2020
Repurposing FDA-approved drugs for SARS-CoV-2 through an ELISA-based screening for the inhibition of RBD/ACE2 interaction
Fu W, Chen Y, Wang K, Hettinghouse A, Hu W, Wang J, Lei Z, Chen Z, Stapleford K, Liu C. Repurposing FDA-approved drugs for SARS-CoV-2 through an ELISA-based screening for the inhibition of RBD/ACE2 interaction. Protein & Cell 2020, 12: 586-591. PMID: 33210243, PMCID: PMC7673315, DOI: 10.1007/s13238-020-00803-w.Peer-Reviewed Original ResearchAngiotensin-Converting Enzyme 2COVID-19Drug RepositioningEnzyme-Linked Immunosorbent AssayHumansPharmaceutical PreparationsSARS-CoV-2In Vitro Physical and Functional Interaction Assays to Examine the Binding of Progranulin Derivative Atsttrin to TNFR2 and Its Anti-TNFα Activity
Fu W, Hettinghouse A, Liu C. In Vitro Physical and Functional Interaction Assays to Examine the Binding of Progranulin Derivative Atsttrin to TNFR2 and Its Anti-TNFα Activity. Methods In Molecular Biology 2020, 2248: 109-119. PMID: 33185871, PMCID: PMC8112733, DOI: 10.1007/978-1-0716-1130-2_8.Peer-Reviewed Original ResearchMeSH KeywordsCells, CulturedEnzyme-Linked Immunosorbent AssayOsteogenesisProgranulinsProtein BindingProtein Interaction MappingReceptors, Tumor Necrosis Factor, Type IIStructure-Activity RelationshipTumor Necrosis Factor-alphaConceptsAnti-TNFα activityAutoimmune diseasesTartrate-resistant acid phosphatase (TRAP) stainingAnti-TNFα therapyCollagen-induced arthritisInflammatory disease modelsGood therapeutic effectAcid phosphatase stainingGrowth factor-like moleculesTNF inhibitorsTherapeutic effectTNFα activityProgranulinFunctional inhibitionTNFR2AtsttrinDisease modelsPhosphatase stainingTNFRTNFαDiseaseInhibitionCritical roleDirect bindingHigh affinity
2018
Role of ADAMTS‐12 in Protecting Against Inflammatory Arthritis in Mice By Interacting With and Inactivating Proinflammatory Connective Tissue Growth Factor
Wei J, Fu W, Hettinghouse A, He W, Lipson K, Liu C. Role of ADAMTS‐12 in Protecting Against Inflammatory Arthritis in Mice By Interacting With and Inactivating Proinflammatory Connective Tissue Growth Factor. Arthritis & Rheumatology 2018, 70: 1745-1756. PMID: 29750395, PMCID: PMC6203634, DOI: 10.1002/art.40552.Peer-Reviewed Original ResearchConceptsConnective tissue growth factorCIA mouse modelADAMTS-12Inflammatory arthritisTissue growth factorMouse modelCollagen-induced arthritis modelGrowth factorRheumatoid arthritis developmentEnzyme-linked immunosorbentRA patientsArthritis developmentPannus formationSerum levelsCartilage destructionArthritis modelInflammatory cytokinesEnhanced osteoclastogenesisHealthy subjectsArthritisAnkle jointTomography scanningInflammationSusceptibility genesCritical regulator