2016
N-glycosylation critically regulates function of oxalate transporter SLC26A6
Thomson RB, Thomson CL, Aronson PS. N-glycosylation critically regulates function of oxalate transporter SLC26A6. American Journal Of Physiology - Cell Physiology 2016, 311: c866-c873. PMID: 27681177, PMCID: PMC5206297, DOI: 10.1152/ajpcell.00171.2016.Peer-Reviewed Original ResearchConceptsPlasma membraneIntegral membrane proteinsCell surface deliverySLC26A6 functionTissue-specific differencesGlycosylation mutantsMembrane proteinsN-glycosylationSurface deliveryBiotinylation studiesOxalate transporterOxalate homeostasisSecond extracellular loopExtracellular loopIntact cellsEnzymatic deglycosylation studiesTransport activityEnzymatic deglycosylationFunctional studiesDeglycosylation studiesGlycosylationPutative second extracellular loopTransport functionFunctional significanceEssential role
2014
Cyclic GMP Kinase II (cGKII) Inhibits NHE3 by Altering Its Trafficking and Phosphorylating NHE3 at Three Required Sites IDENTIFICATION OF A MULTIFUNCTIONAL PHOSPHORYLATION SITE*
Chen T, Kocinsky HS, Cha B, Murtazina R, Yang J, Tse CM, Singh V, Cole R, Aronson PS, de Jonge H, Sarker R, Donowitz M. Cyclic GMP Kinase II (cGKII) Inhibits NHE3 by Altering Its Trafficking and Phosphorylating NHE3 at Three Required Sites IDENTIFICATION OF A MULTIFUNCTIONAL PHOSPHORYLATION SITE*. Journal Of Biological Chemistry 2014, 290: 1952-1965. PMID: 25480791, PMCID: PMC4303652, DOI: 10.1074/jbc.m114.590174.Peer-Reviewed Original ResearchAnimalsBinding SitesCaco-2 CellsCell MembraneCyclic GMP-Dependent Protein Kinase Type IIDexamethasoneHumansIntestinal MucosaMass SpectrometryMiceMicrovilliMutagenesisPhosphorylationProtein Structure, TertiaryProtein TransportSerineSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSurface PropertiesTransfection
2007
Membrane Curvature Alters the Activation Kinetics of the Epithelial Na+/H+ Exchanger, NHE3*
Alexander RT, Malevanets A, Durkan AM, Kocinsky HS, Aronson PS, Orlowski J, Grinstein S. Membrane Curvature Alters the Activation Kinetics of the Epithelial Na+/H+ Exchanger, NHE3*. Journal Of Biological Chemistry 2007, 282: 7376-7384. PMID: 17218318, DOI: 10.1074/jbc.m608557200.Peer-Reviewed Original Research
2006
Regulation of anion exchanger Slc26a6 by protein kinase C
Hassan HA, Mentone S, Karniski LP, Rajendran VM, Aronson PS. Regulation of anion exchanger Slc26a6 by protein kinase C. American Journal Of Physiology - Cell Physiology 2006, 292: c1485-c1492. PMID: 17151144, DOI: 10.1152/ajpcell.00447.2006.Peer-Reviewed Original ResearchMeSH KeywordsAcetophenonesAnimalsAnion Transport ProteinsAntiportersBenzopyransBiological Transport, ActiveCarbazolesCell MembraneChloridesCytoplasmEnzyme ActivationFemaleFormatesIn Vitro TechniquesIndolesMaleimidesMiceOocytesOxalatesProtein Kinase C-deltaProtein TransportSulfate TransportersTetradecanoylphorbol AcetateXenopusConceptsPKC activationOxalate secretionIntestinal oxalate secretionFormate exchangeRenal proximal tubulesAnion exchanger pendrinPancreatic ductExchanger pendrinProximal tubulesSmall intestineSLC26A6 expressionNaCl reabsorptionCalcium independentSimilar extentPotential rolePKC-delta activationProtein kinase CActivationSecretionSlc26a6Immunofluorescence microscopyXenopus oocytesPMAPhysiological significanceOocyte plasma membraneRole of SLC26‐Mediated Cl−/Base Exchange in Proximal Tubule NaCl Transport
