2016
N-glycosylation critically regulates function of oxalate transporter SLC26A6
Thomson RB, Thomson CL, Aronson PS. N-glycosylation critically regulates function of oxalate transporter SLC26A6. American Journal Of Physiology - Cell Physiology 2016, 311: c866-c873. PMID: 27681177, PMCID: PMC5206297, DOI: 10.1152/ajpcell.00171.2016.Peer-Reviewed Original ResearchConceptsPlasma membraneIntegral membrane proteinsCell surface deliverySLC26A6 functionTissue-specific differencesGlycosylation mutantsMembrane proteinsN-glycosylationSurface deliveryBiotinylation studiesOxalate transporterOxalate homeostasisSecond extracellular loopExtracellular loopIntact cellsEnzymatic deglycosylation studiesTransport activityEnzymatic deglycosylationFunctional studiesDeglycosylation studiesGlycosylationPutative second extracellular loopTransport functionFunctional significanceEssential role
2013
Ezrin Is Required for the Functional Regulation of the Epithelial Sodium Proton Exchanger, NHE3
Hayashi H, Tamura A, Krishnan D, Tsukita S, Suzuki Y, Kocinsky HS, Aronson PS, Orlowski J, Grinstein S, Alexander RT. Ezrin Is Required for the Functional Regulation of the Epithelial Sodium Proton Exchanger, NHE3. PLOS ONE 2013, 8: e55623. PMID: 23405179, PMCID: PMC3566197, DOI: 10.1371/journal.pone.0055623.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonAnimalsColonCyclic AMPCytoskeletal ProteinsDogsEpithelial CellsFluorescence Recovery After PhotobleachingHumansMadin Darby Canine Kidney CellsMaleMembrane ProteinsMiceMice, KnockoutMicrofilament ProteinsMicrovilliOctoxynolPhosphorylationProtein TransportRNA, Small InterferingSodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersConceptsApical actin cytoskeletonNHE3 activitySodium hydrogen exchanger isoform 3CAMP-dependent inhibitionEzrin knockdown miceERM proteinsActin cytoskeletonSodium-proton exchangerApical cytoskeletonApical localizationFunctional regulationWild-type animalsEpithelial cell culture modelEzrinProton exchangersFluorescent recoveryEpithelial phenotypeCytoskeletonMolecular determinantsCell culture modelExchanger isoform 3Functional studiesNon-targeting siRNAApical membraneIsoform 3
2006
The life-extending gene Indy encodes an exchanger for Krebs-cycle intermediates
Knauf F, Mohebbi N, Teichert C, Herold D, Rogina B, Helfand S, Gollasch M, Luft FC, Aronson PS. The life-extending gene Indy encodes an exchanger for Krebs-cycle intermediates. Biochemical Journal 2006, 397: 25-29. PMID: 16608441, PMCID: PMC1479758, DOI: 10.1042/bj20060409.Peer-Reviewed Original ResearchConceptsExternal citrateKrebs cycle intermediatesXenopus oocytesSodium dicarboxylate cotransporterMammalian genesDrosophila melanogasterIndy mutantsLifespan extensionLongevity genesPlasma membraneSubstrate effluxExternal mediumSpecific transport mechanismsFunctional studiesSuccinate uptakeAddition of succinateEnergy metabolismIndyTransportable substratesGenesOocytesEffluxMelanogasterMutantsSuccinate
2002
Functional characterization and immunolocalization of the transporter encoded by the life-extending gene Indy
Knauf F, Rogina B, Jiang Z, Aronson PS, Helfand SL. Functional characterization and immunolocalization of the transporter encoded by the life-extending gene Indy. Proceedings Of The National Academy Of Sciences Of The United States Of America 2002, 99: 14315-14319. PMID: 12391301, PMCID: PMC137881, DOI: 10.1073/pnas.222531899.Peer-Reviewed Original ResearchConceptsPlasma membraneLife-extending mutationsNew longevity genesIntermediary metabolismAdult fat bodyIndy expressionCitric acid cycleLife-extending effectsMetabolite transportersVariety of speciesLongevity genesFunctional characterizationLife spanMidgut cellsFat bodyAcid cycleFunctional studiesXenopus oocytesTransport functionIndyBasolateral regionSodium-independent mechanismProminent expressionAverage life spanTransporters