Featured Publications
Histone Acetyltransferases p300 and CBP Coordinate Distinct Chromatin Remodeling Programs in Vascular Smooth Muscle Plasticity
Chakraborty R, Ostriker AC, Xie Y, Dave JM, Gamez-Mendez A, Chatterjee P, Abu Y, Valentine J, Lezon-Geyda K, Greif DM, Schulz VP, Gallagher PG, Sessa WC, Hwa J, Martin KA. Histone Acetyltransferases p300 and CBP Coordinate Distinct Chromatin Remodeling Programs in Vascular Smooth Muscle Plasticity. Circulation 2022, 145: 1720-1737. PMID: 35502657, DOI: 10.1161/circulationaha.121.057599.Peer-Reviewed Original ResearchConceptsHistone acetylationContractile genesContractile protein expressionPhenotypic switchingHistone acetyl transferase p300Human intimal hyperplasiaPlatelet-derived growth factor treatmentAcetyl transferase p300Key regulatory mechanismSmooth muscle cell phenotypeP300 expressionP300-dependent acetylationSmooth muscle plasticityDistinct functional interactionsMuscle cell phenotypeProtein expressionIntimal hyperplasiaRole of p300Methylcytosine dioxygenase TET2Chromatin modificationsEpigenetic regulationVSMC phenotypic switchingSpecific histoneCardiovascular diseaseMaster regulatorTET2 Protects Against Vascular Smooth Muscle Cell Apoptosis and Intimal Thickening in Transplant Vasculopathy
Ostriker AC, Xie Y, Chakraborty R, Sizer AJ, Bai Y, Ding M, Song WL, Huttner A, Hwa J, Martin KA. TET2 Protects Against Vascular Smooth Muscle Cell Apoptosis and Intimal Thickening in Transplant Vasculopathy. Circulation 2021, 144: 455-470. PMID: 34111946, PMCID: PMC8643133, DOI: 10.1161/circulationaha.120.050553.Peer-Reviewed Original ResearchMeSH KeywordsAllograftsAnimalsApoptosisBiomarkersDioxygenasesDisease Models, AnimalDisease SusceptibilityDNA-Binding ProteinsHeart TransplantationHumansImmunohistochemistryInterferon-gammaMiceMice, KnockoutMyocytes, Smooth MuscleSignal TransductionSTAT1 Transcription FactorTunica IntimaVascular DiseasesConceptsCoronary allograft vasculopathyGraft arteriopathyIntimal thickeningCAV progressionRole of TET2VSMC apoptosisTransplant samplesGraft modelHigh-dose ascorbic acidTET2 expressionVSMC phenotypeContext of transplantCoronary blood flowEffect of IFNγTET2 activityTET2 depletionSmooth muscle cell apoptosisVascular smooth muscle cell apoptosisMuscle cell apoptosisAllograft vasculopathyDevastating sequelaeMedial thinningAortic graftHeart transplantTransplant failure
2023
The age of bone marrow dictates the clonality of smooth muscle-derived cells in atherosclerotic plaques
Kabir I, Zhang X, Dave J, Chakraborty R, Qu R, Chandran R, Ntokou A, Gallardo-Vara E, Aryal B, Rotllan N, Garcia-Milian R, Hwa J, Kluger Y, Martin K, Fernández-Hernando C, Greif D. The age of bone marrow dictates the clonality of smooth muscle-derived cells in atherosclerotic plaques. Nature Aging 2023, 3: 64-81. PMID: 36743663, PMCID: PMC9894379, DOI: 10.1038/s43587-022-00342-5.Peer-Reviewed Original ResearchMeSH KeywordsAgedAnimalsAtherosclerosisBone MarrowHumansIntegrin beta3MiceMuscle, SmoothMyocytes, Smooth MusclePlaque, AtheroscleroticConceptsAtherosclerotic plaquesBone marrowSmooth muscle-derived cellsSMC progenitorsAtherosclerotic plaque cellsSmooth muscle cell progenitorsPredominant risk factorCause of deathNovel therapeutic strategiesTNF receptor 1Muscle-derived cellsAged bone marrowAged BMEffect of agePlaque burdenAged miceRisk factorsTumor necrosisTherapeutic strategiesPlaque cellsMyeloid cellsReceptor 1Integrin β3Cell progenitorsAtherosclerosis
