2021
Diagnostic Accuracy of Serum Hyaluronan for Detecting HCV Infection and Liver Fibrosis in Asymptomatic Blood Donors
Rodart I, Pares M, Mendes A, Accardo C, Martins J, Silva C, Carvalho F, Barreto J, Reis M, Tersariol I, Nader H. Diagnostic Accuracy of Serum Hyaluronan for Detecting HCV Infection and Liver Fibrosis in Asymptomatic Blood Donors. Molecules 2021, 26: 3892. PMID: 34202190, PMCID: PMC8270308, DOI: 10.3390/molecules26133892.Peer-Reviewed Original ResearchMeSH KeywordsAdultBlood DonorsEnzyme-Linked Immunosorbent AssayFemaleGenotypeHepacivirusHepatitis CHumansHyaluronic AcidLiver CirrhosisMaleMiddle AgedConceptsHepatitis C virusAsymptomatic blood donorsLiver fibrosisBlood donorsSerum hyaluronanHCV infectionSerum levelsGGT serum levelsHA serum levelsHCV RNA detectionHCV viral loadProgressive liver diseaseAdvanced liver fibrosisHigher ALT levelsSignificant liver fibrosisNormal ALTHCV viremiaALT levelsAntiviral treatmentAsymptomatic personsBiopsy confirmationHCV samplesLiver biomarkersLiver diseaseViral loadRisk of chronic arthralgia and impact of pain on daily activities in a cohort of patients with chikungunya virus infection from Brazil
Silva M, Kikuti M, Anjos R, Portilho M, Santos V, Gonçalves T, Tauro L, Moreira P, Jacob-Nascimento L, Santana P, Campos G, Siqueira A, Kitron U, Reis M, Ribeiro G. Risk of chronic arthralgia and impact of pain on daily activities in a cohort of patients with chikungunya virus infection from Brazil. International Journal Of Infectious Diseases 2021, 105: 608-616. PMID: 33684559, DOI: 10.1016/j.ijid.2021.03.003.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultArthralgiaBrazilChikungunya FeverChikungunya virusChildChild, PreschoolChronic PainCohort StudiesEnzyme-Linked Immunosorbent AssayFemaleHumansInfantMaleMiddle AgedOdds RatioRisk FactorsYoung AdultConceptsChronic arthralgiaChikungunya virus infectionRisk factorsVirus infectionDaily activitiesAcute febrile illnessIndependent risk factorPersistence of painCohort of patientsImpact of painProgression of diseaseTranscriptase-polymerase chain reactionPersistent arthralgiaFebrile illnessSubstantial disabilityViral loadFemale sexPolymerase chain reactionIgM enzymeArthralgiaSurveillance studyChikungunya virusDengue virusPatientsImmunosorbent assay
2019
Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses
Kikuti M, Tauro L, Moreira P, Nascimento L, Portilho M, Soares G, Weaver S, Reis M, Kitron U, Ribeiro G. Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses. International Journal Of Infectious Diseases 2019, 91: 38-43. PMID: 31704226, DOI: 10.1016/j.ijid.2019.11.001.Peer-Reviewed Original ResearchConceptsConvalescent samplesIgM enzymeRT-PCRZika virus transmissionNegative patientsCHIKV infectionAcute samplesZIKV casesConvalescent seraSurveillance studyCHIKV transmissionZika virusCHIKV casesDiagnostic performanceLow specificityVirus transmissionEquivocal resultsTest sensitivitySerumELISA specificityPatientsHigh rateDengueConcomitant transmissionMore equivocal resultsA localized outbreak of Chikungunya virus in Salvador, Bahia, Brazil
