2014
Differential expression of bitter taste receptors in non-cancerous breast epithelial and breast cancer cells
Singh N, Chakraborty R, Bhullar R, Chelikani P. Differential expression of bitter taste receptors in non-cancerous breast epithelial and breast cancer cells. Biochemical And Biophysical Research Communications 2014, 446: 499-503. PMID: 24613843, DOI: 10.1016/j.bbrc.2014.02.140.Peer-Reviewed Original ResearchConceptsBreast cancer cellsCancer cellsBitter taste receptorsMammary epithelial cellsMetastatic breast cancer cell line MDA-MB-231Epithelial cellsBreast cancer cell line MDA-MB-231Intracellular calcium mobilizationCancer cell line MDA-MB-231Cell line MDA-MB-231Taste receptorsBitter melon extractNon-cancerous breastCell line MCF-7G protein-coupled receptorsMDA-MB-231Protein-coupled receptorsHuman bitter taste receptorsEstrogen receptorCalcium mobilizationTumor growthMelon extractFlow cytometryMCF-10A.Receptors
2012
Role of rhodopsin N-terminus in structure and function of rhodopsin-bitter taste receptor chimeras
Pydi S, Chakraborty R, Bhullar R, Chelikani P. Role of rhodopsin N-terminus in structure and function of rhodopsin-bitter taste receptor chimeras. Biochemical And Biophysical Research Communications 2012, 430: 179-182. PMID: 23159609, DOI: 10.1016/j.bbrc.2012.11.029.Peer-Reviewed Original ResearchConceptsG protein-coupled receptorsBitter taste receptorsTaste receptorsProtein-coupled receptorsBitter taste sensationReceptor expressionLow-level expressionCalcium imagingReceptor functionFlow cytometryReceptorsTaste sensationAllosteric ligand bindingT2RsReceptor chimerasAmino acidsExpressionReceptor structureN-terminal 33N-terminal 33 amino acidsN-terminal sequenceCytometry