2024
Rationale and Design of a Phase 2, Double-blind, Placebo-Controlled, Randomized Trial Evaluating AMP Kinase-Activation by Metformin in Focal Segmental Glomerulosclerosis
Barsotti G, Luciano R, Kumar A, Meliambro K, Kakade V, Tokita J, Naik A, Fu J, Peck E, Pell J, Reghuvaran A, Tanvir E, Patel P, Zhang W, Li F, Moeckel G, Perincheri S, Cantley L, Moledina D, Wilson F, He J, Menon M. Rationale and Design of a Phase 2, Double-blind, Placebo-Controlled, Randomized Trial Evaluating AMP Kinase-Activation by Metformin in Focal Segmental Glomerulosclerosis. Kidney International Reports 2024, 9: 1354-1368. PMID: 38707807, PMCID: PMC11068976, DOI: 10.1016/j.ekir.2024.02.006.Peer-Reviewed Original ResearchMinimal change diseaseRandomized controlled trialsSafety of metforminDouble-blindPodocyte injuryAdjunctive therapyPlacebo-controlled randomized controlled trialsPhase III studyPhase II trialPrimary glomerular diseaseFocal segmental glomerulosclerosisEffect of metforminPhase IIPlacebo-controlledPreclinical dataNovel urineChange diseaseTissue markersRandomized trialsSegmental glomerulosclerosisGlomerular diseaseMechanistic biomarkersObservational studyFSGSInexpensive agent
2023
Profilin1 is required to prevent mitotic catastrophe in murine and human glomerular diseases
Tian X, Pedigo C, Li K, Ma X, Bunda P, Pell J, Lek A, Gu J, Zhang Y, Rangel P, Li W, Schwartze E, Nagata S, Lerner G, Perincheri S, Priyadarshini A, Zhao H, Lek M, Menon M, Fu R, Ishibe S. Profilin1 is required to prevent mitotic catastrophe in murine and human glomerular diseases. Journal Of Clinical Investigation 2023, 133: e171237. PMID: 37847555, PMCID: PMC10721156, DOI: 10.1172/jci171237.Peer-Reviewed Original ResearchConceptsProteinuric kidney diseaseKidney diseasePodocyte lossHuman glomerular diseasesMitotic catastrophePodocyte cell cycleSevere proteinuriaCell cycle reentryKidney failureGlomerular diseaseCell cycleKidney tissueG1/S checkpointUnsuccessful repairCyclin D1Glomerular integrityIrregular nucleiTissue-specific lossMouse podocytesPodocytesAltered expressionDiseaseCyclin B1Ribosome affinity purificationMultinucleated cellsRecent Advances in Genitourinary Tumors: Updates From the 5th Edition of the World Health Organization Blue Book Series.
Riddle N, Parkash V, Guo C, Shen S, Perincheri S, Ramirez A, Auerbach A, Belchis D, Humphrey P. Recent Advances in Genitourinary Tumors: Updates From the 5th Edition of the World Health Organization Blue Book Series. Archives Of Pathology & Laboratory Medicine 2023, 148: 952-964. PMID: 38031818, DOI: 10.5858/arpa.2022-0509-ra.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsMale genital tumorsWorld Health Organization classificationGenital tumorsOrganization classificationClinical practiceDate clinical practiceRoutine clinical practiceWorld Health OrganizationUrinary tumorsGenitourinary tumorsSimilar tumorsDiagnostic criteriaTumor seriesTumorsGenetic syndromesOrgan systemsHealth OrganizationComprehensive updateUrinaryNew entitySyndromeGenomic terminologySeverity of Coexisting Diabetic Glomerular Lesions Affects Outcomes in Primary Glomerular Disease
Perincheri S. Severity of Coexisting Diabetic Glomerular Lesions Affects Outcomes in Primary Glomerular Disease. Kidney360 2023, 4: 1345-1346. PMID: 37883998, PMCID: PMC10617791, DOI: 10.34067/kid.0000000000000267.Commentaries, Editorials and LettersA Rare Case of Methotrexate-Associated Lymphoproliferative Disease in the Orbit