Tauro L, Cardoso C, Souza R, Nascimento L, dos Santos D, Campos G, Sardi S, dos Reis O, Reis M, Kitron U, Ribeiro G. A localized outbreak of Chikungunya virus in Salvador, Bahia, Brazil. Memórias Do Instituto Oswaldo Cruz 2019, 114: e180597. PMID: 30843962, PMCID: PMC6396974, DOI: 10.1590/0074-02760180597.Peer-Reviewed Original ResearchMeSH KeywordsAdultBrazilChikungunya FeverChikungunya virusDisease OutbreaksEnzyme-Linked Immunosorbent AssayFemaleGenotypeHumansMaleMiddle AgedPhylogenyPolymerase Chain ReactionSeasonsYoung Adult
2014
Anti-PR3 and anti-MPO antibodies are not present in sera of patients with pulmonary tuberculosis
Lima I, Oliveira R, Cabral M, Atta A, Marchi S, Reis E, Reis M, Barbosa L, Santiago M. Anti-PR3 and anti-MPO antibodies are not present in sera of patients with pulmonary tuberculosis. Rheumatology International 2014, 34: 1231-1234. PMID: 24719228, DOI: 10.1007/s00296-014-3009-z.Peer-Reviewed Original ResearchConceptsAnti-neutrophil cytoplasmic antibodiesAnti-PR3 antibodiesIndirect immunofluorescencePresence of ANCAPrevalence of ANCAInfectious diseasesComponents of neutrophilsGroup of patientsAnti-MPO antibodiesSera of patientsChest X-rayAnti-PR3Cytoplasmic antibodiesVasculitic syndromesTB patientsPulmonary tuberculosisAutoimmune disordersPresent studyClinical similaritiesSuch antibodiesPatientsELISA kitTuberculosisAntibodiesAutoantibodies
2013
Antibodies to citrullinated peptides in tuberculosis
Lima I, Oliveira R, Atta A, Marchi S, Barbosa L, Reis E, Reis M, Santiago M. Antibodies to citrullinated peptides in tuberculosis. Clinical Rheumatology 2013, 32: 685-687. PMID: 23344687, DOI: 10.1007/s10067-013-2173-y.Peer-Reviewed Original ResearchConceptsAnti-citrullinated peptide antibodiesAnti-MCV antibodiesRheumatoid arthritisHealthy controlsRheumatoid factor positivityRA patientsSymmetric polyarthritisTB patientsPulmonary tuberculosisBone erosionCCP antibodyAutoimmune diseasesHigh incidencePeptide antibodiesPatientsControl groupHigh specificityTuberculosisInfectious diseasesAntibodiesPositivityDiseaseSpecificityMethodological factorsPolyarthritis
2011
TNF-alpha and IL-8: Serum levels and gene polymorphisms (−308G>A and −251A>T) are associated with classical biomarkers and medical history in children with sickle cell anemia
Cajado C, Cerqueira B, Couto F, Moura-Neto J, Vilas-Boas W, Dorea M, Lyra I, Barbosa C, Reis M, Goncalves M. TNF-alpha and IL-8: Serum levels and gene polymorphisms (−308G>A and −251A>T) are associated with classical biomarkers and medical history in children with sickle cell anemia. Cytokine 2011, 56: 312-317. PMID: 21802960, DOI: 10.1016/j.cyto.2011.07.002.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAllelesAnemia, Sickle CellBiomarkersCase-Control StudiesChildChild, PreschoolCross-Sectional StudiesEnzyme-Linked Immunosorbent AssayHaplotypesHumansInterleukin-8Medical History TakingPolymerase Chain ReactionPolymorphism, GeneticPolymorphism, Restriction Fragment LengthTumor Necrosis Factor-alphaConceptsSickle cell anemiaTNF-alpha -308GT gene polymorphismGene polymorphismsIL-8Serum levelsMedical historyTNF-alphaCell anemiaHigher TNF-alpha levelsIL-8 gene polymorphismsA alleleClassical biomarkersSteady-state sickle cell anemiaTNF-alpha levelsHeterogeneous clinical outcomesLogistic regression analysisYears of ageCytokine levelsClinical outcomesImmunological biomarkersHealthy controlsPatient outcomesInterleukin-8SCA patients
2008
