2018
The Germline-Specific Factor OEF-1 Facilitates Coordinated Progression Through Germ Cell Development in Caenorhabditis elegans
McManus CE, Reinke V. The Germline-Specific Factor OEF-1 Facilitates Coordinated Progression Through Germ Cell Development in Caenorhabditis elegans. Genetics 2018, 208: 549-563. PMID: 29167199, PMCID: PMC5788521, DOI: 10.1534/genetics.117.1123.Peer-Reviewed Original ResearchConceptsGerm cell developmentGerm cellsCell developmentGermline-expressed genesNovel nuclear proteinCell cycle checkpointsGermline expressionGenomic integrityUnique fateOocyte differentiationFaithful transmissionCycle checkpointsNuclear proteinsMature gametesX chromosomeDevelopmental eventsLarval stagesMaturation eventsGenetic materialCaenorhabditisFactor 1Developmental progressionSubtle defectsCellsExpression
2011
C. elegans meg‐1 and meg‐2 differentially interact with nanos family members to either promote or inhibit germ cell proliferation and survival
Kapelle WS, Reinke V. C. elegans meg‐1 and meg‐2 differentially interact with nanos family members to either promote or inhibit germ cell proliferation and survival. Genesis 2011, 49: 380-391. PMID: 21305687, DOI: 10.1002/dvg.20726.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedCaenorhabditis elegansCaenorhabditis elegans ProteinsCell ProliferationCell SurvivalDisorders of Sex DevelopmentEmbryo, NonmammalianEpistasis, GeneticFemaleGene Expression Regulation, DevelopmentalGerm CellsLuminescent ProteinsMaleRNA InterferenceRNA-Binding ProteinsTime FactorsConceptsGerm cell proliferationMeg 1Germ cellsEmbryonic primordial germ cellsTargeted RNAi screenPrimordial germ cellsCell proliferationGerm cell survivalGerm lineageP granulesRNAi screenLarval stagesMEG 2Cell survivalFamily membersMultiple pathwaysGerm cell degenerationPhenotypeSterilityProliferationOptimal proliferationCellsCell degenerationEmbryogenesisLineagesThe kinase VRK1 is required for normal meiotic progression in mammalian oogenesis
Schober CS, Aydiner F, Booth CJ, Seli E, Reinke V. The kinase VRK1 is required for normal meiotic progression in mammalian oogenesis. Cells And Development 2011, 128: 178-190. PMID: 21277975, DOI: 10.1016/j.mod.2011.01.004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsChromosomes, MammalianFemaleHistonesInfertility, FemaleInfertility, MaleMaleMeiosisMiceMice, Inbred C57BLMice, Mutant StrainsMutagenesis, InsertionalOocytesOogenesisOrgan SizeOrgan SpecificityOvaryPhenotypePhosphorylationProtein Serine-Threonine KinasesSeminiferous EpitheliumSpermatogenesisTestisTumor Suppressor Protein p53ConceptsMeiotic progressionNormal meiotic progressionGene trap insertionConserved roleDrosophila oogenesisMammalian gametogenesisMammalian oogenesisVRK1 activityPhosphorylation substratesFemale meiosisInvertebrate speciesProliferation defectMale spermatogoniaChromosomal configurationsMetaphase plateVRK1OogenesisVRK1 expressionFailure of oocytesMouse strainsDrosophilaMeiosisGametogenesisChromosomesLoci
2009
DPL-1 (DP) acts in the germ line to coordinate ovulation and fertilization in C. elegans
Chi W, Reinke V. DPL-1 (DP) acts in the germ line to coordinate ovulation and fertilization in C. elegans. Cells And Development 2009, 126: 406-416. PMID: 19368797, PMCID: PMC2680456, DOI: 10.1016/j.mod.2009.01.008.Peer-Reviewed Original ResearchConceptsEFL-1/DPLDpl-1Germ lineTissue-specific rescue experimentsGonad armsItr-1RME-2Caenorhabditis elegansEfl-1C. elegansGene functionTranscriptional programsInositol triphosphate receptorGonad structureSuccessful reproductionSevere defectsRescue experimentsGerm cellsFunction mutationsSuccessful fertilizationLikely disruptsMutantsSpermathecaTriphosphate receptorElegans
2008
C. elegans Nucleostemin Is Required for Larval Growth and Germline Stem Cell Division
Kudron MM, Reinke V. C. elegans Nucleostemin Is Required for Larval Growth and Germline Stem Cell Division. PLOS Genetics 2008, 4: e1000181. PMID: 18725931, PMCID: PMC2515194, DOI: 10.1371/journal.pgen.1000181.Peer-Reviewed Original ResearchConceptsRibosome biogenesisGermline stem cell divisionLarval arrest phenotypeGerm line functionGermline stem cellsStem cell divisionCell growthNematode C. elegansN-terminal domainStem cellsExhibit reduced levelsCell cycle arrestArrest phenotypeNucleolar factorsC. elegansRRNA transcriptionGrowth defectNucleolar functionGerm lineCell divisionLarval growthTransgenic studiesBiogenesisStable expressionProliferative stateA C. elegans Piwi, PRG-1, Regulates 21U-RNAs during Spermatogenesis
Wang G, Reinke V. A C. elegans Piwi, PRG-1, Regulates 21U-RNAs during Spermatogenesis. Current Biology 2008, 18: 861-867. PMID: 18501605, PMCID: PMC2494713, DOI: 10.1016/j.cub.2008.05.009.Peer-Reviewed Original ResearchConceptsPRG-1Small RNAsC. elegansGerm cellsSubset of mRNAsPRG 2Cell totipotencyPIWI proteinsP granulesRibonucleoprotein granulesEpigenetic regulationSpecific family membersGerm linePiwi familyProper expressionPiwiSuccessful spermatogenesisDiverse classPiRNAsRNAElegansProteinSpermatogenesisExpressionFamily members
2002
X-chromosome silencing in the germline of C. elegans.
