2020
Role of GUCA1C in Primary Congenital Glaucoma and in the Retina: Functional Evaluation in Zebrafish
Morales-Cámara S, Alexandre-Moreno S, Bonet-Fernández J, Atienzar-Aroca R, Aroca-Aguilar J, Ferre-Fernández J, Méndez C, Morales L, Fernández-Sánchez L, Cuenca N, Coca-Prados M, Martínez-de-la-Casa J, Garcia-Feijoo J, Escribano J. Role of GUCA1C in Primary Congenital Glaucoma and in the Retina: Functional Evaluation in Zebrafish. Genes 2020, 11: 550. PMID: 32422965, PMCID: PMC7288452, DOI: 10.3390/genes11050550.Peer-Reviewed Original ResearchMeSH KeywordsAdultAmino Acid SequenceAnimalsApoptosisBase SequenceCRISPR-Cas SystemsFemaleGene EditingGene Knockout TechniquesGlaucomaGliosisGuanylate Cyclase-Activating ProteinsHigh-Throughput Nucleotide SequencingHumansMaleMiddle AgedPedigreeRetinaReverse Transcriptase Polymerase Chain ReactionSequence AlignmentSequence Homology, Amino AcidZebrafishZebrafish ProteinsConceptsPrimary congenital glaucomaCongenital glaucomaRetinal ganglion cell layerRetinal ganglion cell apoptosisCiliary epitheliumGlial fibrillary acidic proteinWhole-exome sequencing analysisGanglion cell layerGanglion cell apoptosisHuman ocular ciliary epitheliumFibrillary acidic proteinOcular anterior segmentIntraocular pressure regulationOcular ciliary epitheliumNon-pigmented ciliary epitheliumAutosomal recessive fashionOptical neuropathyOcular effectsRetinal damageMüller cellsAnterior segmentPressure regulationAcidic proteinKnockout animalsGuanylate cyclase
2015
LOXL1 gene variants and their association with pseudoexfoliation glaucoma (XFG) in Spanish patients
Álvarez L, García M, González-Iglesias H, Escribano J, Rodríguez-Calvo PP, Fernández-Vega L, Coca-Prados M. LOXL1 gene variants and their association with pseudoexfoliation glaucoma (XFG) in Spanish patients. BMC Medical Genomics 2015, 16: 72. PMID: 26319397, PMCID: PMC4593192, DOI: 10.1186/s12881-015-0221-y.Peer-Reviewed Original Research
2011
WDR36 and P53 Gene Variants and Susceptibility to Primary Open-Angle Glaucoma: Analysis of Gene-Gene Interactions
Blanco-Marchite C, Sánchez-Sánchez F, López-Garrido MP, Iñigez-de-Onzoño M, López-Martínez F, López-Sánchez E, Alvarez L, Rodríguez-Calvo PP, Méndez-Hernández C, Fernández-Vega L, García-Sánchez J, Coca-Prados M, García-Feijoo J, Escribano J. WDR36 and P53 Gene Variants and Susceptibility to Primary Open-Angle Glaucoma: Analysis of Gene-Gene Interactions. Investigative Ophthalmology & Visual Science 2011, 52: 8467-8478. PMID: 21931130, PMCID: PMC3208188, DOI: 10.1167/iovs.11-7489.Peer-Reviewed Original ResearchAgedAmino Acid SequenceBase SequenceCase-Control StudiesChromatography, High Pressure LiquidDNA Mutational AnalysisEpistasis, GeneticEye ProteinsFemaleGenetic Predisposition to DiseaseGenetic VariationGenotypeGlaucoma, Open-AngleHumansIntraocular PressureMaleMiddle AgedMolecular Sequence DataOcular HypertensionPolymerase Chain ReactionPolymorphism, Single NucleotideRegulatory Sequences, Nucleic AcidRisk FactorsSequence Analysis, DNATumor Suppressor Protein p53
2003
Production, purification, and functional analysis of recombinant human and mouse 17β-hydroxysteroid dehydrogenase type 7
Törn S, Nokelainen P, Kurkela R, Pulkka A, Menjivar M, Ghosh S, Coca-Prados M, Peltoketo H, Isomaa V, Vihko P. Production, purification, and functional analysis of recombinant human and mouse 17β-hydroxysteroid dehydrogenase type 7. Biochemical And Biophysical Research Communications 2003, 305: 37-45. PMID: 12732193, DOI: 10.1016/s0006-291x(03)00694-6.Peer-Reviewed Original ResearchConceptsPeripheral tissuesType 7Primary open-angle glaucomaEarly-onset prostate cancerOpen-angle glaucomaSex steroid hormonesIntracrine regulatorAdrenal glandProstate cancerEstrogenic milieuPotent androgenInactive metabolitesEstrogenic metabolitesSteroid metabolismPotent estrogenSteroid hormonesCancer 1DihydrotestosteroneRecombinant humanTissue distributionHormoneTissueHuman tissuesPresent studyMetabolic roleCRALBP transcriptional regulation in ciliary epithelial, retinal Müller and retinal pigment epithelial cells
