2017
Gender difference in kidney electrolyte transport. I. Role of AT1a receptor in thiazide-sensitive Na+-Cl− cotransporter activity and expression in male and female mice
Li J, Hatano R, Xu S, Wan L, Yang L, Weinstein AM, Palmer L, Wang T. Gender difference in kidney electrolyte transport. I. Role of AT1a receptor in thiazide-sensitive Na+-Cl− cotransporter activity and expression in male and female mice. American Journal Of Physiology. Renal Physiology 2017, 313: f505-f513. PMID: 28566500, PMCID: PMC5582908, DOI: 10.1152/ajprenal.00087.2017.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IIAnimalsDiuresisFemaleHydrochlorothiazideKidneyMaleMice, KnockoutNatriuresisPhenotypeProtein Serine-Threonine KinasesReceptor, Angiotensin, Type 1Receptors, DrugSex CharacteristicsSodium Chloride SymportersSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSolute Carrier Family 12, Member 3ConceptsUrine volumeKO miceCotransporter activityRenal clearance experimentsReceptor knockout miceGlomerular filtration rateBolus intravenous injectionDelivery of NaClNCC protein expressionGender differencesFiltration rateFemale miceAT1A receptorClearance experimentsKO animalsIntravenous injectionNCC expressionDistal nephronFractional NaKnockout miceMale KOProximal tubulesHCTZMiceNHE3 expression
2008
Neutrophil gelatinase-associated lipocalin suppresses cyst growth by Pkd1 null cells in vitro and in vivo
Wei F, Karihaloo A, Yu Z, Marlier A, Seth P, Shibazaki S, Wang T, Sukhatme VP, Somlo S, Cantley LG. Neutrophil gelatinase-associated lipocalin suppresses cyst growth by Pkd1 null cells in vitro and in vivo. Kidney International 2008, 74: 1310-1318. PMID: 18974761, PMCID: PMC3793389, DOI: 10.1038/ki.2008.395.Peer-Reviewed Original ResearchConceptsNeutrophil Gelatinase-Associated LipocalinCyst growthGelatinase-associated lipocalinAutosomal dominant polycystic kidney diseaseTubular cell proliferationDominant polycystic kidney diseaseCell linesPolycystic kidney diseasePkd1 mutant miceRenal functionAdenoviral delivery systemAssociated lipocalinKidney diseaseRate of proliferationCyst enlargementProliferative responseRenal epithelial cellsProximal tubulesMutant miceEpithelial cellsFlow stimuliCell proliferationDecreased rateApical ciliaProliferation
2003
Expression of ENaC and serum- and glucocorticoid-induced kinase 1 in the rat intestinal epithelium
Coric T, Hernandez N, de la Rosa D, Shao D, Wang T, Canessa CM. Expression of ENaC and serum- and glucocorticoid-induced kinase 1 in the rat intestinal epithelium. AJP Gastrointestinal And Liver Physiology 2003, 286: g663-g670. PMID: 14630642, DOI: 10.1152/ajpgi.00364.2003.Peer-Reviewed Original ResearchMeSH KeywordsAldosteroneAnimalsAntibodies, BlockingAntibody SpecificityBlotting, NorthernCHO CellsColonCricetinaeDNA, ComplementaryEnzyme InductionEpithelial Sodium ChannelsEpitheliumFluorescent Antibody TechniqueImmediate-Early ProteinsIntestinal MucosaNuclear ProteinsPlasmidsProtein Serine-Threonine KinasesRatsRNA, MessengerSignal TransductionSodium ChannelsConceptsDistal colonGlucocorticoid-induced kinase 1Intestinal epitheliumConcentrations of aldosteroneSubunits of ENaCSodium channel activityExpression of ENaCRat intestinal epitheliumKinase 1Aldosterone levelsAldosterone-dependent mannerSingle doseSodium depletionChronic increaseDistal tubulesControl animalsAldosteroneAldosterone inductionRat intestineBasal conditionsColonSodium transportersEpitheliumSites of expressionSGK1Distribution and regulation of expression of serum‐ and glucocorticoid‐induced kinase‐1 in the rat kidney
de la Rosa D, Coric T, Todorovic N, Shao D, Wang T, Canessa C. Distribution and regulation of expression of serum‐ and glucocorticoid‐induced kinase‐1 in the rat kidney. The Journal Of Physiology 2003, 551: 455-466. PMID: 12816971, PMCID: PMC2343216, DOI: 10.1113/jphysiol.2003.042903.Peer-Reviewed Original ResearchMeSH KeywordsAdrenalectomyAldosteroneAnimalsAntibodiesAntibody SpecificityBlotting, NorthernBlotting, WesternCells, CulturedDNA, ComplementaryElectrophoresis, Polyacrylamide GelEpithelial CellsGene Expression Regulation, EnzymologicGlucocorticoidsImmediate-Early ProteinsImmunoblottingIn Vitro TechniquesIsoenzymesKidneyKidney TubulesMicroscopy, FluorescenceNuclear ProteinsProtein Serine-Threonine KinasesRatsRats, Sprague-DawleyRNASubcellular FractionsTransfectionConceptsGlucocorticoid-induced kinase 1Kinase 1Ion channelsRegulation of expressionConstitutive high expressionBasolateral membraneRenal epithelial cellsSubcellular localizationLevel of expressionRegulation of levelsEpithelial ion channelsSGK1 proteinMammalian kidneyApical membraneDirect interactionSGK1Epithelial cellsWestern blottingHigh expressionExpressionExpression of SGK1ProteinRat kidneyTransportersPhysiological changes