Kelly WG, Schaner CE, Dernburg AF, Lee MH, Kim SK, Villeneuve AM, Reinke V. X-chromosome silencing in the germline of C. elegans. Development 2002, 129: 479-92. PMID: 11807039, PMCID: PMC4066729, DOI: 10.1242/dev.129.2.479.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaenorhabditis elegansDisorders of Sex DevelopmentFemaleGene Expression Regulation, DevelopmentalGene SilencingGenes, ReporterGerm CellsGonadsHistonesImmunohistochemistryIn Situ Hybridization, FluorescenceMaleMeiosisMethylationOligonucleotide Array Sequence AnalysisOocytesRecombinant Fusion ProteinsSpermatozoaTranscription, GeneticTransgenesX ChromosomeConceptsX chromosomeHistone modificationsC. elegansEarly meiosisX chromosome silencingNematode C. elegansGermline maintenanceNematode phylumHermaphroditic speciesChromatin organizationSex bodyXO malesTranscriptional competenceSex chromosomesTranscriptional activationTransgene arrayOocyte meiosisMeiosisChromosomesRepressive mechanismsElegansSpeciesHeterochromatinHermaphroditesGenome
2000
Genome-wide analysis of developmental and sex-regulated gene expression profiles in Caenorhabditis elegans
Jiang M, Ryu J, Kiraly M, Duke K, Reinke V, Kim S. Genome-wide analysis of developmental and sex-regulated gene expression profiles in Caenorhabditis elegans. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 98: 218-223. PMID: 11134517, PMCID: PMC14571, DOI: 10.1073/pnas.98.1.218.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsCaenorhabditis elegansCyclinsDisorders of Sex DevelopmentFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalGenes, HelminthGenes, RetinoblastomaGenomeGenomicsMaleOligonucleotide Array Sequence AnalysisProto-Oncogene ProteinsSex CharacteristicsSex DifferentiationSignal TransductionTranscription FactorsWnt ProteinsZebrafish ProteinsConceptsDNA microarraysCaenorhabditis elegansFull-genome DNA microarraysGene expressionSex-specific differentiationGenome-wide analysisGene expression profilesDifferent developmental stagesComplete molecular profileGene expression levelsExpression profilesExpression dataGenesDevelopmental stagesMolecular levelWorm strainsElegansExpression levelsMicroarrayGrowth conditionsRelative levelsMolecular profileExpressionGlobal viewHermaphroditesA Global Profile of Germline Gene Expression in C. elegans
Reinke V, Smith H, Nance J, Wang J, Van Doren C, Begley R, Jones S, Davis E, Scherer S, Ward S, Kim S. A Global Profile of Germline Gene Expression in C. elegans. Molecular Cell 2000, 6: 605-616. PMID: 11030340, DOI: 10.1016/s1097-2765(00)00059-9.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArgonaute ProteinsCaenorhabditis elegansCaenorhabditis elegans ProteinsCell Cycle ProteinsCytoskeletal ProteinsDrosophila ProteinsGene Expression ProfilingGene Expression Regulation, DevelopmentalHelminth ProteinsMaleMembrane ProteinsOocytesProteinsReceptors, NotchRNA, MessengerRNA-Induced Silencing ComplexSpermatogenesisSpermatozoaX ChromosomeConceptsC. elegans germlineGermline gene expressionEmbryonic signaling pathwaysGene expression patternsStem cell proliferationChromosomal locationProtein kinaseX chromosomeDNA microarraysExpression patternsGene expressionSignaling pathwaysGermline transcriptsGenesCell proliferationGermlineGlobal profileTranscriptsChromosomesNew componentsKinaseMicroarrayPhosphatasePathwayOocytes