegionMiddle AgedMutationNuclear ProteinsPeptide Elongation FactorsPolymorphism, GeneticPromoter Regions, GeneticRepressor ProteinsSequence Analysis, DNATranscription FactorsYoung Adult
2010
Determinants of erythrocyte hydration
Rinehart J, Gulcicek EE, Joiner CH, Lifton RP, Gallagher PG. Determinants of erythrocyte hydration. Current Opinion In Hematology 2010, 17: 191-197. PMID: 20182354, PMCID: PMC4155397, DOI: 10.1097/moh.0b013e32833800d0.Peer-Reviewed Original ResearchMeSH KeywordsAnemia, Sickle CellAnimalsBody WaterErythrocytesHomeostasisHumansSignal TransductionWater-Electrolyte BalanceConceptsSickle cell diseaseErythrocyte hydrationCell diseaseNew therapeutic targetsGardos channel activationClinical complicationsPrimary disorderSecondary disordersTherapeutic targetErythrocyte dehydrationDisease preventionRelated disordersSolute homeostasisBiologic studiesDisordersChannel activationKCl cotransportDiseaseErythrocyte waterHomeostasisErythrocytesAnion transportersPremature destructionRecent studiesComplications
2005
Multiple isoforms of the KC1 cotransporter are expressed in sickle and normal erythroid cells
Crable SC, Hammond SM, Papes R, Rettig RK, Zhou GP, Gallagher PG, Joiner CH, Anderson KP. Multiple isoforms of the KC1 cotransporter are expressed in sickle and normal erythroid cells. Experimental Hematology 2005, 33: 624-631. PMID: 15911086, DOI: 10.1016/j.exphem.2005.02.006.Peer-Reviewed Original ResearchConceptsErythroid cellsKCC isoformsMultiple isoformsSplicing variantsStructure/function studiesKCl cotransporterWild-type cellsHuman erythroid cellsDifferentiated erythroid precursorsCl-dependent K fluxTransient transfection experimentsNormal erythroid cellsIsoform expression patternCotransporter activityRed cellsKCC genesExpression patternsReticulocyte maturationHuman red cellsKCC1 geneTransfection experimentsReverse transcriptase-PCRSplice variantsRelative abundanceGenes
2001
Modification of Erythrocyte Hydration in the Treatment of Sickle Cell Disease
Zhou G, Anderson K, Joiner C, Gallagher P. Modification of Erythrocyte Hydration in the Treatment of Sickle Cell Disease. Blood Cells Molecules And Diseases 2001, 27: 65-68. PMID: 11358362, DOI: 10.1006/bcmd.2000.0362.Peer-Reviewed Original Research