Kennedy BN, Li C, Ortego J, Coca-Prados M, Sarthy VP, Crabb JW. CRALBP transcriptional regulation in ciliary epithelial, retinal Müller and retinal pigment epithelial cells. Experimental Eye Research 2003, 76: 257-260. PMID: 12565814, DOI: 10.1016/s0014-4835(02)00308-1.Peer-Reviewed Original ResearchConceptsTranscriptional regulationBinding Protein FunctionsCell-specific expressionPromoter analysisProtein functionRepressor elementEnhancer elementsPromoter constructsReporter activityRetinal pigment epithelial cellsPigment epithelial cellsCiliary epitheliumEpithelial cellsVisual cycleRPE cellsBPRegulationRetinal pigment epitheliumCellsMüller cellsGenesMutationsRLBP1 geneEpitheliumWildtype
1999
Identification, expression and chromosome localization of a human gene encoding a novel protein with similarity to the pilB family of transcriptional factors (pilin) and to bacterial peptide methionine sulfoxide reductases
Huang W, Escribano J, Sarfarazi M, Coca-Prados M. Identification, expression and chromosome localization of a human gene encoding a novel protein with similarity to the pilB family of transcriptional factors (pilin) and to bacterial peptide methionine sulfoxide reductases. Gene 1999, 233: 233-240. PMID: 10375640, DOI: 10.1016/s0378-1119(99)00131-6.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBacterial ProteinsBase SequenceChromosome MappingChromosomes, Human, Pair 10Ciliary BodyDNA, ComplementaryFluorescent Antibody Technique, IndirectHelicobacter pyloriHumansMethionine Sulfoxide ReductasesMicrofilament ProteinsMolecular Sequence DataOxidoreductasesPhylogenySequence Homology, Amino AcidStreptococcus pneumoniaeTranscription FactorsConceptsPeptide methionine sulfoxide reductaseCBS-1Ocular ciliary bodyChromosome localizationNovel proteinSignificant identitySulfoxide reductaseTranscription factorsCarboxyl terminusAmino acid sequence identityMammalian transcription factorsGlycine-rich proteinTerminal regionAmino acidsMethionine sulfoxide reductaseOpen reading frameRegulation of expressionAmino-terminal regionAmino acid sequenceCarboxyl-terminal regionMarkers WIIntergenic regionChromosome 10p12Human genesReading frameFunctional Expression of Components of the Natriuretic Peptide System in Human Ocular Nonpigmented Ciliary Epithelial Cells
Ortego J, Coca-Prados M. Functional Expression of Components of the Natriuretic Peptide System in Human Ocular Nonpigmented Ciliary Epithelial Cells. Biochemical And Biophysical Research Communications 1999, 258: 21-28. PMID: 10222228, DOI: 10.1006/bbrc.1999.0573.Peer-Reviewed Original ResearchConceptsNatriuretic peptide systemNPR-B receptorNPR-C receptorCiliary epithelial cellsReceptor mRNACiliary epitheliumEpithelial cellsNPE cellsCGMP responseAutocrine/paracrine modulatorPeptide systemHuman ocular ciliary epitheliumNonpigmented ciliary epithelial cellsOcular ciliary epitheliumCultured NPE cellsLong-term downregulationC-ANP4Paracrine modulatorEye pressureBNPActivation of PKCBasal levelsInhibitory effectRT-PCRReceptorsEffects of hypotonic swelling on the cellular distribution and expression of pICln in human nonpigmented ciliary epithelial cells
Sánchez-Torres J, Huang W, Civan M, Coca-Prados M. Effects of hypotonic swelling on the cellular distribution and expression of pICln in human nonpigmented ciliary epithelial cells. Current Eye Research 1999, 18: 408-416. PMID: 10435827, DOI: 10.1076/ceyr.18.6.408.5266.Peer-Reviewed Original ResearchConceptsOpen reading frameCiliary epithelial cellsPlasma membraneFusion proteinWestern blot analysisEpithelial cellsCellular distributionTag fusion proteinCandidate gene productsBlot analysisHuman nonpigmented ciliary epithelial cellsTotal cell extractsHypotonic treatmentHuman ciliary epithelial cellsMammalian cellsPolyclonal antibodiesReading frameGene productsNonpigmented ciliary epithelial cellsCultured ciliary epithelial cellsPerinuclear regionCellular localizationCell extractsCultured cellsProtein
