Cell-Extracellular Matrix Interactions Play Multiple Essential Roles in Aortic Arch Development
Warkala M, Chen D, Ramirez A, Jubran A, Schonning M, Wang X, Zhao H, Astrof S. Cell-Extracellular Matrix Interactions Play Multiple Essential Roles in Aortic Arch Development. Circulation Research 2020, 128: e27-e44. PMID: 33249995, PMCID: PMC7864893, DOI: 10.1161/circresaha.120.318200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAorta, ThoracicCell LineageCell-Matrix JunctionsEndothelial Progenitor CellsExtracellular MatrixExtracellular Matrix ProteinsFibronectinsGene Expression Regulation, DevelopmentalGestational AgeIntegrin alpha5beta1LIM-Homeodomain ProteinsMice, 129 StrainMice, Inbred C57BLMice, KnockoutMorphogenesisSignal TransductionTranscription FactorsConceptsSecond heart fieldPharyngeal arch arteriesCell-ECM interactionsPAA formationAortic arch arteriesPharyngeal archesArch arteriesIntegrin α5β1Whole-mount confocal imagingEssential roleCell-extracellular matrix interactionsFourth pharyngeal arch arteryMultiple essential rolesEndothelial cell dynamicsNeural crest-derived cellsCrest-derived cellsLethal birth defectEC progenitorsUnderstanding genesHeart fieldLineage tracingVascular smooth muscle cellsMorphogenesisAortic arch developmentDevelopmental stagesEmerging roles of PLCγ1 in endothelial biology
Chen D, Simons M. Emerging roles of PLCγ1 in endothelial biology. Science Signaling 2021, 14 PMID: 34344833, PMCID: PMC8507396, DOI: 10.1126/scisignal.abc6612.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiologyEndothelial CellsHumansPhospholipase C gammaPhosphorylationSignal TransductionConceptsUnique protein structurePhospholipase C-γ1Receptor tyrosine kinasesRole of PLCγ1Major physiological roleMolecular functionsDistinct vascular phenotypePLC familyC-γ1Signal transducerProtein structureDirect effectorTyrosine kinaseMembrane lipidsSecond messengerFunction mutationsPhysiological rolePLCγ1Endothelial biologyEndothelial cancerCritical roleVascular phenotypeEndothelial cellsKinaseFuture investigationsFibronectin signals through integrin α5β1 to regulate cardiovascular development in a cell type-specific manner
Chen D, Wang X, Liang D, Gordon J, Mittal A, Manley N, Degenhardt K, Astrof S. Fibronectin signals through integrin α5β1 to regulate cardiovascular development in a cell type-specific manner. Developmental Biology 2015, 407: 195-210. PMID: 26434918, PMCID: PMC5312697, DOI: 10.1016/j.ydbio.2015.09.016.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBranchial RegionCardiovascular SystemCell LineageEmbryo, MammalianFemaleFibronectinsIntegrin alpha5beta1LIM-Homeodomain ProteinsMice, KnockoutModels, BiologicalMorphogenesisMutationNeural CrestOrgan SpecificityPharynxPhenotypePregnancySignal TransductionStem CellsT-Box Domain ProteinsThymus GlandTranscription FactorsConceptsPharyngeal arch arteriesCardiovascular developmentIntegrin α5Pharyngeal arch mesodermCell type-specific mannerCell typesMid-gestation lethalityType-specific mannerDistinct cell typesCardiac outflow tractMorphogenetic defectsPharyngeal regionMouse embryogenesisConditional mutantsExtracellular matrix glycoproteinEmbryonic developmentMutagenesis studiesCardiovascular morphogenesisNeural crestPAA formationSurface ectodermDefective formationArch arteriesFN1Matrix glycoprotein