2021
Excessive adventitial stress drives inflammation-mediated fibrosis in hypertensive aortic remodelling in mice
Spronck B, Latorre M, Wang M, Mehta S, Caulk AW, Ren P, Ramachandra AB, Murtada SI, Rojas A, He CS, Jiang B, Bersi MR, Tellides G, Humphrey JD. Excessive adventitial stress drives inflammation-mediated fibrosis in hypertensive aortic remodelling in mice. Journal Of The Royal Society Interface 2021, 18: 20210336. PMID: 34314650, PMCID: PMC8315831, DOI: 10.1098/rsif.2021.0336.Peer-Reviewed Original ResearchConceptsAortic remodellingWall stressAngiotensin II infusionInflammatory cell infiltrationC57BL/6 J miceSmooth muscle contractile processHigh wall stressVasoconstrictive capacityII infusionBlood pressureInflammatory cellsCell infiltrationMean wall stressJ miceThoracic aortaMaladaptive remodellingImmune processesAortaBulk RNA sequencingBiomechanical assessmentAdaptive remodellingContractile processMarked increaseRemodellingVasoconstriction
2018
Single-cell RNA sequencing reveals microglia-like cells in cerebrospinal fluid during virologically suppressed HIV
Farhadian SF, Mehta SS, Zografou C, Robertson K, Price RW, Pappalardo J, Chiarella J, Hafler DA, Spudich SS. Single-cell RNA sequencing reveals microglia-like cells in cerebrospinal fluid during virologically suppressed HIV. JCI Insight 2018, 3: e121718. PMID: 30232286, PMCID: PMC6237230, DOI: 10.1172/jci.insight.121718.Peer-Reviewed Original ResearchConceptsCerebrospinal fluidHIV infectionImmune activationAntiretroviral therapyNeuronal injuryCentral nervous system immune activationLong-term suppressive antiretroviral therapySingle-cell RNA sequencingCNS immune activationDisease-associated microgliaSuppressive antiretroviral therapyImmune cell subsetsMicroglia-like cellsGene expression signaturesNeuronal damageNeuroinflammatory diseasesRNA sequencingCell subsetsCNS cellsNeurological conditionsRare subsetNeurocognitive impairmentMyeloid cellsCellular subsetsInfection