2023
Diagnosis of acinic cell carcinoma of the salivary gland on cytology specimens: Role of NOR‐1 (NR4A3) immunohistochemistry
Meiklejohn K, Hrones M, Wang M, Prasad M, Cai G, Adeniran A, Gilani S. Diagnosis of acinic cell carcinoma of the salivary gland on cytology specimens: Role of NOR‐1 (NR4A3) immunohistochemistry. Cytopathology 2023, 34: 219-224. PMID: 36825365, DOI: 10.1111/cyt.13222.Peer-Reviewed Original ResearchGrading system for medullary thyroid carcinoma; an institutional experience
Podany P, Meiklejohn K, Garritano J, Holt E, Barbieri A, Prasad M, Gilani S. Grading system for medullary thyroid carcinoma; an institutional experience. Annals Of Diagnostic Pathology 2023, 64: 152112. PMID: 36736129, DOI: 10.1016/j.anndiagpath.2023.152112.Peer-Reviewed Original ResearchConceptsMedullary thyroid carcinomaTwo-tier grading systemDistant metastasisNuclear pleomorphismGrading systemNodal diseaseOverall survivalVascular invasionMTC casesThyroid carcinomaMedullary thyroid carcinoma casesMultivariate Cox regressionThyroid carcinoma casesCohort of casesRisk stratificationCox regressionTumor gradePathology reportsCarcinoma casesInstitutional experienceThyroid malignancyTumor fibrosisHG groupProminent nucleoliRare type
2020
Histologic Classification and Molecular Signature of Polymorphous Adenocarcinoma (PAC) and Cribriform Adenocarcinoma of Salivary Gland (CASG)
Xu B, Barbieri AL, Bishop JA, Chiosea SI, Dogan S, Di Palma S, Faquin WC, Ghossein R, Hyrcza M, Jo VY, Lewis JS, Lozada JR, Michal M, Pareja FG, Perez-Ordonez B, Prasad ML, Purgina B, Reis-Filho JS, Scognamiglio T, Sebastiao APM, Seethala RR, Skálová A, Smith SM, Tekkeşin MS, Thompson LDR, Wasseman JK, Wenig BM, Weinreb I, Katabi N. Histologic Classification and Molecular Signature of Polymorphous Adenocarcinoma (PAC) and Cribriform Adenocarcinoma of Salivary Gland (CASG). The American Journal Of Surgical Pathology 2020, 44: 545-552. PMID: 31917707, PMCID: PMC7437128, DOI: 10.1097/pas.0000000000001431.Peer-Reviewed Original ResearchMeSH KeywordsAdenocarcinomaBiomarkers, TumorBiopsyCanadaDNA Mutational AnalysisEuropeGene FusionGenetic Predisposition to DiseaseHumansIn Situ Hybridization, FluorescenceMutationObserver VariationPredictive Value of TestsReal-Time Polymerase Chain ReactionReproducibility of ResultsSalivary Gland NeoplasmsUnited StatesConceptsPapillary architectureIndeterminate featuresPolymorphous adenocarcinomaConsensus diagnosisCribriform adenocarcinomaInterobserver agreementSalivary glandsFair interobserver agreementNeck pathologistHistologic diversityHistologic classificationMorphologic spectrumDiagnostic concordanceSolid patternMolecular alterationsAdenocarcinomaGlomeruloid structuresTumorsLevel of agreementHotspot mutationsClear nucleiModerate agreementExpert pathologistsSuch molecular eventsMolecular signatures
2018
Comprehensive Genetic Analysis of Follicular Thyroid Carcinoma Predicts Prognosis Independent of Histology
Nicolson NG, Murtha TD, Dong W, Paulsson JO, Choi J, Barbieri AL, Brown TC, Kunstman JW, Larsson C, Prasad ML, Korah R, Lifton RP, Juhlin CC, Carling T. Comprehensive Genetic Analysis of Follicular Thyroid Carcinoma Predicts Prognosis Independent of Histology. The Journal Of Clinical Endocrinology & Metabolism 2018, 103: 2640-2650. PMID: 29726952, DOI: 10.1210/jc.2018-00277.Peer-Reviewed Original ResearchConceptsFollicular thyroid carcinomaThyroid carcinomaMutation burdenWorse disease-specific survivalDisease-specific survivalDifferent histopathological subtypesWorld Health Organization guidelinesSomatic mutationsNonsynonymous somatic mutationsHealth Organization guidelinesTotal mutation burdenIndependent predictorsWorse prognosisHistopathological subtypesPatient outcomesHistopathological classificationInvasive subtypesClinicopathologic parametersMutational burdenSurvival analysisSomatic copy number alterationsCopy number alterationsSubtypesOrganization guidelinesMore subclones
