2022
ArhGEF12 activates Rap1A and not RhoA in human dermal microvascular endothelial cells to reduce tumor necrosis factor‐induced leak
Khan A, Ni W, Baltazar T, Lopez‐Giraldez F, Pober JS, Pierce RW. ArhGEF12 activates Rap1A and not RhoA in human dermal microvascular endothelial cells to reduce tumor necrosis factor‐induced leak. The FASEB Journal 2022, 36: e22254. PMID: 35294066, PMCID: PMC9103844, DOI: 10.1096/fj.202101873rr.Peer-Reviewed Original Research
2021
Tumor necrosis factor‐induced ArhGEF10 selectively activates RhoB contributing to human microvascular endothelial cell tight junction disruption
Khan A, Ni W, Lopez‐Giraldez F, Kluger MS, Pober JS, Pierce RW. Tumor necrosis factor‐induced ArhGEF10 selectively activates RhoB contributing to human microvascular endothelial cell tight junction disruption. The FASEB Journal 2021, 35: e21627. PMID: 33948992, PMCID: PMC9026622, DOI: 10.1096/fj.202002783rr.Peer-Reviewed Original ResearchConceptsCapillary endothelial cellsHuman dermal microvascular endothelial cellsMicrovascular endothelial cellsEndothelial cellsTight junctionsCultured human microvascular endothelial cellsEC tight junctionsLoss of barrierCapillary leak syndromeCapillary barrier functionDermal microvascular endothelial cellsRhoB activationTight junction disruptionDisrupts tight junctionsHuman microvascular endothelial cellsExtent of TNFHuman capillary endothelial cellsLeak syndromeOverwhelming inflammationCapillary leakBarrier lossTJ disruptionJunction disruptionRhoB knockdownTNF
2016
IL-17 Promotes Neutrophil-Mediated Immunity by Activating Microvascular Pericytes and Not Endothelium
Liu R, Lauridsen HM, Amezquita RA, Pierce RW, Jane-Wit D, Fang C, Pellowe AS, Kirkiles-Smith NC, Gonzalez AL, Pober JS. IL-17 Promotes Neutrophil-Mediated Immunity by Activating Microvascular Pericytes and Not Endothelium. The Journal Of Immunology 2016, 197: 2400-2408. PMID: 27534549, PMCID: PMC5010945, DOI: 10.4049/jimmunol.1600138.Peer-Reviewed Original ResearchMeSH KeywordsCaspase 9Cells, CulturedCulture MediaCytokinesEndothelium, VascularGranulocyte Colony-Stimulating FactorGranulocyte-Macrophage Colony-Stimulating FactorHumansInterleukin-17Neutrophil InfiltrationNeutrophilsPericytesReceptors, Interleukin-17Sequence Analysis, RNATumor Necrosis Factor-alphaVenulesConceptsIL-17Endothelial cellsIL-17RAIL-17RC subunitsCultured human endothelial cellsInflammatory gene expressionMicrovascular endothelial cellsNeutrophil-mediated immunityNeutrophil infiltrationNeutrophilic inflammationHuman endothelial cellsAcute inflammationIL-1βNeutrophil functionProinflammatory cytokinesIL-8Proinflammatory moleculesIL-1αIL-17RCIL-1Human pericytesG-CSFMicrovascular pericytesNeutrophil productionNeutrophil survival