Spadaro J, Perzia B, Perincheri S, Habib L. A Rare Case of Methotrexate-Associated Lymphoproliferative Disease in the Orbit. Ophthalmic Plastic And Reconstructive Surgery 2023, 40: e28-e31. PMID: 37791841, DOI: 10.1097/iop.0000000000002528.Peer-Reviewed Case Reports and Technical NotesRheumatoid arthritisLymphoproliferative diseaseIatrogenic immunodeficiency-associated lymphoproliferative disordersImmunodeficiency-associated lymphoproliferative disordersRight upper eyelid ptosisDiscontinuation of methotrexateRemission 18 monthsAtypical lymphoid infiltrateT-cell markersUpper eyelid ptosisSuperior orbital massMedication withdrawalLymphoid infiltratesIncisional biopsyLymphoproliferative disordersLow proliferation rateComplete resolutionEyelid ptosisPathologic analysisMonoclonal proliferationOrbital massRare caseCaucasian femaleClose monitoringRearrangement studiesIRF8 may be a useful marker for blastic plasmacytoid dendritic cell neoplasm, especially with weak CD123 expression
Tang H, Panse G, Braddock D, Perincheri S, Xu M, McNiff J. IRF8 may be a useful marker for blastic plasmacytoid dendritic cell neoplasm, especially with weak CD123 expression. Journal Of Cutaneous Pathology 2023, 50: 595-600. PMID: 37082914, DOI: 10.1111/cup.14439.Peer-Reviewed Original ResearchConceptsBlastic plasmacytoid dendritic cell neoplasmPlasmacytoid dendritic cell neoplasmDendritic cell neoplasmPunch biopsy specimenBiopsy specimenCell neoplasmsCase of BPDCNUseful markerTumor cellsTCL-1 expressionAtypical mononuclear cellsBone marrow involvementDiffuse dermal infiltrateDendritic cell lineagePotential diagnostic pitfallRegulatory factor 8TCL-1BPDCN casesCD123 expressionMarrow involvementDermal infiltrateCutaneous nodulesMyelodysplastic syndromeSkin nodulesMononuclear cellsDownregulation of a tumor suppressor gene LKB1 in lung transplantation as a biomarker for chronic murine lung allograft rejection
Rahman M, Ravichandran R, Sankpal N, Bansal S, Sureshbabu A, Fleming T, Perincheri S, Bharat A, Smith M, Bremner R, Mohanakumar T. Downregulation of a tumor suppressor gene LKB1 in lung transplantation as a biomarker for chronic murine lung allograft rejection. Cellular Immunology 2023, 386: 104690. PMID: 36812767, PMCID: PMC11019891, DOI: 10.1016/j.cellimm.2023.104690.Peer-Reviewed Original ResearchConceptsLung allograft rejectionLung transplantationAllograft rejectionChronic lung allograft rejectionBronchiolitis obliterans syndromeLung transplant recipientsTumor suppressor gene LKB1Phosphorylated mammalian targetBEAS-2B cellsObliterans syndromeChronic rejectionTransplant recipientsDonor lungsSingle lungB6D2F1 miceMurine modelDBA/2J miceMTOR expressionMammalian targetLKB1 overexpressionSignificant downregulationLungLKB1 knockdownA549 cellsLKB1 activity“Double‐hit” ineffective erythropoiesis—concurrent β‐thalassemia with α‐gene triplication and myelodysplastic syndrome with SF3B1 mutation
Chen P, Gorshein E, Tormey C, Siddon A, Perincheri S. “Double‐hit” ineffective erythropoiesis—concurrent β‐thalassemia with α‐gene triplication and myelodysplastic syndrome with SF3B1 mutation. American Journal Of Hematology 2023, 98: 984-988. PMID: 36688500, DOI: 10.1002/ajh.26856.Peer-Reviewed Educational Materials
2022
Cell Cycle and Senescence Regulation by Podocyte Histone Deacetylase 1 and 2