2017
Metastatic thyroid carcinoma without identifiable primary tumor within the thyroid gland: a retrospective study of a rare phenomenon
Xu B, Scognamiglio T, Cohen PR, Prasad ML, Hasanovic A, Tuttle RM, Katabi N, Ghossein RA. Metastatic thyroid carcinoma without identifiable primary tumor within the thyroid gland: a retrospective study of a rare phenomenon. Human Pathology 2017, 65: 133-139. PMID: 28552827, PMCID: PMC5571865, DOI: 10.1016/j.humpath.2017.05.013.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedBiomarkers, TumorCarcinomaCarcinoma, PapillaryCell DifferentiationDNA Mutational AnalysisFemaleGenetic Predisposition to DiseaseHumansImmunohistochemistryLymphatic MetastasisMaleMiddle AgedMutationNeoplasm GradingNeoplasms, Unknown PrimaryPhenotypeProto-Oncogene Proteins B-rafRetrospective StudiesThyroid Cancer, PapillaryThyroid Carcinoma, AnaplasticThyroid NeoplasmsConceptsPapillary thyroid carcinomaIdentifiable primary tumorThyroid carcinomaAnaplastic thyroid carcinomaMetastatic diseasePrimary tumorThyroid glandMetastatic papillary thyroid carcinomaEvidence of recurrenceMetastatic nodal diseaseMetastatic thyroid carcinomaTall cell variantBRAF V600E immunohistochemistryThyroid primaryNodal diseaseNeck compartmentDistant metastasisPathologic featuresRetrospective studyAnaplastic carcinomaUnknown causeMost tumorsCarcinomaPatientsThyroid tumorsPathologic Characteristics, Natural History, and Prognostic Implications of BRAFV600E Mutation in Pediatric Papillary Thyroid Carcinoma
Hardee S, Prasad ML, Hui P, Dinauer CA, Morotti RA. Pathologic Characteristics, Natural History, and Prognostic Implications of BRAFV600E Mutation in Pediatric Papillary Thyroid Carcinoma. Pediatric And Developmental Pathology 2017, 20: 206-212. PMID: 28521635, DOI: 10.1177/1093526616689628.Peer-Reviewed Original ResearchConceptsPapillary thyroid cancerPediatric papillary thyroid cancerPrognostic implicationsPediatric papillary thyroid carcinomaNegative casesBRAF-negative casesBRAF-negative patientsBRAF-positive casesTertiary medical centerAggressive clinical coursePapillary thyroid carcinomaSurgical pathology diagnosisCommon genetic aberrationsNegative patientsAggressive courseClinical coursePathologic characteristicsCase seriesClinical outcomesRetrospective reviewAggressive featuresPediatric casesRecurrence rateRetrospective studySingle institution
2016
Trans-oral Vestibular Endocrine Surgery
Udelsman R, Anuwong A, Oprea AD, Rhodes A, Prasad M, Sansone M, Brooks C, Donovan PI, Jannitto C, Carling T. Trans-oral Vestibular Endocrine Surgery. Annals Of Surgery 2016, 264: e13-e16. PMID: 27649533, DOI: 10.1097/sla.0000000000002001.Peer-Reviewed Original ResearchRecurrent Mutations of Chromatin-Remodeling Genes and Kinase Receptors in Pheochromocytomas and Paragangliomas
Toledo RA, Qin Y, Cheng ZM, Gao Q, Iwata S, Silva GM, Prasad M, Ocal IT, Rao S, Aronin N, Barontini M, Bruder J, Reddick RL, Chen Y, Aguiar RC, Dahia PL. Recurrent Mutations of Chromatin-Remodeling Genes and Kinase Receptors in Pheochromocytomas and Paragangliomas. Clinical Cancer Research 2016, 22: 2301-2310. PMID: 26700204, PMCID: PMC4854762, DOI: 10.1158/1078-0432.ccr-15-1841.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdrenal Gland NeoplasmsAdultAgedCarcinoma, NeuroendocrineChildChromatinChromatin Assembly and DisassemblyC-Mer Tyrosine KinaseExomeFemaleGerm-Line MutationGiant Cell Tumor of BoneHistone DemethylasesHistone MethyltransferasesHistone-Lysine N-MethyltransferaseHistonesHumansMaleMiddle AgedParagangliomaPheochromocytomaThyroid NeoplasmsYoung AdultConceptsHistone methylation analysisAnalysis of mutantsChromatin-remodeling genesChromatin remodelingHistone demethylasesTranscriptome sequencingFrequent genetic eventKinase geneMolecular basisPPGL susceptibility genesGenesGenetic eventsNeural crest originMethylation analysisSusceptibility genesNew cancer syndromeMost PPGLsMutationsSomatic mutationsProtein expressionCell linesDomain mutationsFGFR1 mutationsWestern blottingDriver mutationsNOTCH1 and SOX10 are Essential for Proliferation and Radiation Resistance of Cancer Stem–Like Cells in Adenoid Cystic Carcinoma