1997
Cloning and characterization of subtracted cDNAs from a human ciliary body library encoding TIGR, a protein involved in juvenile open angle glaucoma with homology to myosin and olfactomedin
Ortego J, Escribano J, Coca-Prados M. Cloning and characterization of subtracted cDNAs from a human ciliary body library encoding TIGR, a protein involved in juvenile open angle glaucoma with homology to myosin and olfactomedin. FEBS Letters 1997, 413: 349-353. PMID: 9280311, DOI: 10.1016/s0014-5793(97)00934-4.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceCiliary BodyCloning, MolecularCytoskeletal ProteinsDNA, ComplementaryExtracellular Matrix ProteinsEyeEye ProteinsGene LibraryGlaucoma, Open-AngleGlycoproteinsHumansMolecular Sequence DataMyosinsOrgan SpecificityRNA, MessengerSequence Homology, Amino AcidSequence Homology, Nucleic Acid
1996
Association of ClC-3 Channel with Cl− Transport by Human Nonpigmented Ciliary Epithelial Cells
Coca-Prados M, Sánchez-Torres J, Peterson-Yantorno K, Civan M. Association of ClC-3 Channel with Cl− Transport by Human Nonpigmented Ciliary Epithelial Cells. The Journal Of Membrane Biology 1996, 150: 197-208. PMID: 8661780, DOI: 10.1007/s002329900044.Peer-Reviewed Original ResearchIdentification of a Neuropeptide and Neuropeptide‐Processing Enzymes in Aqueous Humor Confers Neuroendocrine Features to the Human Ocular Ciliary Epithelium
Ortego J, Escribano J, Crabb J, Coca‐Prados M. Identification of a Neuropeptide and Neuropeptide‐Processing Enzymes in Aqueous Humor Confers Neuroendocrine Features to the Human Ocular Ciliary Epithelium. Journal Of Neurochemistry 1996, 66: 787-796. PMID: 8592153, DOI: 10.1046/j.1471-4159.1996.66020787.x.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAnimalsAqueous HumorBase SequenceCarboxypeptidase HCarboxypeptidasesCell LineChild, PreschoolChromograninsCiliary BodyEpithelial CellsEpitheliumFluorescent Antibody Technique, IndirectHumansMixed Function OxygenasesMolecular Sequence DataMultienzyme ComplexesNeurosecretory SystemsProteinsRatsConceptsNeuropeptide processing enzymesCarboxypeptidase ECiliary epithelial cellsOcular ciliary epitheliumSecretogranin IICell-cell communicationEpithelial cellsHuman ocular ciliary epitheliumCDNA clonesCell signalingNonpigmented ciliary epithelial cellsCultured ciliary epithelial cellsProcessing enzymesMolecular markersPeptidylglycine alphaPhorbol esterSpecialized groupSpecific probesMammalian eyeNeuroendocrine tissuesEnzymeCiliary epitheliumKey functionsCellsPolymerase chain reaction
1995
Cell-specific expression of the human Na+,K(+)-ATPase beta 2 subunit isoform in the nonpigmented ciliary epithelium.
Coca-Prados M, Fernández-Cabezudo M, Sánchez-Torres J, Crabb J, Ghosh S. Cell-specific expression of the human Na+,K(+)-ATPase beta 2 subunit isoform in the nonpigmented ciliary epithelium. Investigative Ophthalmology & Visual Science 1995, 36: 2717-28. PMID: 7499094.Peer-Reviewed Original ResearchConceptsBeta 2 isoformNonpigmented ciliary epithelial cellsOcular tissuesCiliary epitheliumBeta 1NPE cell linesNonpigmented ciliary epitheliumNPE cellsHuman ocular tissuesSubunit isoformsBlot analysisATPase subunit isoformsSpecific anti-peptide antibodiesRestricted cellular distributionPolymerase chain reactionCiliary epithelial cellsBeta-subunit isoformsAlpha-subunit isoformsAnti-peptide antibodiesODM-2Polymerase chain reaction amplificationChain reaction amplificationHuman ciliary epitheliumCell-specific distributionPattern of expressionIsolation and Characterization of Cell-Specific cDNA Clones from a Subtractive Library of the Ocular Ciliary Body of a Single Normal Human Donor: Transcription and Synthesis of Plasma Proteins1
