1996
Cis preference of the IS 903 transposase is mediated by a combination of transposase instability and inefficient translation
Derbyshire K, Grindley N. Cis preference of the IS 903 transposase is mediated by a combination of transposase instability and inefficient translation. Molecular Microbiology 1996, 21: 1261-1272. PMID: 8898394, DOI: 10.1111/j.1365-2958.1996.tb02587.x.Peer-Reviewed Original ResearchConceptsClasses of mutationsLevel of transpositionDNA-binding proteinsCis-acting proteinsAmount of transposaseCis preferenceWild-type transposaseInefficient translation initiationSite of synthesisAmino acids 25Translation initiationTranslational initiationTransposase proteinTranslation efficiencyMutant geneGene expressionProtein instabilityTransposase geneInefficient translationProline substitutionTransposaseMutant transposaseMutationsProteinUnusual class
1990
Role of instability in the cis action of the insertion sequence IS903 transposase.
Derbyshire K, Kramer M, Grindley N. Role of instability in the cis action of the insertion sequence IS903 transposase. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 4048-4052. PMID: 2161528, PMCID: PMC54044, DOI: 10.1073/pnas.87.11.4048.Peer-Reviewed Original ResearchConceptsDNA-binding proteinsCis actionCis-acting proteinsKilobase of DNAInsertion sequenceGalactosidase fusion proteinWild-type transposaseProtease-deficient strainSite of synthesisLon geneBacterial insertion sequencesUnstable proteinTransposase geneFusion proteinTrans actionTransposaseEscherichia coliProteinDefective transposonTransposonGenes