2020
Repeat tick exposure elicits distinct immune responses in guinea pigs and mice
Kurokawa C, Narasimhan S, Vidyarthi A, Booth CJ, Mehta S, Meister L, Diktas H, Strank N, Lynn GE, DePonte K, Craft J, Fikrig E. Repeat tick exposure elicits distinct immune responses in guinea pigs and mice. Ticks And Tick-borne Diseases 2020, 11: 101529. PMID: 32993942, PMCID: PMC7530331, DOI: 10.1016/j.ttbdis.2020.101529.Peer-Reviewed Original ResearchConceptsGuinea pigsElicit distinct immune responsesDistinct immune responsesGuinea pig modelLocal blood flowImmune animalsInflammatory pathwaysTick rejectionMechanisms of resistanceImmune responseMouse modelVaccine candidatesBite siteBlood flowPig modelCoagulation pathwayComplement activationAcquired ResistanceProtective antigenTick detachmentTick proteinsBlood mealMiceTick infestationRNA sequencing
2019
Prefrontal cortex interneurons display dynamic sex-specific stress-induced transcriptomes
Girgenti MJ, Wohleb ES, Mehta S, Ghosal S, Fogaca MV, Duman RS. Prefrontal cortex interneurons display dynamic sex-specific stress-induced transcriptomes. Translational Psychiatry 2019, 9: 292. PMID: 31712551, PMCID: PMC6848179, DOI: 10.1038/s41398-019-0642-z.Peer-Reviewed Original ResearchConceptsFluorescence-activated cell sortingInitiation factor 2Distinct transcriptome profilesTranslational machineryTranscriptome profilesEnriched pathwaysTranscriptional pathwaysTranscriptional profilesRNA sequencingDepressive-like behaviorSST interneuronsKey pathwaysChronic stressInterneuron subtypesSex-specific differencesPrefrontal cortexCell sortingSignificant dysregulationFactor 2PathwayStress-induced depressive-like behaviorDecreased expressionReporter miceGrowth factorNon-stressed females
2018
Single-cell RNA sequencing reveals microglia-like cells in cerebrospinal fluid during virologically suppressed HIV
Farhadian SF, Mehta SS, Zografou C, Robertson K, Price RW, Pappalardo J, Chiarella J, Hafler DA, Spudich SS. Single-cell RNA sequencing reveals microglia-like cells in cerebrospinal fluid during virologically suppressed HIV. JCI Insight 2018, 3: e121718. PMID: 30232286, PMCID: PMC6237230, DOI: 10.1172/jci.insight.121718.Peer-Reviewed Original ResearchConceptsCerebrospinal fluidHIV infectionImmune activationAntiretroviral therapyNeuronal injuryCentral nervous system immune activationLong-term suppressive antiretroviral therapySingle-cell RNA sequencingCNS immune activationDisease-associated microgliaSuppressive antiretroviral therapyImmune cell subsetsMicroglia-like cellsGene expression signaturesNeuronal damageNeuroinflammatory diseasesRNA sequencingCell subsetsCNS cellsNeurological conditionsRare subsetNeurocognitive impairmentMyeloid cellsCellular subsetsInfection
2016
Loss of the SUMO protease Ulp2 triggers a specific multichromosome aneuploidy
Ryu HY, Wilson NR, Mehta S, Hwang SS, Hochstrasser M. Loss of the SUMO protease Ulp2 triggers a specific multichromosome aneuploidy. Genes & Development 2016, 30: 1881-1894. PMID: 27585592, PMCID: PMC5024685, DOI: 10.1101/gad.282194.116.Peer-Reviewed Original ResearchConceptsSUMO protease Ulp2Small ubiquitin-related modifierPost-translational protein modificationWhole-genome RNA sequencingNumerous cellular pathwaysG1 phase cyclinsGenome maintenanceChromosome IUlp2Particular chromosomeAdapts cellsCell divisionSUMO proteaseCellular stressRNA sequencingProtein modificationCellular pathwaysNormal copy numberTranscript levelsMost human tumorsCopy numberRapid adaptationExtra copiesChromosomesSpecific aneuploidies