Schistosoma mansoni Triose Phosphate Isomerase Peptide MAP4 is Able to Trigger Naïve Donor Immune Response Towards a Type‐1 Cytokine Profile
Reis E, Carmo T, Athanazio R, Reis M, Harn D. Schistosoma mansoni Triose Phosphate Isomerase Peptide MAP4 is Able to Trigger Naïve Donor Immune Response Towards a Type‐1 Cytokine Profile. Scandinavian Journal Of Immunology 2008, 68: 169-176. PMID: 18565118, DOI: 10.1111/j.1365-3083.2008.02131.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, HelminthCytokinesDendritic CellsEnzyme-Linked Immunosorbent AssayFlow CytometryHumansLymphocyte ActivationPeptidesSchistosoma mansoniTh1 CellsT-Lymphocyte SubsetsTriose-Phosphate IsomeraseConceptsPeripheral blood mononuclear cellsSchistosoma mansoni soluble egg antigenDendritic cellsCytokine productionAutologous peripheral blood mononuclear cellsType 1 cytokine profileMonocyte-derived dendritic cellsDonor dendritic cellsDonor immune responsesTh1-type responseT cell subsetsBlood mononuclear cellsIFN-gamma productionT cell epitopesMultiple antigen peptideMajor cell populationRecall stimulationCytokine profileIL-10Egg antigenMononuclear cellsT cellsImmune responseIFN-gammaMouse model
2006
Prevalence of hepatitis C virus (HCV) infection and HCV genotypes of hemodialysis patients in Salvador, Northeastern Brazil
Silva L, Silva M, Rodart I, Lopes G, Costa F, Melo M, Gusmão E, Reis M. Prevalence of hepatitis C virus (HCV) infection and HCV genotypes of hemodialysis patients in Salvador, Northeastern Brazil. Brazilian Journal Of Medical And Biological Research 2006, 39: 595-602. PMID: 16648896, DOI: 10.1590/s0100-879x2006000500005.Peer-Reviewed Original ResearchConceptsHepatitis C virus infectionCandidate blood donorsC virus infectionHD patientsHCV infectionBlood donorsGenotype 1Virus infectionAnti-HCV seroprevalenceAnti-HCV prevalenceHCV genotype distributionAnti-HCV antibodiesHCV genotype 1Chronic liver diseaseTransfusion-transmitted infectionsCross-sectional studyDifferent dialysis centresHCV seroprevalenceChronic hemodialysisHemodialysis populationHCV RNAPositive patientsHemodialysis patientsHCV genotypesLiver disease
2001
Evaluation of Recombinant Leptospira Antigen-Based Enzyme-Linked Immunosorbent Assays for the Serodiagnosis of Leptospirosis
Flannery B, Costa D, Carvalho F, Guerreiro H, Matsunaga J, Da Silva E, Ferreira A, Riley L, Reis M, Haake D, Ko A. Evaluation of Recombinant Leptospira Antigen-Based Enzyme-Linked Immunosorbent Assays for the Serodiagnosis of Leptospirosis. Journal Of Clinical Microbiology 2001, 39: 3303-3310. PMID: 11526167, PMCID: PMC88335, DOI: 10.1128/jcm.39.9.3303-3310.2001.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, BacterialAntigens, BacterialEnzyme-Linked Immunosorbent AssayHumansImmunoglobulin GLeptospiraLeptospirosisLipoproteinsRecombinant ProteinsSensitivity and SpecificityConceptsEnzyme-linked immunosorbent assayIgG enzyme-linked immunosorbent assaySerodiagnosis of leptospirosisHealthy individualsImmunosorbent assayTest-positive individualsCases of leptospirosisHigh-incidence regionsLyme disease patientsMicroscopic agglutination testConvalescent phaseHepatitis patientsAcute phaseDengue patientsDisease patientsPatient seraHealthy residentsLeptospira antigensCutoff valueDiagnostic utilityPatientsAgglutination testUseful antigenLeptospirosisAntigen