2021
Exercise Counterbalances Rho/ROCK2 Signaling Impairment in the Skeletal Muscle and Ameliorates Insulin Sensitivity in Obese Mice
Muñoz V, Gaspar R, Severino M, Macêdo A, Simabuco F, Ropelle E, Cintra D, da Silva A, Kim Y, Pauli J. Exercise Counterbalances Rho/ROCK2 Signaling Impairment in the Skeletal Muscle and Ameliorates Insulin Sensitivity in Obese Mice. Frontiers In Immunology 2021, 12: 702025. PMID: 34234788, PMCID: PMC8256841, DOI: 10.3389/fimmu.2021.702025.Peer-Reviewed Original ResearchConceptsSkeletal muscleInsulin receptor substrate-1Protein tyrosine phosphatase 1BSkeletal muscle glucose uptakeGlucose uptakeProtein kinase BReceptor substrate-1Muscle glucose uptakePhosphatase 1BRho kinase isoformsSubstrate-1Kinase BMolecular mechanismsSystemic glucose homeostasisTensin homologC2C12 myotubesAkt phosphorylationHigher phosphorylationMolecular analysisMuscle insulinInhibitory regulatorPotential targetRhoA-ROCK2PhosphorylationROCK2 protein
2020
Effects of short-term physical training on the interleukin-15 signalling pathway and glucose tolerance in aged rats
Minuzzi L, da Conceição L, Muñoz V, Vieira R, Gaspar R, da Silva A, Cintra D, Pereira de Moura L, Ropelle E, Teixeira A, Pauli J. Effects of short-term physical training on the interleukin-15 signalling pathway and glucose tolerance in aged rats. Cytokine 2020, 137: 155306. PMID: 33010727, DOI: 10.1016/j.cyto.2020.155306.Peer-Reviewed Original ResearchConceptsAerobic physical trainingInsulin sensitivityIL-15Physical trainingGlucose toleranceInterleukin-15Elderly ratsWhole-body insulin sensitivityShort-term physical trainingSkeletal muscleLast exercise sessionExercise training protocolMesenteric fat massPhysical exercise trainingPhysical exercise protocolSkeletal muscle alterationsFourteen Wistar ratsExpression of HIF1αExercise trainingExercise protocolExercise sessionsMuscle alterationsFat massWistar ratsSupraphysiological levelsPhysical exercise increases ROCK activity in the skeletal muscle of middle-aged rats
Muñoz V, Gaspar R, Esteca M, Baptista I, Vieira R, da Silva A, de Moura L, Cintra D, Ropelle E, Pauli J. Physical exercise increases ROCK activity in the skeletal muscle of middle-aged rats. Mechanisms Of Ageing And Development 2020, 186: 111213. PMID: 32032622, DOI: 10.1016/j.mad.2020.111213.Peer-Reviewed Original ResearchConceptsMiddle-aged ratsSoleus musclePhysical exerciseGlucose homeostasisAge-related metabolic disordersSkeletal muscle glucose uptakeSkeletal muscleImpaired glucose homeostasisType 2 diabetesMuscle glucose uptakeInsulin receptor substrate-1 phosphorylationWhole-body glucose homeostasisROCK activitySkeletal muscle fiber typesMuscle fiber typesInsulin resistanceSedentary ratsHigh insulinDifferent skeletal muscle fiber typesMetabolic disordersMetabolism-related genesPharmacological inhibitionRatsGlucose uptakeMuscle
2019
Tlr4 participates in the responses of markers of apoptosis, inflammation, and ER stress to different acute exercise intensities in mice hearts
de Vicente L, Pinto A, Muñoz V, Rovina R, da Rocha A, Gaspar R, da Silva L, Simabuco F, Frantz F, Pauli J, de Moura L, Cintra D, Ropelle E, da Silva A. Tlr4 participates in the responses of markers of apoptosis, inflammation, and ER stress to different acute exercise intensities in mice hearts. Life Sciences 2019, 240: 117107. PMID: 31785241, DOI: 10.1016/j.lfs.2019.117107.Peer-Reviewed Original ResearchConceptsAcute exercise intensityAcute physical exerciseLeft ventricleTLR4 deletionIL-17Serum levelsTNF-alphaPhysical exerciseExercise intensityER stressToll-like receptor 4Heart mRNA levelsSame exercise conditionTLR4 KO miceRole of TLR4Myocardium of miceExpression of inflammationResponse of markersEndoplasmic reticulum stressKO miceReceptor 4CK-MBImmune responseInflammationER stress genesRho-kinase activity is upregulated in the skeletal muscle of aged exercised rats
Muñoz V, Gaspar R, Minuzzi L, Dos Santos Canciglieri R, da Silva A, de Moura L, Cintra D, Ropelle E, Pauli J. Rho-kinase activity is upregulated in the skeletal muscle of aged exercised rats. Experimental Gerontology 2019, 128: 110746. PMID: 31629802, DOI: 10.1016/j.exger.2019.110746.Peer-Reviewed Original ResearchAcute physical exercise increases APPL1/PI3K signaling in the hypothalamus of lean mice
