2008
Development of Immunogenic Tumor-Loaded Dendritic Cells Through Physical Perturbation and Apoptotic Cell Loading
Shen X, Berger CL, Tigelaar R, Edelson RL. Development of Immunogenic Tumor-Loaded Dendritic Cells Through Physical Perturbation and Apoptotic Cell Loading. Immunological Investigations 2008, 37: 798-821. PMID: 18991097, DOI: 10.1080/08820130802403358.Peer-Reviewed Original ResearchMeSH KeywordsAntigen PresentationAntigens, CDAntigens, NeoplasmApoptosisB7-1 AntigenCancer VaccinesCD8-Positive T-LymphocytesCell DifferentiationCell Line, TumorCytotoxicity, ImmunologicDendritic CellsHumansImmunoglobulinsLymphocyte ActivationLymphoma, T-CellMechanotransduction, CellularMembrane GlycoproteinsMonocytesPerforinReceptors, CCR7Silicon DioxideConceptsT cellsDendritic cellsAllogeneic T cell proliferationCD8 T cellsLevels of perforinChemokine receptor CCR7T cell proliferationApoptotic tumor cellsUniverse of antigensDC vaccinesImmunogenic tumorsImmature DCsReceptor CCR7TNF-alphaPassage of leukocytesIFN-gammaHuman monocytesMonocytesTumor cellsPotent inductionCell apoptosisPhenotypic conversionCell proliferationApoptotic cellsCells induces
2001
Induction of human tumor‐loaded dendritic cells
Berger C, Xu A, Hanlon D, Lee C, Schechner J, Glusac E, Christensen I, Snyder E, Holloway V, Tigelaar R, Edelson R. Induction of human tumor‐loaded dendritic cells. International Journal Of Cancer 2001, 91: 438-447. PMID: 11251964, DOI: 10.1002/1097-0215(200002)9999:9999<::aid-ijc1073>3.0.co;2-r.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CDApoptosisCD36 AntigensCell DifferentiationCell DivisionCell SurvivalCells, CulturedCytokinesCytoplasmDendritic CellsDose-Response Relationship, DrugFlow CytometryHumansImmunoglobulinsImmunohistochemistryImmunophenotypingImmunotherapyLeukapheresisLeukocytesLymphocytesMajor Histocompatibility ComplexMembrane GlycoproteinsMonocytesPhagocytosisPhenotypeTime FactorsT-LymphocytesConceptsExtracorporeal photopheresisDistinctive antigensTumor-loaded dendritic cellsStandard extracorporeal photopheresisMHC class II moleculesImproved cancer immunotherapyDendritic cell differentiationAnti-cancer vaccinesMalignant T cellsClass II moleculesTransition of monocytesDC vaccinesMalignant cell apoptosisDendritic cellsImmature DCsCancer immunotherapyCD83 expressionT cellsExogenous cytokinesSignificant proliferationCell apoptosisVaccineMembrane CD36Cell differentiationCells
2000
Development of Dendritic Epidermal T Cells with a Skewed Diversity of γδTCRs in Vδ1-Deficient Mice
Hara H, Kishihara K, Matsuzaki G, Takimoto H, Tsukiyama T, Tigelaar R, Nomoto K. Development of Dendritic Epidermal T Cells with a Skewed Diversity of γδTCRs in Vδ1-Deficient Mice. The Journal Of Immunology 2000, 165: 3695-3705. PMID: 11034374, DOI: 10.4049/jimmunol.165.7.3695.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalCell DifferentiationClone CellsCytokinesDendritic CellsEmbryonic and Fetal DevelopmentEpidermal CellsEpidermisFemaleFibroblast Growth Factor 10Fibroblast Growth Factor 7Fibroblast Growth FactorsGene DeletionGene Rearrangement, delta-Chain T-Cell Antigen ReceptorGenes, T-Cell Receptor deltaGenetic MarkersGrowth SubstancesImmunophenotypingMaleMiceMice, Inbred C57BLMice, KnockoutProtein ConformationReceptors, Antigen, T-Cell, gamma-deltaStem CellsThymus GlandT-LymphocytesEnterocyte Expression of Interleukin 7 Induces Development of γδ T Cells and Peyer's Patches
