Sean Pennetti, MD

Research Fellow, Yale University May – August 2016

The role of Granulin, a TLR-9 protein cofactor, and cytidine deaminase deficiency in the development of autoimmune diabetes in NOD mice

Dr. Li Wen M.D., Primary Investigator

• Performed intraocular exsanguination, oral gavage, urinalysis and glucose analysis on murine models.
• Extracted Pancreatic, Mesenteric lymph nodes, as well as whole pancreas and Peyer’s patch samples.
• Performed PCR, qPCR, flow cytometry, Western Blot, and DNA analysis.

Research Assistant, University of Pittsburgh September 2014 – July 2015

Uridine 5’-Triphosphate (UTP) as a propagator of arteriogenesis in response to femoral artery occlusion

Dr. Ryan McEnaney M.D., Primary Investigator

• Performed femoral artery ligations, employed LDPI, and pressure testing within a murine model.
• Fluent in IHC, IF, H&E staining, cell culture, confocal microscopy, and Western Blotting techniques
• Regularly performed tissue preparation employing cryostat and formalin fixation techniques

Research Assistant, University of Pittsburgh January – June 2014

Prostate specific membrane antigen (PSMA) in prostate cancer pathogenesis and as a potential novel marker

Drs. Denise O’Keefe Ph.D. and Dean Bacich Ph.D., Primary Investigators

• Analyzed the effects of folic acid supplementation on tumor proliferation within a murine model.
• Employed the use of both in vivo knockout murine models and in vitro tumor colonies.
• Responsible for maintaining and genotyping a murine breeding program.
• Routinely preformed both Classical and RT-PCR analyses of cell lines and study mice
• Isolated RNA and performed cDNA transformation of murine genetic materials.

Research Assistant, Tulane University October 2011– May 2012

The potential benefits of stem cell therapy in Krabbe disease, globoid cell leukodystrophy

Dr. Bruce Bunnell Ph.D., Primary Investigator

• Conducted movement and behavior analysis in murine models.
• Performed PCR analysis and cell culture work.

Research Assistant, Penn State University August – December 2010

The confounding effects of white coat hypertension and patient anxiety in accurately assessing blood pressure.

Dr. William Gerin Ph.D., Primary investigator

• monitored blood pressure and heart rate fluctuations employing 24-hr holter monitoring.
• conducted patient interviews and performed data analysis.