Featured Publications
Comparison of percutaneous vs oral infection of hamsters with the hookworm Ancylostoma ceylanicum: Parasite development, pathology and primary immune response
Bungiro RD, Harrison LM, Dondji B, Cappello M. Comparison of percutaneous vs oral infection of hamsters with the hookworm Ancylostoma ceylanicum: Parasite development, pathology and primary immune response. PLOS Neglected Tropical Diseases 2022, 16: e0010098. PMID: 34986139, PMCID: PMC8765627, DOI: 10.1371/journal.pntd.0010098.Peer-Reviewed Original ResearchMeSH KeywordsAncylostomaAncylostomiasisAnimalsCricetinaeDisease Models, AnimalHemoglobinsMaleMesocricetusMouthSkinConceptsCellular immune responsesGolden Syrian hamstersImmune responsePercutaneous infectionOral infectionSyrian hamstersAncylostoma ceylanicumPrimary immune responseRoute of infectionModerate growth delayFaecal egg outputPercutaneous groupImmunological manifestationsAntibody titersHookworm Ancylostoma ceylanicumInfected hamstersWorm recoveryLarval antigensBlood hemoglobinDisease pathogenesisHost responseGrowth delayHuman infectionsInfectionA. ceylanicum
2016
Hamsters vaccinated with Ace-mep-7 DNA vaccine produced protective immunity against Ancylostoma ceylanicum infection
Wiśniewski M, Jaros S, Bąska P, Cappello M, Długosz E, Wędrychowicz H. Hamsters vaccinated with Ace-mep-7 DNA vaccine produced protective immunity against Ancylostoma ceylanicum infection. Experimental Parasitology 2016, 163: 1-7. PMID: 26795262, DOI: 10.1016/j.exppara.2016.01.006.Peer-Reviewed Original ResearchConceptsA. ceylanicum infectionAncylostoma ceylanicum infectionHookworm speciesWorm burden reductionsSyrian golden hamstersProtective immunityDNA vaccineGut mucosaGastrointestinal tractIntestinal nematodesGolden hamstersBurden reductionAdult wormsHemoglobin digestionEgg countsImmunizationVaccineInfectionHamstersMetalloproteasesMucosaHookwormBloodTractImmunity
2014
Molecular cloning and characterization of a nematode polyprotein antigen/allergen from the human and animal hookworm Ancylostoma ceylanicum
Fairfax KC, Harrison LM, Cappello M. Molecular cloning and characterization of a nematode polyprotein antigen/allergen from the human and animal hookworm Ancylostoma ceylanicum. Molecular And Biochemical Parasitology 2014, 198: 37-44. PMID: 25481749, PMCID: PMC4354846, DOI: 10.1016/j.molbiopara.2014.11.005.Peer-Reviewed Original ResearchConceptsAmino acid sequenceFatty acids de novoShares sequence identityExcretory/secretory proteinsPseudocoelomic spaceMolecular cloningCis-parinaric acidPartial cDNASequence identityAcid sequenceFatty acidsSimilar proteinsSecretory proteinsHookworm biologyRecombinant subunitsSoluble adult worm extractProteinAntigens/allergensSubunits
2013
In vitro Screening of Compounds against Laboratory and Field Isolates of Human Hookworm Reveals Quantitative Differences in Anthelmintic Susceptibility
Treger RS, Otchere J, Keil MF, Quagraine JE, Rai G, Mott BT, Humphries DL, Wilson M, Cappello M, Vermeire JJ. In vitro Screening of Compounds against Laboratory and Field Isolates of Human Hookworm Reveals Quantitative Differences in Anthelmintic Susceptibility. American Journal Of Tropical Medicine And Hygiene 2013, 90: 71-74. PMID: 24297811, PMCID: PMC3886431, DOI: 10.4269/ajtmh.12-0547.Peer-Reviewed Original ResearchConceptsField isolatesA. ceylanicumHookwormScreening of compoundsAncylostoma ceylanicumHuman hookwormAnthelmintic compoundsLaboratory strainsHookworm speciesPercent inhibitionSchool childrenTwofold increaseSelect compoundsAnthelmintic activityNovel anthelmintic compoundsAnthelmintic susceptibilityBrong Ahafo RegionIsolatesAhafo RegionAncylostoma ceylanicum metalloprotease 6 DNA vaccination induces partial protection against hookworm challenge infection
Wiśniewski M, Jaros S, Bąska P, Cappello M, Wędrychowicz H. Ancylostoma ceylanicum metalloprotease 6 DNA vaccination induces partial protection against hookworm challenge infection. Acta Parasitologica 2013, 58: 376-383. PMID: 23990436, DOI: 10.2478/s11686-013-0151-9.Peer-Reviewed Original ResearchConceptsChallenge infectionAnthelminthic drug resistanceEffective human vaccineWorm burden reductionsGroups of hamstersIron deficiency anemiaCDNA vaccineTriple immunizationInfected childrenDeficiency anemiaDNA vaccineHookworm infectionDNA vaccinationHuman vaccinesAnimal modelsNumerous antigensVaccine developmentGrowth retardationIntestinal nematodesDrug resistanceVaccineBurden reductionPartial protectionInfectionHemoglobin digestionPeroxiredoxin-1 from the Human Hookworm Ancylostoma ceylanicum Forms a Stable Oxidized Decamer and Is Covalently Inhibited by Conoidin A