Laky K, Lefrançois L, Lingenheld E, Ishikawa H, Lewis J, Olson S, Suzuki K, Tigelaar R, Puddington L. Enterocyte Expression of Interleukin 7 Induces Development of γδ T Cells and Peyer's Patches. Journal Of Experimental Medicine 2000, 191: 1569-1580. PMID: 10790431, PMCID: PMC2213426, DOI: 10.1084/jem.191.9.1569.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarrier ProteinsDendritic CellsEnterocytesEpidermal CellsEpidermisFatty Acid-Binding Protein 7Fatty Acid-Binding ProteinsGerminal CenterInterleukin-7Intestine, SmallMiceMice, TransgenicMyelin P2 ProteinNeoplasm ProteinsNerve Tissue ProteinsPeyer's PatchesReceptors, Antigen, T-Cell, gamma-deltaRecombinant Fusion ProteinsTissue DistributionT-LymphocytesTransgenesConceptsTCR-gammadelta cellsIntraepithelial lymphocytesT cell developmentCell developmentPeyer's patchesIL-7Extrathymic T cell developmentΓδ T cellsMucosal lymphoid tissuesGammadelta intraepithelial lymphocytesIL-7 expressionT cell receptorB cell maturationIntestinal fatty acidExtrathymic developmentLymphoid tissueT cellsIntestinal mucosaEnterocyte expressionBone marrowProtein promoterIntestinal epitheliumCell maturationCell receptorMice
1998
Conservation of T Cell Receptor Conformation in Epidermal γδ Cells with Disrupted Primary Vγ Gene Usage
Mallick-Wood C, Lewis J, Richie L, Owen M, Tigelaar R, Hayday A. Conservation of T Cell Receptor Conformation in Epidermal γδ Cells with Disrupted Primary Vγ Gene Usage. Science 1998, 279: 1729-1733. PMID: 9497293, DOI: 10.1126/science.279.5357.1729.Peer-Reviewed Original ResearchConceptsGene segmentsVariable region gene segmentsRegion gene segmentsParticular gene segmentsReceptor conformationTCR chainsReceptor gammaAssociation of expressionAlphabeta T cellsCellsConservationT-cell receptor gammaConformational determinantsGammadelta T cell subsetsGene usageT cellsConformationΓδ cellsB cellsLymphocyte repertoireVgamma5SitesDendritic epidermal T cellsExpressionRepertoire
1995
Thy‐1 + dendritic cells express truncated form of POMC mRNA
Farooqui J, Medrano E, Boissy R, Tigelaar R, Nordlund J. Thy‐1 + dendritic cells express truncated form of POMC mRNA. Experimental Dermatology 1995, 4: 297-301. PMID: 8589920, DOI: 10.1111/j.1600-0625.1995.tb00208.x.Peer-Reviewed Original ResearchConceptsDendritic cellsThy-1Anterior pituitary tumor cell lineACTH precursor proopiomelanocortinEpidermal Thy-1Expression of proopiomelanocortinNorthern blot analysisBlot analysisPituitary tumor cell lineDerivative peptidesMucocutaneous disordersThymic lymphocytesPOMC peptidesPrecursor proopiomelanocortinTumor cell linesPOMC mRNAProopiomelanocortinPigmentary anomaliesPositive controlACTHCell linesLymphocytesAtT20 cellsPolyclonal antibodiesTotal RNAImmunobiology of Mouse Dendritic Epidermal T Cells: A Decade Later, Some Answers, But Still More Questions
Tigelaar R, Lewis J. Immunobiology of Mouse Dendritic Epidermal T Cells: A Decade Later, Some Answers, But Still More Questions. Journal Of Investigative Dermatology 1995, 105: s43-s49. DOI: 10.1038/jid.1995.9.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsDendritic epidermal T cellsEpidermal T cellsT cell receptorΓδ cellsT cellsMouse dendritic epidermal T-cellsMurine dendritic epidermal T cellsCutaneous immune surveillanceNormal human skinSelf antigensImmune surveillanceNormal miceActive hair growthAdult miceEpithelial sitesCutaneous physiologyReproductive tractTissue restrictionHair growthFunctional activityMiceSkinHuman skinReceptorsOverwhelming evidenceImmunobiology of mouse dendritic epidermal T cells: a decade later, some answers, but still more questions.