Gaspar R, Muñoz V, Kuga G, Nakandakari S, Crisol B, Lenhare L, Breda L, Botezelli J, Sant'Ana M, da Silva A, Cintra D, de Moura L, Ropelle E, Pauli J. Acute physical exercise increases APPL1/PI3K signaling in the hypothalamus of lean mice. European Journal Of Neuroscience 2019, 50: 3181-3190. PMID: 31206806, DOI: 10.1111/ejn.14490.Peer-Reviewed Original ResearchConceptsLean miceExercise sessionsPhysical exerciseAcute exercise sessionAcute physical exercisePhysical exercise sessionPI3K p110PI3K mRNAAdiponectin pathwayArcuate nucleusSwiss miceHypothalamusAdiponectinEnergy homeostasisMiceMRNA levelsPI3KFood consumptionLeucine zipper motif 1BXD miceExerciseK mRNAPresent studyPositive correlationKey proteinsShort-term high-fat diet modulates several inflammatory, ER stress, and apoptosis markers in the hippocampus of young mice
Nakandakari S, Muñoz V, Kuga G, Gaspar R, Sant'Ana M, Pavan I, da Silva L, Morelli A, Simabuco F, da Silva A, de Moura L, Ropelle E, Cintra D, Pauli J. Short-term high-fat diet modulates several inflammatory, ER stress, and apoptosis markers in the hippocampus of young mice. Brain Behavior And Immunity 2019, 79: 284-293. PMID: 30797044, DOI: 10.1016/j.bbi.2019.02.016.Peer-Reviewed Original ResearchConceptsHigh-fat dietYoung miceShort-term HFD feedingMice fed high-fat dietFed high-fat dietHippocampus of miceER stressDisease developmentShort-term consumptionAlzheimer's disease developmentPotential molecular mechanismsHOMA-IRHFD feedingAD markersMicroglial cellsAD pathogenesisRisk factorsInflammatory signalsDiet modulatesΒ-amyloidCleaved caspase3HippocampusApoptosis markersHigh expressionMice
2018
The Effects of Aging on Rho-Kinase and Insulin Signaling in Skeletal Muscle and White Adipose Tissue of Rats
Muñoz V, Gaspar R, Kuga G, Pavan I, Simabuco F, da Silva A, de Moura L, Cintra D, Ropelle E, Pauli J. The Effects of Aging on Rho-Kinase and Insulin Signaling in Skeletal Muscle and White Adipose Tissue of Rats. The Journals Of Gerontology Series A 2018, 75: 432-436. PMID: 30596894, DOI: 10.1093/gerona/gly293.Peer-Reviewed Original ResearchAcute physical exercise increases the adaptor protein APPL1 in the hypothalamus of obese mice
Gaspar R, Muñoz V, Formigari G, Kuga G, Nakandakari S, Botezelli J, da Silva A, Cintra D, de Moura L, Ropelle E, Pauli J. Acute physical exercise increases the adaptor protein APPL1 in the hypothalamus of obese mice. Cytokine 2018, 110: 87-93. PMID: 29705396, DOI: 10.1016/j.cyto.2018.04.013.Peer-Reviewed Original ResearchConceptsAcute physical exerciseObese micePhysical exerciseFood intakeArcuate proopiomelanocortin neuronsSerum adiponectin levelsProtein-3 levelsEffects of leptinCentral nervous systemRole of APPL1Proopiomelanocortin neuronsAdiponectin levelsAcute exerciseNervous systemHypothalamusPathophysiological processesAdiponectinAdaptor protein APPL1MiceProtein kinase BLeucine zipper motif 1Kinase BExerciseIntakeProtein contentImpaired insulin signaling and spatial learning in middle-aged rats: The role of PTP1B
Kuga G, Muñoz V, Gaspar R, Nakandakari S, da Silva A, Botezelli J, Leme J, Gomes R, de Moura L, Cintra D, Ropelle E, Pauli J. Impaired insulin signaling and spatial learning in middle-aged rats: The role of PTP1B. Experimental Gerontology 2018, 104: 66-71. PMID: 29421605, DOI: 10.1016/j.exger.2018.02.005.Peer-Reviewed Original ResearchConceptsMiddle-aged ratsBrain-derived neurotrophic factorΒ-amyloid contentAlzheimer's diseaseInsulin resistanceTau phosphorylationMorris water maze testMiddle-aged Wistar ratsInsulin tolerance testMain risk factorsAge-related changesNeurotrophic factorTolerance testRisk factorsMaze testImpaired insulinWistar ratsNew therapiesHippocampus regionBody weightCognitive declineRole of PTP1BSynaptic plasticityRatsSpatial learningExercise increases Rho‐kinase activity and insulin signaling in skeletal muscle
Muñoz V, Gaspar R, Kuga G, da Rocha A, Crisol B, Botezelli J, Baptista I, Mekary R, da Silva A, Cintra D, de Moura L, Ropelle E, Pauli J. Exercise increases Rho‐kinase activity and insulin signaling in skeletal muscle. Journal Of Cellular Physiology 2018, 233: 4791-4800. PMID: 29219181, DOI: 10.1002/jcp.26278.Peer-Reviewed Original Research