Escribano J, Ortego J, Coca-Prados M. Isolation and Characterization of Cell-Specific cDNA Clones from a Subtractive Library of the Ocular Ciliary Body of a Single Normal Human Donor: Transcription and Synthesis of Plasma Proteins1. The Journal Of Biochemistry 1995, 118: 921-931. PMID: 8749308, DOI: 10.1093/jb/118.5.921.Peer-Reviewed Original ResearchConceptsSubtractive cDNA libraryOcular ciliary bodyCDNA clonesCDNA libraryCell-restricted expressionPartial DNA sequencesCell-specific genesPotential candidate genesSubtractive libraryComplement component C4Significant homologyHomology searchProtein databaseDNA sequencesCiliary bodyCandidate genesTranscriptional expressionNorthern hybridizationSignificant genesGenesIntraocular pressureCiliary epithelial cellsPlasma proteinsProteinVascular endothelial cellsPKC-sensitive Cl- channels associated with ciliary epithelial homologue of pICln
Coca-Prados M, Anguita J, Chalfant M, Civan M. PKC-sensitive Cl- channels associated with ciliary epithelial homologue of pICln. American Journal Of Physiology 1995, 268: c572-c579. PMID: 7534980, DOI: 10.1152/ajpcell.1995.268.3.c572.Peer-Reviewed Original ResearchMeSH KeywordsAlkaloidsAnimalsBase SequenceBlotting, NorthernCells, CulturedChild, PreschoolChloride ChannelsCiliaCystic Fibrosis Transmembrane Conductance RegulatorElectric ConductivityEpitheliumHumansIon ChannelsKineticsMembrane ProteinsMolecular Sequence DataPolymerase Chain ReactionProtein Kinase CRatsRNA, MessengerStaurosporine
1994
Nucleotide sequence of a cDNA for the β2 subunit isoform of Na+, K+-ATPase from human retina
Hernando N, Martin-Vasallo P, Ghosh S, Ghosh P, Swaroop A, Coca-Prados M. Nucleotide sequence of a cDNA for the β2 subunit isoform of Na+, K+-ATPase from human retina. Biochimica Et Biophysica Acta 1994, 1189: 109-111. PMID: 8305453, DOI: 10.1016/0005-2736(94)90287-9.Peer-Reviewed Original Research
1993
Cloning of the Bovine Plasma Selenium-Dependent Glutathione Peroxidase (GP) cDNA from the Ocular Ciliary Epithelium: Expression of the Plasma and Cellular Forms within the Mammalian Eye1
Martín-Alonso J, Ghosh S, Coca-Prados M. Cloning of the Bovine Plasma Selenium-Dependent Glutathione Peroxidase (GP) cDNA from the Ocular Ciliary Epithelium: Expression of the Plasma and Cellular Forms within the Mammalian Eye1. The Journal Of Biochemistry 1993, 114: 284-291. PMID: 8262911, DOI: 10.1093/oxfordjournals.jbchem.a124168.Peer-Reviewed Original ResearchConceptsOcular ciliary epitheliumGlutathione peroxidase cDNAMammalian eyeCorresponding amino acid sequencesDifferential gene expressionOpen reading frameAmino acid sequenceBovine glutathione peroxidasePeroxidase cDNAMolecular cloningOverall identityReading frameHuman GPxAcid sequenceGene expressionCellular formCiliary epitheliumFunctional roleAmino acidsTranscriptsOxidative damageGlutathione peroxidaseCloningOxidative stressExpressionDifferential Expression of the Cellular Retinaldehyde-binding Protein in Bovine Ciliary Epithelium
Martı̀n-Alonso J, Ghosh S, Hernando N, Crabb J, Coca-Prados M. Differential Expression of the Cellular Retinaldehyde-binding Protein in Bovine Ciliary Epithelium. Experimental Eye Research 1993, 56: 659-669. PMID: 8595808, DOI: 10.1006/exer.1993.1083.Peer-Reviewed Original Research
1992
Isolation of a cDNA encoding a glutathione S-transferase (GST) class-π from the bovine ocular ciliary epithelium
Hernando N, Martin-Alonso J, Ghosh S, Coca-Prados M. Isolation of a cDNA encoding a glutathione S-transferase (GST) class-π from the bovine ocular ciliary epithelium. Experimental Eye Research 1992, 55: 711-718. PMID: 1478280, DOI: 10.1016/0014-4835(92)90175-r.Peer-Reviewed Original ResearchMolecular Cloning of the Bovine CD9 Antigen from Ocular Ciliary Epithelial Cells1
Martín-Alonso J, Hernando N, Ghosh S, Coca-Prados M. Molecular Cloning of the Bovine CD9 Antigen from Ocular Ciliary Epithelial Cells1. The Journal Of Biochemistry 1992, 112: 63-67. PMID: 1339429, DOI: 10.1093/oxfordjournals.jbchem.a123866.Peer-Reviewed Original Research