2020
Platform Effects on Regeneration by Pulmonary Basal Cells as Evaluated by Single-Cell RNA Sequencing
Greaney AM, Adams TS, Raredon M, Gubbins E, Schupp JC, Engler AJ, Ghaedi M, Yuan Y, Kaminski N, Niklason LE. Platform Effects on Regeneration by Pulmonary Basal Cells as Evaluated by Single-Cell RNA Sequencing. Cell Reports 2020, 30: 4250-4265.e6. PMID: 32209482, PMCID: PMC7175071, DOI: 10.1016/j.celrep.2020.03.004.Peer-Reviewed Original ResearchConceptsSingle-cell RNA sequencingBasal marker expressionBasal cellsChronic pulmonary diseaseRat tracheal epitheliumPulmonary diseaseRNA sequencingCell-based therapiesRat tracheaAir-liquid interfaceTissue graftMarker expressionTracheal epitheliumRegenerative outcomesTracheaEpithelial progenitorsDifferential outcomesEpitheliumOutcomesWhole organPopulation level
2019
Engineering of human brain organoids with a functional vascular-like system
Cakir B, Xiang Y, Tanaka Y, Kural MH, Parent M, Kang YJ, Chapeton K, Patterson B, Yuan Y, He CS, Raredon MSB, Dengelegi J, Kim KY, Sun P, Zhong M, Lee S, Patra P, Hyder F, Niklason LE, Lee SH, Yoon YS, Park IH. Engineering of human brain organoids with a functional vascular-like system. Nature Methods 2019, 16: 1169-1175. PMID: 31591580, PMCID: PMC6918722, DOI: 10.1038/s41592-019-0586-5.Peer-Reviewed Original ResearchConceptsHuman cortical organoidsBlood-brain barrier characteristicsTrans-endothelial electrical resistanceVasculature-like structuresHuman brain organoidsHuman brain developmentCortical organoidsFunctional maturationPrenatal brainBrain diseasesBrain developmentHuman embryonic stem cellsBlood vesselsBrain organoidsTight junctionsDiseaseStem cellsOrganoidsVariant 2Nutrient transportersNutrient deliveryCellsEndotheliumMicrovasculatureNon-invasive and real-time measurement of microvascular barrier in intact lungs
Engler AJ, Raredon MSB, Le AV, Yuan Y, Oczkowicz YA, Kan EL, Baevova P, Niklason LE. Non-invasive and real-time measurement of microvascular barrier in intact lungs. Biomaterials 2019, 217: 119313. PMID: 31280072, PMCID: PMC6863174, DOI: 10.1016/j.biomaterials.2019.119313.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBioreactorsComputer SystemsEndotheliumLungLung InjuryMaleMicrovesselsRats, Sprague-DawleyTissue Culture TechniquesTissue EngineeringEpac agonist improves barrier function in iPSC-derived endothelial colony forming cells for whole organ tissue engineering
Yuan Y, Engler AJ, Raredon MS, Le A, Baevova P, Yoder MC, Niklason LE. Epac agonist improves barrier function in iPSC-derived endothelial colony forming cells for whole organ tissue engineering. Biomaterials 2019, 200: 25-34. PMID: 30754017, DOI: 10.1016/j.biomaterials.2019.02.005.Peer-Reviewed Original Research
2017
Lung regeneration
Calle EA, Leiby KL, Raredon MB, Niklason LE. Lung regeneration. Current Opinion In Anaesthesiology 2017, 30: 23-29. PMID: 27922848, PMCID: PMC5283945, DOI: 10.1097/aco.0000000000000425.Peer-Reviewed Original Research
2014
A Call to Craft
Raredon MS, Niklason LE. A Call to Craft. Science Translational Medicine 2014, 6: 218fs1. PMID: 24401937, PMCID: PMC4122270, DOI: 10.1126/scitranslmed.3008229.Commentaries, Editorials and LettersConceptsTissue engineers
2010
Tissue-Engineered Lungs for in Vivo Implantation
Petersen TH, Calle EA, Zhao L, Lee EJ, Gui L, Raredon MB, Gavrilov K, Yi T, Zhuang ZW, Breuer C, Herzog E, Niklason LE. Tissue-Engineered Lungs for in Vivo Implantation. Science 2010, 329: 538-541. PMID: 20576850, PMCID: PMC3640463, DOI: 10.1126/science.1189345.Peer-Reviewed Original ResearchConceptsLung tissueLung matrixAcellular lung matrixNative lung tissueTissue-engineered lungsLung transplantationPrimary therapyAdult lung tissueAdult ratsPulmonary epitheliumVascular endotheliumFunctional lungLung regenerationVascular compartmentLungSeeded endothelial cellsMechanical characteristicsEndothelial cellsVivo implantationRatsEpitheliumTissueCellular componentsExtracellular matrixGas exchange