Tigelaar R, Lewis J. Immunobiology of mouse dendritic epidermal T cells: a decade later, some answers, but still more questions. Journal Of Investigative Dermatology 1995, 105: 43s-49s. PMID: 7615996, DOI: 10.1111/1523-1747.ep12315280.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsDendritic epidermal T cellsEpidermal T cellsGamma delta cellsT cell receptorT cellsMouse dendritic epidermal T-cellsMurine dendritic epidermal T cellsDelta T-cell receptorCutaneous immune surveillanceDelta cellsAlpha beta cellsNormal human skinSelf antigensImmune surveillanceNormal miceActive hair growthAdult miceBeta cellsEpithelial sitesCutaneous physiologyReproductive tractTissue restrictionHair growthFunctional activityMiceRegulation of Transgenic Class II Major Histocompatibility Genes in Murine Langerhans Cells
Longley J, Ding T, Levin D, Lewis J, Edelson R, Tigelaar R, Flavell R. Regulation of Transgenic Class II Major Histocompatibility Genes in Murine Langerhans Cells. Journal Of Investigative Dermatology 1995, 104: 329-334. PMID: 7860996, DOI: 10.1111/1523-1747.ep12665365.Peer-Reviewed Original ResearchConceptsSplenic dendritic cellsEpidermal Langerhans cellsLangerhans cellsDendritic cellsE expressionClass II major histocompatibility complex moleculesLangerhans cells expressionMurine Langerhans cellsMajor histocompatibility complex moleculesNear-normal levelsB6 genetic backgroundHistocompatibility complex moleculesMajor histocompatibility complex class II gene expressionImmune functionClass II proteinsImmunoperoxidase stainingCell-specific regulatory sequencesClass II gene expressionTransgenic miceCell expressionImmunofluorescence cytometryIntact animalsDecrease expressionMajor histocompatibility genesHistocompatibility genes
1992
T Lymphocytes Bearing Gamma-Delta Antigen Receptors in Skin
Modlin R, Lewis J, Uyemura K, Tigelaar R. T Lymphocytes Bearing Gamma-Delta Antigen Receptors in Skin. Chemical Immunology And Allergy 1992, 53: 61-74. PMID: 1534236, DOI: 10.1159/000319103.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
1990
TCR γ/δ+ Dendritic Epidermal T Cells as Constituents of Skin-Associated Lymphoid Tissue
Tigelaar R, Lewis J, Bergstresser P. TCR γ/δ+ Dendritic Epidermal T Cells as Constituents of Skin-Associated Lymphoid Tissue. Journal Of Investigative Dermatology 1990, 94: s58-s63. PMID: 1972173, DOI: 10.1111/1523-1747.ep12875138.Peer-Reviewed Original ResearchConceptsDendritic epidermal T cellsGamma/delta cellsT cell antigen receptorEpidermal T cellsT cellsCD4-CD8Alpha/beta T cell antigen receptorGamma/delta typeSkin-Associated Lymphoid TissueBone marrow-derived cellsGamma/delta populationsClass II MHC moleculesDelta cellsMarrow-derived cellsTCR junctional regionsEarly fetal thymocytesMHC-like moleculesLangerhans cellsLymphoid tissueTCR heterogeneityNormal miceMHC moleculesThy-1Fetal thymocytesMHC type
1989
Limited diversity of T-cell receptor gamma-chain expression of murine Thy-1+ dendritic epidermal cells revealed by V gamma 3-specific monoclonal antibody.