2019
Promoters to Study Vascular Smooth Muscle
Chakraborty R, Saddouk FZ, Carrao AC, Krause DS, Greif DM, Martin KA. Promoters to Study Vascular Smooth Muscle. Arteriosclerosis Thrombosis And Vascular Biology 2019, 39: 603-612. PMID: 30727757, PMCID: PMC6527360, DOI: 10.1161/atvbaha.119.312449.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsCell LineCell LineageCell TransdifferentiationGene Expression RegulationGene Knockout TechniquesGene TargetingHumansMiceMicrofilament ProteinsMuscle ProteinsMuscle, Smooth, VascularMyocytes, Smooth MuscleMyofibroblastsMyosin Heavy ChainsNeovascularization, PathologicNeovascularization, PhysiologicPhenotypePromoter Regions, GeneticRecombinant Fusion ProteinsConceptsSmooth muscle cellsCre driver linesDiversity of phenotypesMuscle cell typesVisceral smooth muscle cellsSMC transdifferentiationActa2 promoterRemarkable plasticityExciting new eraSMC functionCell typesCre linesEmbryonic heartExciting discoveriesPhenotypeMuscle cellsPerivascular adipocytesPromoterVascular smooth muscleNonmuscular cellsExpressionMyeloid cellsCardiovascular phenotypesCellsBlood vessel wall
2017
Chapter 8 Characterization of GPCR signaling in hypoxia
Chakraborty R, Sikarwar A, Hinton M, Dakshinamurti S, Chelikani P. Chapter 8 Characterization of GPCR signaling in hypoxia. Methods In Cell Biology 2017, 142: 101-110. PMID: 28964329, DOI: 10.1016/bs.mcb.2017.07.005.Peer-Reviewed Original ResearchConceptsThromboxane A2 receptorG protein-coupled receptorsPrimary pulmonary artery smooth muscle cellsPulmonary artery smooth muscle cellsArtery smooth muscle cellsPersistent pulmonary hypertensionPulmonary arterial responsesKey G‐protein‐coupled receptorsSmooth muscle cellsPulmonary hypertensionProtein-coupled receptorsArterial responseTissue hypoxiaCardiovascular diseaseA2 receptorsT cellsProstanoid receptorsCell responsesMuscle cellsHypoxic experimentsGPCR signalingHypoxiaHEK293T cellsReceptorsOxygen supply
2014
Dextromethorphan Mediated Bitter Taste Receptor Activation in the Pulmonary Circuit Causes Vasoconstriction
Upadhyaya J, Singh N, Sikarwar A, Chakraborty R, Pydi S, Bhullar R, Dakshinamurti S, Chelikani P. Dextromethorphan Mediated Bitter Taste Receptor Activation in the Pulmonary Circuit Causes Vasoconstriction. PLOS ONE 2014, 9: e110373. PMID: 25340739, PMCID: PMC4207743, DOI: 10.1371/journal.pone.0110373.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDextromethorphanFluorescent Antibody TechniqueGene Expression RegulationGene Knockdown TechniquesHumansIn Vitro TechniquesInositol 1,4,5-TrisphosphateLungMyocytes, Smooth MuscleMyographyMyosin Light ChainsPhosphorylationPulmonary ArteryReceptors, G-Protein-CoupledReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSuperoxidesSus scrofaTasteVasoconstrictionConceptsPulmonary artery smooth muscle cellsArtery smooth muscle cellsHuman pulmonary artery smooth muscle cellsSmooth muscle cellsAirway ringsMuscle cellsVascular tonePulmonary circuitCalcium responseHuman airway smooth muscle cellsAirway smooth muscle cellsExpression of T2RsPulmonary vascular toneBitter taste receptor activationTaste receptor activationEx vivo studyVasoconstrictor responsesBitter taste receptorsMyographic studiesMuscle relaxationReceptor activationDextromethorphanSpecific shRNART-PCRVivo studies