Aronson PS. Role of SLC26‐Mediated Cl−/Base Exchange in Proximal Tubule NaCl Transport. Novartis Foundation Symposia 2006, 273: 148-163. PMID: 17120766, DOI: 10.1002/0470029579.ch10.Commentaries, Editorials and LettersThe life-extending gene Indy encodes an exchanger for Krebs-cycle intermediates
Knauf F, Mohebbi N, Teichert C, Herold D, Rogina B, Helfand S, Gollasch M, Luft FC, Aronson PS. The life-extending gene Indy encodes an exchanger for Krebs-cycle intermediates. Biochemical Journal 2006, 397: 25-29. PMID: 16608441, PMCID: PMC1479758, DOI: 10.1042/bj20060409.Peer-Reviewed Original ResearchConceptsExternal citrateKrebs cycle intermediatesXenopus oocytesSodium dicarboxylate cotransporterMammalian genesDrosophila melanogasterIndy mutantsLifespan extensionLongevity genesPlasma membraneSubstrate effluxExternal mediumSpecific transport mechanismsFunctional studiesSuccinate uptakeAddition of succinateEnergy metabolismIndyTransportable substratesGenesOocytesEffluxMelanogasterMutantsSuccinate
2005
Role of PDZK1 in membrane expression of renal brush border ion exchangers
Thomson RB, Wang T, Thomson BR, Tarrats L, Girardi A, Mentone S, Soleimani M, Kocher O, Aronson PS. Role of PDZK1 in membrane expression of renal brush border ion exchangers. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 13331-13336. PMID: 16141316, PMCID: PMC1201624, DOI: 10.1073/pnas.0506578102.Peer-Reviewed Original ResearchConceptsProximal tubulesExpression of NHE3Functional activityRole of PDZK1Protein PDZK1Reabsorption of NaBrush border expressionMammalian kidneyCFEXBrush border localizationMutant miceGST fusion proteinProtein expressionKidneyBrush border membrane proteinsExchanger NHE3Membrane expressionNHE3Brush borderBrush border membrane vesiclesPDZK1 interactionTubulesNormal expressionMembrane proteinsPDZK1
2004
The Cl−/HCO3− exchanger pendrin in the rat kidney is regulated in response to chronic alterations in chloride balance
Quentin F, Chambrey R, Trinh-Trang-Tan MM, Fysekidis M, Cambillau M, Paillard M, Aronson PS, Eladari D. The Cl−/HCO3− exchanger pendrin in the rat kidney is regulated in response to chronic alterations in chloride balance. American Journal Of Physiology. Renal Physiology 2004, 287: f1179-f1188. PMID: 15292050, DOI: 10.1152/ajprenal.00211.2004.Peer-Reviewed Original ResearchConceptsChronic furosemide administrationBlood pressure regulationDiet-induced changesAcid-base statusNaCl dietFurosemide administrationChronic alterationsControl ratsRenal membrane fractionsChloride balanceChronic changesNaCl balanceExchanger pendrinSemiquantitative immunoblottingPressure regulationRat kidneyConsistent inverse relationshipPD expressionNaCl depletionType BProtein abundanceRatsPendrinInverse relationshipExpression
2002
Functional characterization and immunolocalization of the transporter encoded by the life-extending gene Indy
