2005
The Human Alpha Hemoglobin Stabilizing Protein (AHSP) Gene Locus in EKLF-Deficient Erythroid Cells.
Pilon A, Zhou D, Weiss M, Townes T, Bodine D, Gallagher P. The Human Alpha Hemoglobin Stabilizing Protein (AHSP) Gene Locus in EKLF-Deficient Erythroid Cells. Blood 2005, 106: 1740. DOI: 10.1182/blood.v106.11.1740.1740.Peer-Reviewed Original ResearchCACCC siteErythroid cellsWild typeModifier genesDNase I hypersensitive site mappingLocal chromatin structureΒ-globin transcriptionErythroid-specific proteinΒ-globin gene promoterDNA-protein complexesFetal liver cellsTranscription factor EKLFChromatin immunoprecipitation analysisΒ-globin sequencesHypersensitive site mappingFetal liverMobility shift assaysPromoter-luciferase reporter plasmidProximal CACCC boxFetal liver DNACore promoter regionAHSP promoterDeficient fetal liver cellsEKLF geneChromatin modulators
2004
Chromatin Remodeling of the Mouse AHSP Gene Requires EKLF.
Pilon A, Wong C, Garrett-Beal L, Weiss M, Gallagher P, Bodine D. Chromatin Remodeling of the Mouse AHSP Gene Requires EKLF. Blood 2004, 104: 375. DOI: 10.1182/blood.v104.11.375.375.Peer-Reviewed Original ResearchAlpha-Hemoglobin Stabilizing ProteinDNase I hypersensitive sitesAHSP geneAHSP gene expressionHypersensitive sitesWild typeΓ-globin mRNAFetal liver cellsHistone H3Human γ-globin genesGene expressionStrong DNase I hypersensitive siteIntron 1Wild-type chromatinChromatin Remodeling ComplexErythroid-specific proteinΓ-globin genePutative promoter sequencesTranscription factor EKLFExon 1Non-coding exonsΑ-globin mRNAPosition-independent expressionMRNA initiation siteRNase protection analysis