Panaccione A, Chang MT, Carbone BE, Guo Y, Moskaluk CA, Virk RK, Chiriboga L, Prasad ML, Judson B, Mehra S, Yarbrough WG, Ivanov SV. NOTCH1 and SOX10 are Essential for Proliferation and Radiation Resistance of Cancer Stem–Like Cells in Adenoid Cystic Carcinoma. Clinical Cancer Research 2016, 22: 2083-2095. PMID: 27084744, PMCID: PMC4834904, DOI: 10.1158/1078-0432.ccr-15-2208.Peer-Reviewed Original ResearchMeSH KeywordsAC133 AntigenAmyloid Precursor Protein SecretasesAnimalsBiomarkers, TumorCarcinoma, Adenoid CysticCell Line, TumorCell Transformation, NeoplasticCyclin-Dependent Kinase Inhibitor p27Disease Models, AnimalFatty Acid-Binding Protein 7Gene Expression ProfilingHeterograftsHumansLigandsMiceMice, NudeNeoplasm GradingNeoplastic Stem CellsProtein BindingRadiationRadiation ToleranceReceptor, Notch1SOXE Transcription FactorsS-Phase Kinase-Associated ProteinsTumor Cells, CulturedTumor Suppressor ProteinsConceptsCancer stem cellsRibosome biogenesisCancer stem-like cellsStem-like cellsLack of assaysCSC maintenanceMolecular markersNeural differentiationCell deathUncharacterized populationACC therapyΓ-secretase inhibitorCSC viabilityCD133-positive cancer stem cellsStem cellsCell sortingSOX10Essential roleSkp2Notch1P27Kip1Stem propertiesCell culturesImmunomagnetic cell sortingCellsNTRK fusion oncogenes in pediatric papillary thyroid carcinoma in northeast United States
Prasad ML, Vyas M, Horne MJ, Virk RK, Morotti R, Liu Z, Tallini G, Nikiforova MN, Christison-Lagay ER, Udelsman R, Dinauer CA, Nikiforov YE. NTRK fusion oncogenes in pediatric papillary thyroid carcinoma in northeast United States. Cancer 2016, 122: 1097-1107. PMID: 26784937, DOI: 10.1002/cncr.29887.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentCarcinomaCarcinoma, PapillaryChildDNA Mutational AnalysisFemaleHigh-Throughput Nucleotide SequencingHumansMaleMutationNew EnglandNuclear Pore Complex ProteinsOncogene Proteins, FusionProto-Oncogene MasProto-Oncogene ProteinsProto-Oncogene Proteins B-rafProto-Oncogene Proteins c-etsProto-Oncogene Proteins c-retReceptor, trkAReceptor, trkCRepressor ProteinsReverse Transcriptase Polymerase Chain ReactionThyroid Cancer, PapillaryThyroid Neoplasms
2015
C-Cell Neoplasia in Asymptomatic Carriers of RET Mutation in Extracellular Cysteine-Rich and Intracellular Tyrosine Kinase Domain
Abi-Raad R, Virk RK, Dinauer CA, Prasad A, Morotti RA, Breuer CK, Sosa JA, Udelsman R, Rivkees SA, Prasad ML. C-Cell Neoplasia in Asymptomatic Carriers of RET Mutation in Extracellular Cysteine-Rich and Intracellular Tyrosine Kinase Domain. Human Pathology 2015, 46: 1121-1128. PMID: 26033033, DOI: 10.1016/j.humpath.2015.04.011.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultCarcinoma, NeuroendocrineChildChild, PreschoolFemaleGenetic Predisposition to DiseaseGerm-Line MutationHeterozygoteHumansInfantMaleMiddle AgedMultiple Endocrine NeoplasiaProtein Structure, TertiaryProto-Oncogene MasProto-Oncogene Proteins c-retThyroid NeoplasmsYoung AdultConceptsMedullary thyroid carcinomaMultiple endocrine neoplasia type 2C-cell hyperplasiaAsymptomatic carriersC-cell neoplasiaRET mutationsMTC familiesHigh-risk RET mutationsIntracellular tyrosine kinase domainTyrosine kinase domainC-cell diseaseC-cell pathologyLymph node metastasisCodon 918 mutationYounger median ageReceptor tyrosine kinase proteinTotal thyroidectomyProgressive diseaseMedian ageNode metastasisThyroid carcinomaType 2Germline mutationsTyrosine kinase proteinFormer groupTERT promoter mutations are rare in parathyroid tumors