Havran W, Grell S, Duwe G, Kimura J, Wilson A, Kruisbeek A, O'Brien R, Born W, Tigelaar R, Allison J. Limited diversity of T-cell receptor gamma-chain expression of murine Thy-1+ dendritic epidermal cells revealed by V gamma 3-specific monoclonal antibody. Proceedings Of The National Academy Of Sciences Of The United States Of America 1989, 86: 4185-4189. PMID: 2726770, PMCID: PMC287415, DOI: 10.1073/pnas.86.11.4185.Peer-Reviewed Original ResearchPhenotypic and functional analysis of gamma delta T cell receptor-positive murine dendritic epidermal clones.
Havran W, Poenie M, Tigelaar R, Tsien R, Allison J. Phenotypic and functional analysis of gamma delta T cell receptor-positive murine dendritic epidermal clones. The Journal Of Immunology 1989, 142: 1422-8. PMID: 2783942, DOI: 10.4049/jimmunol.142.5.1422.Peer-Reviewed Original ResearchConceptsIL-2R expressionThy-1TCR complexSecretion of lymphokinesRelease of lymphokinesGamma delta TCRIntracellular free calciumEffect of antibodiesFunctional TCR complexDendritic cellsLy-6CPharmacologic agentsNormal miceT cellsCytolytic activityDelta TCRFree calciumGamma deltaAlpha betaDEC cellsCD3 complexEpidermal clonesProtein kinase CActivation signalsLymphokines
1988
Limited diversity of γδ antigen receptor genes of thy-1+ dendritic epidermal cells
Asarnow D, Kuziel W, Bonyhad M, Tigelaar R, Tucker P, Allison J. Limited diversity of γδ antigen receptor genes of thy-1+ dendritic epidermal cells. Cell 1988, 55: 837-847. PMID: 2847872, DOI: 10.1016/0092-8674(88)90139-0.Peer-Reviewed Original ResearchConceptsT cellsGamma delta antigen receptorsDendritic epidermal cellsPeripheral lymphoid tissuesAdult T-cellEarly fetal thymusLymphoid tissueImmune surveillanceFetal thymusFetal thymocytesThy-1Antigen receptorCell clonesAlpha betaReceptor geneAntigen receptor genesMinor populationDelta gene segmentsReceptorsMajor populationCellsDelta geneStriking homogeneityGene segmentsCertain epitheliaγδ Antigen receptors of Thy-1 + dendritic epidermal cells: Implications for thymic differentiation
Allison J, Hayran W, Asarnow D, Tigelaar R, Tucker P, Bonyhadi M. γδ Antigen receptors of Thy-1 + dendritic epidermal cells: Implications for thymic differentiation. Immunologic Research 1988, 7: 292. PMID: 2906352, DOI: 10.1007/bf02935534.Peer-Reviewed Original ResearchAnimalsAntigens, Differentiation, T-LymphocyteAntigens, SurfaceBase SequenceCD3 ComplexCell DifferentiationDendritic CellsDNAGene Expression RegulationGene Rearrangement, gamma-Chain T-Cell Antigen ReceptorGene Rearrangement, T-LymphocyteMiceReceptors, Antigen, T-CellReceptors, Antigen, T-Cell, gamma-deltaThy-1 AntigensT-LymphocytesEffect of Keratinocyte Cytokines on Thy‐1+ Dendritic Epidermal Cells
TIGELAAR R, NIXON‐FULTON J, TAKASHIMA A, KUZIEL W, TAKIJIRI C, LEWIS J, TUCKER P, BERGSTRESSER P. Effect of Keratinocyte Cytokines on Thy‐1+ Dendritic Epidermal Cells. Annals Of The New York Academy Of Sciences 1988, 548: 271-282. PMID: 2470301, DOI: 10.1111/j.1749-6632.1988.tb18815.x.Peer-Reviewed Original ResearchThy-1+ epidermal cells in nude mice are distinct from their counterparts in thymus-bearing mice. A study of morphology, function, and T cell receptor expression.