Rangel P, Cross E, Liu C, Pedigo C, Tian X, Gutiérrez-Calabrés E, Nagata S, Priyadarshini A, Lerner G, Bunda P, Perincheri S, Gu J, Zhao H, Wang Y, Inoue K, Ishibe S. Cell Cycle and Senescence Regulation by Podocyte Histone Deacetylase 1 and 2. Journal Of The American Society Of Nephrology 2022, 34: 433-450. PMID: 36414418, PMCID: PMC10103311, DOI: 10.1681/asn.2022050598.Peer-Reviewed Original ResearchConceptsCell cycle entryDNA damageSenescence-associated β-galactosidase activityDouble knockout miceRole of HDACsNormal glomerular filtration barrierAssociated phenotypesP21-mediated cell cycle arrestOpen chromatin conformationGlomerular filtration barrierSevere proteinuriaKidney failureProinflammatory cytokinesCell cycle regulationHistone deacetylase 1Cell cycle arrestKi67 expressionSustained DNA damagePodocyte lossIntact expressionMice leadsPodocyte-specific lossMatrix metalloproteinasesPodocyte detachmentProteinuriaLung Transplant Recipients with SARS-CoV-2 Infection Induce Circulating Exosomes with SARS-CoV-2 Spike Protein S2 Which Are Immunogenic in Mice
Bansal S, Fleming T, Perincheri S, Smith M, Bremner R, Mohanakumar T. Lung Transplant Recipients with SARS-CoV-2 Infection Induce Circulating Exosomes with SARS-CoV-2 Spike Protein S2 Which Are Immunogenic in Mice. The Journal Of Heart And Lung Transplantation 2022, 41: s134. PMCID: PMC8988572, DOI: 10.1016/j.healun.2022.01.314.Peer-Reviewed Original ResearchSARS-CoV-2 spike proteinSARS-CoV-2SARS-CoV-2 infectionSARS-CoV-2 spike antigenLung transplant recipientsSpike proteinTransplant recipientsSpike antigenNucleocapsid antigenSARS-CoV-2 infection inducesSevere acute respiratory syndrome coronavirus 2Acute respiratory syndrome coronavirus 2Respiratory syndrome coronavirus 2Conclusion SARS-CoV-2Lungs of miceSyndrome coronavirus 2Important risk factorSARS-CoV-2 spikeSpike protein antigensC57BL/6 miceCoronavirus 2Severe inflammationInfection inducesRisk factorsViral antigens
2021
Chronic Lung Allograft Dysfunction: Role for Tumor Suppressor Gene Liver Kinase B1
Rahman M, Ravichandran R, Bansal S, Liu W, Perincheri S, Angara S, Fleming T, Bharat A, Hachem R, Smith M, Bremner R, Mohanakumar T. Chronic Lung Allograft Dysfunction: Role for Tumor Suppressor Gene Liver Kinase B1. The Journal Of Heart And Lung Transplantation 2021, 40: s60. DOI: 10.1016/j.healun.2021.01.1886.Peer-Reviewed Original ResearchChronic lung allograft dysfunctionBronchiolitis obliterans syndromeLung transplant modelLung transplant recipientsLiver kinase B1Epithelial-mesenchymal transformationΑ-SMAChronic rejectionTransplant recipientsBEAS-2BTransplant modelHypoxia/reperfusion (H/R) modelKnockdown of LKB1Lung allograft dysfunctionQRT-PCRAirway epithelial cell lineMiR-155 levelsExosome releaseHypoxia/reperfusionRole of LKB1Upregulation of vimentinAllograft dysfunctionLung transplantationObliterans syndromeCLAD development
2009
The HDAC Inhibitor TSA Inhibits Cell Proliferation, Induces Apoptosis and Down-Regulates HER2 Protein and Gene Expression as a Single Agent and in Combination with Trastuzumab in Trastuzumab-Sensitive and -Resistant Breast Cancer Cell Lines.
Radke S, Perincheri S, Schulz V, Lerner B, Kumar A, Chandrasekan L, Tuck D, Harris L. The HDAC Inhibitor TSA Inhibits Cell Proliferation, Induces Apoptosis and Down-Regulates HER2 Protein and Gene Expression as a Single Agent and in Combination with Trastuzumab in Trastuzumab-Sensitive and -Resistant Breast Cancer Cell Lines. Cancer Research 2009, 69: 3133-3133. DOI: 10.1158/0008-5472.sabcs-09-3133.Peer-Reviewed Original ResearchRole of HDACiHistone deacetylase inhibitorsTrastuzumab-resistant breast cancerBreast cancerBreast cancer cell linesHER2 proteinCancer cell linesSingle agentResistant breast cancer cell linesCell linesHER2-positive breast cancerFirst biological therapyHER2 antibody trastuzumabValue of HER2Positive breast cancerTSA treatmentNormal breast epitheliumNormal breast cell lineAlternative therapeutic strategiesBreast cancer cellsGrowth inhibitory effectsInhibited cell survivalHDAC inhibitor trichostatinResistant cell linesMechanism of action