Haglund F, Juhlin CC, Brown T, Ghaderi M, Liu T, Stenman A, Dinets A, Prasad M, Korah R, Xu D, Carling T, Larsson C. TERT promoter mutations are rare in parathyroid tumors. Endocrine Related Cancer 2015, 22: l9-l11. PMID: 25876648, DOI: 10.1530/erc-15-0121.Peer-Reviewed Original Research
2014
Morphology predicts BRAFV600E mutation in papillary thyroid carcinoma: an interobserver reproducibility study
Virk RK, Theoharis CG, Prasad A, Chhieng D, Prasad ML. Morphology predicts BRAFV600E mutation in papillary thyroid carcinoma: an interobserver reproducibility study. Virchows Archiv 2014, 464: 435-442. PMID: 24549591, DOI: 10.1007/s00428-014-1552-3.Peer-Reviewed Original Research
2013
Diagnostic SOX10 gene signatures in salivary adenoid cystic and breast basal-like carcinomas
Ivanov SV, Panaccione A, Nonaka D, Prasad ML, Boyd KL, Brown B, Guo Y, Sewell A, Yarbrough WG. Diagnostic SOX10 gene signatures in salivary adenoid cystic and breast basal-like carcinomas. British Journal Of Cancer 2013, 109: 444-451. PMID: 23799842, PMCID: PMC3721393, DOI: 10.1038/bjc.2013.326.Peer-Reviewed Original ResearchConceptsBasal-like breast carcinomasAdenoid cystic carcinomaGene signatureBreast cancerSox10 expressionDiagnostic markerBasal-like carcinomasExpression of ERPotential molecular markersSensitive diagnostic markerPotential therapeutic targetBasal cell markersNovel diagnostic markerSalivary adenoid cystic carcinomaMyoepithelial featuresBreast subtypesAdenoid cysticBreast carcinomaCystic carcinomaImmunohistochemical stainingMolecular subtypesHistological similaritiesTherapeutic targetTranscriptional program characteristicDiagnostic significance
2009
IMP3 is a novel biomarker for triple negative invasive mammary carcinoma associated with a more aggressive phenotype
Walter O, Prasad M, Lu S, Quinlan R, Edmiston K, Khan A. IMP3 is a novel biomarker for triple negative invasive mammary carcinoma associated with a more aggressive phenotype. Human Pathology 2009, 40: 1528-1533. PMID: 19695680, DOI: 10.1016/j.humpath.2009.05.005.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAged, 80 and overBiomarkers, TumorBreast NeoplasmsCarcinoma, Ductal, BreastDisease-Free SurvivalFemaleHumansImmunohistochemistryKaplan-Meier EstimateMiddle AgedNeoplasm ProteinsPhenotypeReceptor, ErbB-2Receptors, EstrogenReceptors, ProgesteroneRetrospective StudiesRNA-Binding ProteinsConceptsHuman epidermal growth factor receptor 2Invasive mammary carcinomaInvasive ductal carcinomaIMP3 expressionNovel biomarkersDuctal carcinomaProgesterone receptorMammary carcinomaBreast cancerEstrogen receptorAggressive phenotypeEpidermal growth factor receptor 2Triple-negative breast cancerDisease-free survivalBiologic prognostic factorsGrowth factor receptor 2Lymph node metastasisInsulin-like growth factor II mRNABasal-like carcinomasGrowth factor II mRNABasal epithelial markersNegative breast cancerBreast cancer casesFactor receptor 2Mouse monoclonal antibodyInsulin-Like Growth Factor mRNA Binding Protein 3 (IMP3) is Differentially Expressed in Benign and Malignant Follicular Patterned Thyroid Tumors
Slosar M, Vohra P, Prasad M, Fischer A, Quinlan R, Khan A. Insulin-Like Growth Factor mRNA Binding Protein 3 (IMP3) is Differentially Expressed in Benign and Malignant Follicular Patterned Thyroid Tumors. Endocrine Pathology 2009, 20: 149-157. PMID: 19449140, DOI: 10.1007/s12022-009-9079-x.Peer-Reviewed Original ResearchConceptsHürthle cell carcinomaPapillary thyroid carcinomaHürthle cell adenomaFollicular carcinomaGraves' diseaseHashimoto's thyroiditisColloid nodulesFollicular adenomaIMP3 expressionThyroid carcinomaStrong stainingInsulin-like growth factor mRNAThyroid lesionsProtein 3Conventional papillary thyroid carcinomaPathologic tumor characteristicsInsulin-like growth factor IIResidual thyroid tissueSurgical pathology archivesBinding protein 3Thyroid follicular lesionsGrowth factor IIGrowth factor mRNATumor characteristicsUterine cervix