Nixon-Fulton J, Kuziel W, Santerse B, Bergstresser P, Tucker P, Tigelaar R. Thy-1+ epidermal cells in nude mice are distinct from their counterparts in thymus-bearing mice. A study of morphology, function, and T cell receptor expression. The Journal Of Immunology 1988, 141: 1897-903. PMID: 2902136, DOI: 10.4049/jimmunol.141.6.1897.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, SurfaceBlotting, NorthernBlotting, SouthernCell DivisionConcanavalin ADendritic CellsEpidermal CellsEpidermisEthersFemaleInterleukin-2IonomycinMaleMiceMice, Inbred BALB CMice, Inbred CBAMice, NudeReceptors, Antigen, T-CellSpecies SpecificityTetradecanoylphorbol AcetateThy-1 AntigensThymus GlandConceptsIL-2Thy-1Nude miceNormal miceLow-dose IL-2BALB/c miceT cell receptor expressionModest proliferative responseThymus-bearing miceCell receptor expressionT cell differentiationTCR-gamma transcriptsC miceReceptor expressionAthymic miceProliferative responseNormal animalsTCR mRNACalcium ionophoreEpidermal cellsMiceNormal CBACon AFollicular epitheliumPopulation of cellsThy-1+ Dendritic Epidermal Cells in Mice: Precursor Frequency Analysis and Cloning of Concanavalin A-Reactive Cells
Takashima A, Nixon-Fulton J, Bergstresser P, Tigelaar R. Thy-1+ Dendritic Epidermal Cells in Mice: Precursor Frequency Analysis and Cloning of Concanavalin A-Reactive Cells. Journal Of Investigative Dermatology 1988, 90: 671-678. PMID: 2896217, DOI: 10.1111/1523-1747.ep12560835.Peer-Reviewed Original ResearchConceptsDendritic epidermal cellsPrecursor frequency analysisIL-2Lyt-2Thy-1Con AGrowth factorExogenous IL-2Higher proliferative responsesFunctional activityCytotoxic activityYAC-1Precursor frequencyProliferative responseMouse Thy-1Dilution microculturesLow cytotoxic activityHigh cytotoxic activityTarget cellsLow responsivenessCells/Vivo activityBulk culturesFiller cellsEpidermal cells
1987
Regulation of T-cell receptor γ-chain RNA expression in murine Thy-1+ dendritic epidermal cells
Kuziel W, Takashima A, Bonyhadi M, Bergstresser P, Allison J, Tigelaar R, Tucker P. Regulation of T-cell receptor γ-chain RNA expression in murine Thy-1+ dendritic epidermal cells. Nature 1987, 328: 263-266. PMID: 2885757, DOI: 10.1038/328263a0.Peer-Reviewed Original ResearchConceptsEpidermal cellsT cell receptorDendritic epidermal cellsThy-1Steady-state levelsMurine Thy-1T lymphocytesCell differentiationT-cell lineageT cell differentiationDistinct populationsMost T lymphocytesRNA expressionMajor histocompatibility complexΔ complexNecessary eventΑ-chainΓ-chainMinor populationDendritic cellsLangerhans cellsExpressionCellsNormal miceHistocompatibility complexLack of dendritic Thy-1+ epidermal cells in mice with severe combined immunodeficiency disease.
Nixon-Fulton J, Witte P, Tigelaar R, Bergstresser P, Kumar V. Lack of dendritic Thy-1+ epidermal cells in mice with severe combined immunodeficiency disease. The Journal Of Immunology 1987, 138: 2902-5. PMID: 2883236, DOI: 10.4049/jimmunol.138.9.2902.Peer-Reviewed Original ResearchConceptsDendritic Thy-1SCID miceEpidermal cell suspensionsThy-1NK cellsT lymphocytesEpidermal whole mountsSevere combined immunodeficiency diseaseCombined immunodeficiency diseaseNormal bone marrowControl miceImmunodeficiency diseaseBone marrowWhole mountsMiceImmunofluorescent stainingCon ACell suspensionsEC suspensionsEpidermal cellsLymphocytesPrecursor levelsCellsTransplantationMarrow