2022
An RNA-binding protein acts as a major post-transcriptional modulator in Bacillus anthracis
Pi H, Weiss A, Laut C, Grunenwald C, Lin H, Yi X, Stauff D, Skaar E. An RNA-binding protein acts as a major post-transcriptional modulator in Bacillus anthracis. Nature Communications 2022, 13: 1491. PMID: 35314695, PMCID: PMC8938561, DOI: 10.1038/s41467-022-29209-4.Peer-Reviewed Original ResearchConceptsHuman pathogen Bacillus anthracisRNA-binding proteinPost-transcriptional modulatorsPathogen Bacillus anthracisDetectable nuclease activityTwo-component systemBacillus anthracisGenetic competenceRNA turnoverDNA repairMRNA stabilityCellular metabolismNuclease activityEnvelope damageRNAProteinMultiple processesAnthracisHITRSporulationGenesMRNAExpressionMetabolismTurnover
2014
Structure and Cell Wall Cleavage by Modular Lytic Transglycosylase MltC of Escherichia coli
Artola-Recolons C, Lee M, Bernardo-García N, Blázquez B, Hesek D, Bartual S, Mahasenan K, Lastochkin E, Pi H, Boggess B, Meindl K, Usón I, Fisher J, Mobashery S, Hermoso J. Structure and Cell Wall Cleavage by Modular Lytic Transglycosylase MltC of Escherichia coli. ACS Chemical Biology 2014, 9: 2058-2066. PMID: 24988330, PMCID: PMC4168783, DOI: 10.1021/cb500439c.Peer-Reviewed Original ResearchConceptsTwo-domain structureLytic transglycosylasesCell wall assemblyEscherichia coliEssential bacterial enzymeResolution X-ray structureÅ resolution X-ray structureGram-negative bacterium Escherichia coliBacterium Escherichia coliBacterial cell wallCell wall analoguesBacterial genomesGlycan strandsGenomic analysisActive siteCell wallBacterial enzymesFunctional roleSoluble constructWall assemblyGenomeTransglycosylasesExolytic cleavageX-ray structurePeptidoglycanDiscovery of a New Class of Non-β-lactam Inhibitors of Penicillin-Binding Proteins with Gram-Positive Antibacterial Activity
O’Daniel P, Peng Z, Pi H, Testero S, Ding D, Spink E, Leemans E, Boudreau M, Yamaguchi T, Schroeder V, Wolter W, Llarrull L, Song W, Lastochkin E, Kumarasiri M, Antunes N, Espahbodi M, Lichtenwalter K, Suckow M, Vakulenko S, Mobashery S, Chang M. Discovery of a New Class of Non-β-lactam Inhibitors of Penicillin-Binding Proteins with Gram-Positive Antibacterial Activity. Journal Of The American Chemical Society 2014, 136: 3664-3672. PMID: 24517363, PMCID: PMC3985699, DOI: 10.1021/ja500053x.Peer-Reviewed Original ResearchConceptsMethicillin-resistant Staphylococcus aureusPenicillin-binding protein 2aLinezolid-resistant methicillin-resistant Staphylococcus aureusNon-β-lactam antibioticsGlobal public health concernSerious global public health concernPublic health concernClasses of antibioticsMouse modelVivo efficacyOral bioavailabilityProtein 2ABactericidal activityGram-positive antibacterial activityStaphylococcus aureusNon-β-lactam inhibitorsInfectionPenicillin binding proteinsAntibioticsAntibacterial activityVancomycin
2013
Reactions of All Escherichia coli Lytic Transglycosylases with Bacterial Cell Wall
Lee M, Hesek D, Llarrull L, Lastochkin E, Pi H, Boggess B, Mobashery S. Reactions of All Escherichia coli Lytic Transglycosylases with Bacterial Cell Wall. Journal Of The American Chemical Society 2013, 135: 3311-3314. PMID: 23421439, PMCID: PMC3645847, DOI: 10.1021/ja309036q.Peer-Reviewed Original Research