Knauf F, Rogina B, Jiang Z, Aronson PS, Helfand SL. Functional characterization and immunolocalization of the transporter encoded by the life-extending gene Indy. Proceedings Of The National Academy Of Sciences Of The United States Of America 2002, 99: 14315-14319. PMID: 12391301, PMCID: PMC137881, DOI: 10.1073/pnas.222531899.Peer-Reviewed Original ResearchConceptsPlasma membraneLife-extending mutationsNew longevity genesIntermediary metabolismAdult fat bodyIndy expressionCitric acid cycleLife-extending effectsMetabolite transportersVariety of speciesLongevity genesFunctional characterizationLife spanMidgut cellsFat bodyAcid cycleFunctional studiesXenopus oocytesTransport functionIndyBasolateral regionSodium-independent mechanismProminent expressionAverage life spanTransporters
2001
Role of sodium/hydrogen exchanger isoform NHE3 in fluid secretion and absorption in mouse and rat cholangiocytes
Mennone A, Biemesderfer D, Negoianu D, Yang C, Abbiati T, Schultheis P, Shull G, Aronson P, Boyer J. Role of sodium/hydrogen exchanger isoform NHE3 in fluid secretion and absorption in mouse and rat cholangiocytes. AJP Gastrointestinal And Liver Physiology 2001, 280: g247-g254. PMID: 11208547, DOI: 10.1152/ajpgi.2001.280.2.g247.Peer-Reviewed Original Research
2000
Active (9.6 S) and Inactive (21 S) Oligomers of NHE3 in Microdomains of the Renal Brush Border*
Biemesderfer D, DeGray B, Aronson P. Active (9.6 S) and Inactive (21 S) Oligomers of NHE3 in Microdomains of the Renal Brush Border*. Journal Of Biological Chemistry 2000, 276: 10161-10167. PMID: 11120742, DOI: 10.1074/jbc.m008098200.Peer-Reviewed Original ResearchAcridine OrangeAnimalsAntibodiesCarrier ProteinsCell MembraneCentrifugation, Density GradientElectrophoresis, Polyacrylamide GelEndosomesFluorescent Antibody Technique, IndirectGoatsHeymann Nephritis Antigenic ComplexHydrogenHydrogen-Ion ConcentrationImmunoblottingImmunohistochemistryKidneyKidney TubulesMagnesiumMaleMembrane GlycoproteinsMicrofilament ProteinsMicroscopy, ElectronMicrovilliMitochondriaPrecipitin TestsProtein BindingProtein Structure, TertiaryRabbitsRatsRats, Sprague-DawleySodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSucroseTime Factors
1997
Expression of Na(+)-H+ exchanger isoforms NHE1 and NHE3 in kidney and blood cells of rabbit and rat.
Rutherford PA, Pizzonia JH, Biemesderfer D, Abu-Alfa A, Reilly R, Aronson PS. Expression of Na(+)-H+ exchanger isoforms NHE1 and NHE3 in kidney and blood cells of rabbit and rat. Nephron 1997, 5: 490-7. PMID: 9438178.Peer-Reviewed Original ResearchConceptsNa-H exchangeNa-H exchangerBlood cellsRat kidneyNa-Li countertransportNHE1 protein expressionExpression of NHE1Diabetic nephropathyCountertransport activityIsoform-specific antibodiesMonoclonal antibodiesExchanger isoforms NHE1NHE3 expressionRenal brush border membraneProtein expressionBrush border membraneKidneyNHE isoformsRabbitsNHE3Blood cell membranesBrush border membrane fractionRatsIsoforms NHE1Antibodies
1992
Inhibition of Ca-activated K+ channels from renal microvillus membrane vesicles by amiloride analogs
Zweifach A, Desir G, Aronson P, Giebisch G. Inhibition of Ca-activated K+ channels from renal microvillus membrane vesicles by amiloride analogs. The Journal Of Membrane Biology 1992, 128: 115-122. PMID: 1501239, DOI: 10.1007/bf00231884.Peer-Reviewed Original Research
1991
Molecular biology of renal Na(+)-H+ exchangers.
