2023
Quantitative DNA Repair Biomarkers and Immune Profiling for Temozolomide and Olaparib in Metastatic Colorectal Cancer
Cecchini M, Zhang J, Wei W, Sklar J, Lacy J, Zhong M, Kong Y, Zhao H, DiPalermo J, Devine L, Stein S, Kortmansky J, Johung K, Bindra R, LoRusso P, Schalper K. Quantitative DNA Repair Biomarkers and Immune Profiling for Temozolomide and Olaparib in Metastatic Colorectal Cancer. Cancer Research Communications 2023, 3: 1132-1139. PMID: 37387791, PMCID: PMC10305782, DOI: 10.1158/2767-9764.crc-23-0045.Peer-Reviewed Original ResearchConceptsWhole-exome sequencingMGMT protein expressionColorectal cancerStable diseaseQuantitative immunofluorescenceT cellsProtein expressionPromoter hypermethylationLow MGMT protein expressionPARP inhibitorsRadiographic tumor regressionMetastatic colorectal cancerAdvanced colorectal cancerPretreatment tumor biopsiesEffector T cellsTumor-infiltrating lymphocytesMGMT proteinDNA repair biomarkersBaseline CD8Eligible patientsIncreased CD8Methylguanine-DNA methyltransferaseObjective responseProgressive diseaseImmune markers
2022
Metabolomic and transcriptomic signatures of influenza vaccine response in healthy young and older adults
Chou C, Mohanty S, Kang HA, Kong L, Avila‐Pacheco J, Joshi SR, Ueda I, Devine L, Raddassi K, Pierce K, Jeanfavre S, Bullock K, Meng H, Clish C, Santori FR, Shaw AC, Xavier RJ. Metabolomic and transcriptomic signatures of influenza vaccine response in healthy young and older adults. Aging Cell 2022, 21: e13682. PMID: 35996998, PMCID: PMC9470889, DOI: 10.1111/acel.13682.Peer-Reviewed Original ResearchConceptsInfluenza vaccine responsesInfluenza vaccinationVaccine responsesHigh respondersAntibody responseImmune responseMore effective influenza vaccinesOlder adultsEffective influenza vaccinesSevere respiratory infectionsRobust immune responseLow antibody responseInfluenza vaccineRespiratory infectionsSignificant morbiditySeasonal influenzaInflammatory responseAge-related differencesDay 28Flu seasonOlder subjectsVaccinationHR subjectsMetabolomic signaturePlasma metabolites
2008
Differential Expression of the Human CD8β Splice Variants and Regulation of the M-2 Isoform by Ubiquitination
Thakral D, Dobbins J, Devine L, Kavathas PB. Differential Expression of the Human CD8β Splice Variants and Regulation of the M-2 Isoform by Ubiquitination. The Journal Of Immunology 2008, 180: 7431-7442. PMID: 18490743, DOI: 10.4049/jimmunol.180.11.7431.Peer-Reviewed Original ResearchConceptsCytoplasmic tailSplice variantsExpression patternsCell surfaceDifferent cytoplasmic tailsGene splice variantsMRNA expression patternsMRNA levelsFluorescent chimerasHuman genesHuman lineageDifferential mRNA expression patternsHeterodimer functionsExtracellular domainLysine residuesQuantitative RT-PCRDifferential expressionLysosomal compartmentTCR complexFunctional significanceIsoformsCD8B geneUbiquitinationCell linesPrimary human
2006
Mapping the Binding Site on CD8β for MHC Class I Reveals Mutants with Enhanced Binding
Devine L, Thakral D, Nag S, Dobbins J, Hodsdon ME, Kavathas PB. Mapping the Binding Site on CD8β for MHC Class I Reveals Mutants with Enhanced Binding. The Journal Of Immunology 2006, 177: 3930-3938. PMID: 16951356, DOI: 10.4049/jimmunol.177.6.3930.Peer-Reviewed Original ResearchConceptsMHC class IClass IT cellsT cell responsesEffective immune responseT cell recognitionT cell hybridomasT cell membraneImmune responseCoreceptor functionCell responsesCell hybridomasInfected cellsCell recognitionIdentified mutationsCD8alphaCoreceptorCD8betaCDR3 loopsCellsEnhanced bindingCD8ImmunotherapyCD8alphabetaCD8β
2002
A Non-class I MHC Intestinal Epithelial Surface Glycoprotein, gp180, Binds to CD8
