2021
Data-Dependent Acquisition Ladder for Capillary Electrophoresis Mass Spectrometry-Based Ultrasensitive (Neuro)Proteomics
Choi S, Muñoz-LLancao P, Manzini M, Nemes P. Data-Dependent Acquisition Ladder for Capillary Electrophoresis Mass Spectrometry-Based Ultrasensitive (Neuro)Proteomics. Analytical Chemistry 2021, 93: 15964-15972. PMID: 34812615, DOI: 10.1021/acs.analchem.1c03327.Peer-Reviewed Original ResearchConceptsData-dependent acquisitionHigh-resolution mass spectrometryTraditional data-dependent acquisitionAnalytical toolbox of neuroscienceCE-ESIElectrospray ionizationUltrasensitive proteomicsDynamic exclusionMass spectrometryPeptide signalsReplicate measurementsTrace amountsNeuronal marker genesIdentification success rateAnalytical toolboxIdentified proteinsNonredundant proteinsData acquisition strategyMarker genesProtein digestibilityProteinNeurobiological importanceMolecular pathwaysCultured hippocampalIonization
2017
Upregulation of Epac-1 in hepatic stellate cells by Prostaglandin E2 in liver fibrosis is associated with reduced fibrogenesis.
Schippers M, Beljaars L, Post E, Lotersztajn S, Reker-Smit C, Han B, Munoz-Llancao P, Schmidt M, Poelstra K. Upregulation of Epac-1 in hepatic stellate cells by Prostaglandin E2 in liver fibrosis is associated with reduced fibrogenesis. Journal Of Pharmacology And Experimental Therapeutics 2017, 363: jpet.117.241646. PMID: 28864467, DOI: 10.1124/jpet.117.241646.Peer-Reviewed Original ResearchConceptsEpac-1 activationEpac-1Hepatic stellate cellsNiflumic acidPlatelet-derived growth factor-induced proliferationLiver fibrosisFibrotic livers of miceReduced Rho-kinase activityKupffer cellsIsolated hepatic stellate cellsStellate cellsCAMP-mediated effectsGrowth factor-induced proliferationWeeks carbon tetrachlorideEffect of PGE<sub>2</sub> onEPAC-1 expressionKinase activityProtein kinase A expressionRho-kinase activityWestern blot analysisActivity in vitroProstaglandin E<sub>2</sub>Cyclo-oxygenase productsDownstream targetsCell-specific delivery
2015
Exchange Protein Directly Activated by cAMP (EPAC) Regulates Neuronal Polarization through Rap1B
Muñoz-Llancao P, Henríquez D, Wilson C, Bodaleo F, Boddeke E, Lezoualc'h F, Schmidt M, González-Billault C. Exchange Protein Directly Activated by cAMP (EPAC) Regulates Neuronal Polarization through Rap1B. Journal Of Neuroscience 2015, 35: 11315-11329. PMID: 26269639, PMCID: PMC6605123, DOI: 10.1523/jneurosci.3645-14.2015.Peer-Reviewed Original ResearchConceptsNeuronal polarityAcquisition of neuronal polarityAxon specificationGenetic manipulationRap1b activationExchange proteinGuanine exchange factorKnock-outEpac signalingRegulate neuronal polarityProtein kinase A.Axon determinationRap GTPasesWild-type neuronsExchange factorActivation of protein kinase A.Polarity defectsMouse neuronsRat hippocampal neuronsMessenger cAMPMolecular eventsEpacMature axonsCAMP-dependent mechanismPharmacological inhibition