Igarashi P, Reilly RF, Hildebrandt F, Biemesderfer D, Rebouças NA, Slayman CW, Aronson PS. Molecular biology of renal Na(+)-H+ exchangers. Kidney International Supplement 1991, 33: s84-9. PMID: 1653876.Commentaries, Editorials and LettersConceptsKb cDNALLC-PK1 cellsSteady-state transcript levelsPlasma membrane proteinsMembrane-associated domainSequence of cDNACDNA library screeningAmino acid sequenceExchanger gene expressionNorthern blot analysisEvolutionary conservationRenal epithelial cellsCytoplasmic domainMembrane proteinsRegulation of intracellularAcid sequenceTranscript levelsUntranslated regionGene expressionSimilar transcriptsCDNALibrary screeningMolecular biologyPolymerase chain reactionBlot analysisCation specificity and modes of the Na+:CO3(2-):HCO3- cotransporter in renal basolateral membrane vesicles
Soleimani M, Lesoine GA, Bergman JA, Aronson PS. Cation specificity and modes of the Na+:CO3(2-):HCO3- cotransporter in renal basolateral membrane vesicles. Journal Of Biological Chemistry 1991, 266: 8706-8710. PMID: 2026588, DOI: 10.1016/s0021-9258(18)31503-5.Peer-Reviewed Original ResearchIncreased Na(+)-H+ antiporter activity in apical membrane vesicles from mutant LLC-PK1 cells
Reilly RF, Haggerty JG, Aronson PS, Adelberg EA, Slayman CW. Increased Na(+)-H+ antiporter activity in apical membrane vesicles from mutant LLC-PK1 cells. American Journal Of Physiology 1991, 260: c738-c744. PMID: 1673319, DOI: 10.1152/ajpcell.1991.260.4.c738.Peer-Reviewed Original ResearchHigh affinity binding of amiloride analogs at an internal site in renal microvillus membrane vesicles
Desir GV, Cragoe EJ, Aronson PS. High affinity binding of amiloride analogs at an internal site in renal microvillus membrane vesicles. Journal Of Biological Chemistry 1991, 266: 2267-2271. PMID: 1846621, DOI: 10.1016/s0021-9258(18)52238-9.Peer-Reviewed Original ResearchConceptsMembrane vesiclesMicrovillus membrane vesiclesRenal microvillus membrane vesiclesSimilar rank order potencyBinding sitesInternal sitesHigh-affinity inhibitorsMembrane localizationInternal binding siteAmiloride analoguesPlasma membraneRank order potencyHigh-affinity bindingAffinity inhibitorsHigh affinity-binding siteVesiclesBasolateral membrane vesiclesOrder potency
1990
Effects of apical membrane Cl(-)-formate exchange on cell volume in rabbit proximal tubule
Schild L, Aronson PS, Giebisch G. Effects of apical membrane Cl(-)-formate exchange on cell volume in rabbit proximal tubule. American Journal Of Physiology 1990, 258: f530-f536. PMID: 2316663, DOI: 10.1152/ajprenal.1990.258.3.f530.Peer-Reviewed Original Research
1989
Ionic mechanism of Na+-HCO3 − cotransport in rabbit renal basolateral membrane vesicles*
Soleimani M, Aronson PS. Ionic mechanism of Na+-HCO3 − cotransport in rabbit renal basolateral membrane vesicles*. Journal Of Biological Chemistry 1989, 264: 18302-18308. PMID: 2509453, DOI: 10.1016/s0021-9258(18)51463-0.Peer-Reviewed Original Research4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAnimalsAnionsBicarbonatesBinding, CompetitiveBiological TransportCarbon DioxideCarrier ProteinsCell MembraneHydrogen-Ion ConcentrationKidneyLithiumMaleRabbitsSodiumSodium-Bicarbonate SymportersStilbenesSulfitesEffects of acetazolamide on Na+-HCO-3 cotransport in basolateral membrane vesicles isolated from rabbit renal cortex.
Soleimani M, Aronson PS. Effects of acetazolamide on Na+-HCO-3 cotransport in basolateral membrane vesicles isolated from rabbit renal cortex. Journal Of Clinical Investigation 1989, 83: 945-951. PMID: 2921327, PMCID: PMC303770, DOI: 10.1172/jci113980.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAcetatesAcetazolamideAmmoniaAnimalsBicarbonatesBiological TransportCarrier ProteinsCell MembraneHydrogen-Ion ConcentrationKidney CortexMaleRabbitsSodiumSodium-Bicarbonate Symporters