2001
Gene expression in papillary thyroid carcinoma reveals highly consistent profiles
Huang Y, Prasad M, Lemon W, Hampel H, Wright F, Kornacker K, LiVolsi V, Frankel W, Kloos R, Eng C, Pellegata N, de la Chapelle A. Gene expression in papillary thyroid carcinoma reveals highly consistent profiles. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 15044-15049. PMID: 11752453, PMCID: PMC64980, DOI: 10.1073/pnas.251547398.Peer-Reviewed Original ResearchConceptsNumerous additional genesExtracellular matrix proteinsTranscriptase-PCRFunction-related proteinsAdditional genesSpecific molecular changesGene expressionDNA arraysExpression profilesTumor suppressorMatrix proteinsMolecular biologyGenesMolecular pathwaysReverse transcriptase-PCRPTC tissuesCITED1Molecular changesProteinODZ1Concordant expressionHigh expressionNormal thyroid tissueExpressionPapillary thyroid carcinomaExpression of Melanocytic Differentiation Markers in Malignant Melanomas of the Oral and Sinonasal Mucosa
Prasad M, Jungbluth A, Iversen K, Huvos A, Busam K. Expression of Melanocytic Differentiation Markers in Malignant Melanomas of the Oral and Sinonasal Mucosa. The American Journal Of Surgical Pathology 2001, 25: 782-787. PMID: 11395556, DOI: 10.1097/00000478-200106000-00010.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, NeoplasmDNA-Binding ProteinsGp100 Melanoma AntigenHumansMART-1 AntigenMelanocytesMelanomaMembrane GlycoproteinsMicrophthalmia-Associated Transcription FactorMonophenol MonooxygenaseMouth MucosaMouth NeoplasmsNasal MucosaNeoplasm ProteinsNose NeoplasmsS100 ProteinsTranscription FactorsConceptsHMB-45Mucosal melanomaMucosal tumorsSinonasal mucosaDesmoplastic melanomaImmunohistochemical studyMalignant melanomaOral mucosaSinonasal melanomaSensitive markerMART-1/MelanMelanocytic differentiation antigensOral mucosal tumorsOral mucosal melanomaSinonasal mucosal melanomaMelanocytic differentiation markersRoutine light microscopyNew monoclonal antibodySinonasal tractRare tumorFinal diagnosisMetastatic melanomaDifferentiation antigensMelanomaMucosa
2000
Are microinvasion and micrometastasis in breast cancer mountains or molehills?
Hoda S, Chiu A, Prasad M, Giri D, Hoda R. Are microinvasion and micrometastasis in breast cancer mountains or molehills? The American Journal Of Surgery 2000, 180: 305-308. PMID: 11113441, DOI: 10.1016/s0002-9610(00)00464-5.Commentaries, Editorials and LettersConceptsMicrometastatic carcinomaWidespread mammographic screeningSentinel lymph nodesDepartment of PathologyRate of diagnosisComputerized literature searchRate of detectionLymph nodesPathological examinationClinicopathological dataMicroinvasive carcinomaMammographic screeningClinical managementPathological diagnosisTreatment recommendationsBreast cancerDisease entityPubMed databaseMedical CollegeLiterature searchCarcinomaEarly detectionDiagnosisCumulative dataMicroinvasionMicroinvasive Carcinoma (T1mic) of the Breast
Prasad M, Osborne M, Giri D, Hoda S. Microinvasive Carcinoma (T1mic) of the Breast. The American Journal Of Surgical Pathology 2000, 24: 422-428. PMID: 10716157, DOI: 10.1097/00000478-200003000-00012.Peer-Reviewed Original ResearchConceptsDuct carcinomaMicroinvasive carcinomaPositive axillary lymphChest wall recurrenceFoci of invasionHigh-grade nucleiUnderwent lumpectomyAxillary lymphTNM criteriaClinicopathologic dataTubular carcinomaMean ageInvasive fociRadiation therapyAnticytokeratin antibodyEquivocal casesMammographic abnormalitiesPatientsRoutine examinationCarcinomaMuscle actinHistologic slidesMean numberOcular micrometerMICB