2023
A kinesin-1 adaptor complex controls bimodal slow axonal transport of spectrin in Caenorhabditis elegans
Glomb O, Swaim G, Munoz LLancao P, Lovejoy C, Sutradhar S, Park J, Wu Y, Cason S, Holzbaur E, Hammarlund M, Howard J, Ferguson S, Gramlich M, Yogev S. A kinesin-1 adaptor complex controls bimodal slow axonal transport of spectrin in Caenorhabditis elegans. Developmental Cell 2023, 58: 1847-1863.e12. PMID: 37751746, PMCID: PMC10574138, DOI: 10.1016/j.devcel.2023.08.031.Peer-Reviewed Original Research
2022
Capillary Electrophoresis Mass Spectrometry for Scalable Single-Cell Proteomics
Shen B, Pade L, Choi S, Muñoz-LLancao P, Manzini M, Nemes P. Capillary Electrophoresis Mass Spectrometry for Scalable Single-Cell Proteomics. Frontiers In Chemistry 2022, 10: 863979. PMID: 35464213, PMCID: PMC9024316, DOI: 10.3389/fchem.2022.863979.Peer-Reviewed Original ResearchHigh-resolution mass spectrometryCapillary electrophoresisElectrospray ionization (ESISingle-cell proteomicsMass spectrometryCapillary electrophoresis mass spectrometryNano-flow liquid chromatographySample preparationSingle-cell technologiesSample amountCapillary microsamplingSubcellular proteomicsCellular dimensionsLiquid chromatographyMidline cellsSpectrometryProteomicsSubcellular analysisProtein groupsProteinMouse hippocampusSerial dilutionProtein digestibilityESINeurons
2021
Data-Dependent Acquisition Ladder for Capillary Electrophoresis Mass Spectrometry-Based Ultrasensitive (Neuro)Proteomics
Choi S, Muñoz-LLancao P, Manzini M, Nemes P. Data-Dependent Acquisition Ladder for Capillary Electrophoresis Mass Spectrometry-Based Ultrasensitive (Neuro)Proteomics. Analytical Chemistry 2021, 93: 15964-15972. PMID: 34812615, DOI: 10.1021/acs.analchem.1c03327.Peer-Reviewed Original ResearchConceptsData-dependent acquisitionHigh-resolution mass spectrometryTraditional data-dependent acquisitionAnalytical toolbox of neuroscienceCE-ESIElectrospray ionizationUltrasensitive proteomicsDynamic exclusionMass spectrometryPeptide signalsReplicate measurementsTrace amountsNeuronal marker genesIdentification success rateAnalytical toolboxIdentified proteinsNonredundant proteinsData acquisition strategyMarker genesProtein digestibilityProteinNeurobiological importanceMolecular pathwaysCultured hippocampalIonization
2014
A‐kinase anchoring proteins: cAMP compartmentalization in neurodegenerative and obstructive pulmonary diseases
Poppinga W, Muñoz‐Llancao P, González‐Billault C, Schmidt M. A‐kinase anchoring proteins: cAMP compartmentalization in neurodegenerative and obstructive pulmonary diseases. British Journal Of Pharmacology 2014, 171: 5603-5623. PMID: 25132049, PMCID: PMC4290705, DOI: 10.1111/bph.12882.Peer-Reviewed Original ResearchConceptsA-kinase anchoring proteinsCAMP compartmentationCAMP effector proteinsCompartmentalized cAMP signalingGs protein-coupled receptorsCellular content of cAMPEffector proteinsCompartmentalization of cAMPAnchoring proteinsCAMP compartmentCellular functionsContent of cAMPA-kinaseCAMP signalingProtein-coupled receptorsActivation of PKAObstructive pulmonary diseaseMessenger cAMPExchange proteinCellular contentAlzheimer's diseaseNeurodegenerative diseasesProteinB2-adrenoceptorsPulmonary disease