Campbell N, Park M, Toy L, Yio X, Devine L, Kavathas P, Mayer L. A Non-class I MHC Intestinal Epithelial Surface Glycoprotein, gp180, Binds to CD8. Clinical Immunology 2002, 102: 267-274. PMID: 11890713, DOI: 10.1006/clim.2001.5170.Peer-Reviewed Original ResearchMeSH KeywordsAbsorptionAdaptor Proteins, Signal TransducingAnti-Bacterial AgentsAntibodies, MonoclonalBlotting, WesternCarcinoma, HepatocellularCarrier ProteinsCD40 AntigensCD8-Positive T-LymphocytesEnterocytesEnzyme-Linked Immunosorbent AssayEpitopesExtracellular Matrix ProteinsHumansImmediate-Early ProteinsLiver NeoplasmsMembrane GlycoproteinsPhosphorylationProteinsRecombinant Fusion ProteinsSequestosome-1 ProteinTransfectionTumor Cells, CulturedTunicamycinConceptsActivation of CD8T cellsIntestinal epithelial cellsCD8 moleculesEpithelial cellsMucosal immune responsesClass I MHCNormal intestinal epithelial cellsT cell activationMurine T cellsCD8 alphaImmune responseCytolytic activityI MHCCell activationMixed cell culture systemCD8Human CD8 alphaMonoclonal antibodiesCell culture systemActivationSurface glycoproteinTyrosine kinaseCellsGp180
2000
Direct Detection and Magnetic Isolation of Chlamydia trachomatis Major Outer Membrane Protein-Specific CD8+ CTLs with HLA Class I Tetramers
Kim S, Devine L, Angevine M, DeMars R, Kavathas P. Direct Detection and Magnetic Isolation of Chlamydia trachomatis Major Outer Membrane Protein-Specific CD8+ CTLs with HLA Class I Tetramers. The Journal Of Immunology 2000, 165: 7285-7292. PMID: 11120863, DOI: 10.4049/jimmunol.165.12.7285.Peer-Reviewed Original ResearchMeSH KeywordsBacterial Outer Membrane ProteinsCells, CulturedChlamydia trachomatisCytotoxicity Tests, ImmunologicEpitopes, T-LymphocyteFemaleHematopoietic Stem CellsHLA-A2 AntigenHLA-B AntigensHumansImmunomagnetic SeparationLymphocyte CountLymphogranuloma VenereumMalePorinsProtein BindingT-Lymphocytes, CytotoxicConceptsHLA class ITetramer-binding T cellsCTL responsesT cellsPeripheral bloodClass IPeptide-specific T cellsHLA-A2 tetramersPeptide-pulsed targetsGenital tract infectionElicit CTL responsesMajor outer membrane proteinTetramer analysisTract infectionsPeptide stimulationImmunization trialsCytolytic activityChlamydia trachomatisUninfected individualsVivo inductionInfected individualsEpithelial cellsBloodBulk culturesStimulationHuman CD8β, But Not Mouse CD8β, Can Be Expressed in the Absence of CD8α as a ββ Homodimer
Devine L, Kieffer L, Aitken V, Kavathas P. Human CD8β, But Not Mouse CD8β, Can Be Expressed in the Absence of CD8α as a ββ Homodimer. The Journal Of Immunology 2000, 164: 833-838. PMID: 10623829, DOI: 10.4049/jimmunol.164.2.833.Peer-Reviewed Original ResearchConceptsChimeric proteinIg domainsCell-cell adhesion assaysBeta-beta dimersCD8 alphaForms of CD8CD8 betaTyrosine kinase p56lckCOS-7 cellsMHC class IDisulfide-linked homodimerCD8 alpha alpha homodimerCysteine residuesKinase p56lckCD8 alpha alphaAlpha alpha homodimerLack of expressionAlpha polypeptide chainPolypeptide chainMature T cellsCell surfaceClass IBeta-IgBeta betaAbility of CD8
1999
Molecular analysis of protein interactions mediating the function of the cell surface protein CD8
Devine L, Kavathas P. Molecular analysis of protein interactions mediating the function of the cell surface protein CD8. Immunologic Research 1999, 19: 201-210. PMID: 10493174, DOI: 10.1007/bf02786488.Peer-Reviewed Original ResearchConceptsProtein interactionsForms of CD8Such protein interactionsTyrosine kinase p56lckDisulfide-linked homodimerCell surface glycoproteinMolecular detailsKinase p56lckMutational analysisMolecular analysisFunctional differencesCoreceptor CD8ProteinHistocompatibility complex class IAffinity measurementsMajor histocompatibility complex class IComplex class ICellsP56lckHomodimerHeterodimersCrystal structureClass IGlycoproteinInteractionOrientation of the Ig domains of CD8 alpha beta relative to MHC class I.
Devine L, Sun J, Barr M, Kavathas P. Orientation of the Ig domains of CD8 alpha beta relative to MHC class I. The Journal Of Immunology 1999, 162: 846-51. PMID: 9916707, DOI: 10.4049/jimmunol.162.2.846.Peer-Reviewed Original Research