Nguyen JB, Pool CD, Wong CY, Treger RS, Williams DL, Cappello M, Lea WA, Simeonov A, Vermeire JJ, Modis Y. Peroxiredoxin-1 from the Human Hookworm Ancylostoma ceylanicum Forms a Stable Oxidized Decamer and Is Covalently Inhibited by Conoidin A. Cell Chemical Biology 2013, 20: 991-1001. PMID: 23891152, PMCID: PMC3755041, DOI: 10.1016/j.chembiol.2013.06.011.Peer-Reviewed Original ResearchCloning and molecular characterization of cDNAs encoding three Ancylostoma ceylanicum secreted proteins
Siwińska A, Bąska P, Daniłowicz-Luebert E, Januszkiewicz K, Długosz E, Wędrychowicz H, Cappello M, Wiśniewski M. Cloning and molecular characterization of cDNAs encoding three Ancylostoma ceylanicum secreted proteins. Acta Parasitologica 2013, 58: 112-118. PMID: 23377920, DOI: 10.2478/s11686-013-0116-z.Peer-Reviewed Original Research
2012
Frequency and intensity of exposure mediate resistance to experimental infection with the hookworm, Ancylostoma ceylanicum
Davey D, Manickam N, Simms BT, Harrison LM, Vermeire JJ, Cappello M. Frequency and intensity of exposure mediate resistance to experimental infection with the hookworm, Ancylostoma ceylanicum. Experimental Parasitology 2012, 133: 243-249. PMID: 23232252, PMCID: PMC3580025, DOI: 10.1016/j.exppara.2012.11.010.Peer-Reviewed Original ResearchConceptsExperimental infectionThird-stage hookworm larvaeParasite-specific IgAHumoral immune responseStudy of pathogenesisResource-limited countriesHuman hookworm diseaseHookworm infectionLifelong susceptibilityPrimary infectionImmune responseProlonged susceptibilitySubsequent challengeAntibody productionVaccine developmentHookworm larvaeHookworm diseaseIntestinal nematodesInfectionPathological effectsRepeated exposureAncylostoma ceylanicumContinued susceptibilityMajor causeMediate resistance
2011
Ancylostoma ceylanicum Excretory–Secretory Protein 2 Adopts a Netrin-Like Fold and Defines a Novel Family of Nematode Proteins
Kucera K, Harrison LM, Cappello M, Modis Y. Ancylostoma ceylanicum Excretory–Secretory Protein 2 Adopts a Netrin-Like Fold and Defines a Novel Family of Nematode Proteins. Journal Of Molecular Biology 2011, 408: 9-17. PMID: 21352830, PMCID: PMC3070796, DOI: 10.1016/j.jmb.2011.02.033.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAncylostomaAncylostomatoideaAncylostomiasisAnimalsComplement System ProteinsHelminth ProteinsHumansMatrix MetalloproteinasesMolecular Sequence DataNerve Growth FactorsNetrin-1Protein ConformationProtein FoldingSequence Homology, Amino AcidTissue Inhibitor of MetalloproteinasesTumor Suppressor ProteinsConceptsMatrix metalloproteasesHost immune responseComplement factor C3Nematode proteinsProtein 2Complement-mediated cell lysisHookworm anaemiaMucosal vaccinesImmune responseUseful model organismFactor C3Decoy receptorDisease pathogenesisTissue inhibitorIntestinal attachmentNovel familyAncylostoma ceylanicumImmunoreactive moleculesAdult wormsGlobal healthModel organismsHookwormSequence homologyÅ resolutionHuman parasites
2010
CD4+ T cells mediate mucosal and systemic immune responses to experimental hookworm infection
DONDJI B, SUN T, BUNGIRO RD, VERMEIRE JJ, HARRISON LM, BIFULCO C, CAPPELLO M. CD4+ T cells mediate mucosal and systemic immune responses to experimental hookworm infection. Parasite Immunology 2010, 32: 406-413. PMID: 20500671, PMCID: PMC3241967, DOI: 10.1111/j.1365-3024.2010.01204.x.Peer-Reviewed Original ResearchConceptsT cellsImmune responseHookworm infectionHost cellular immune responseExperimental hookworm infectionT-cell depletionMesenteric lymph nodesSystemic immune responsesCellular immune responsesHumoral immune responseIntestinal nematode infectionsIntestinal worm burdenResource-limited countriesHookworm antigensLymph nodesSevere anemiaIntraperitoneal injectionLymphocyte proliferationHookworm Ancylostoma ceylanicumIsotype controlDisease pathogenesisWorm burdenSyrian hamstersAncylostoma ceylanicumEffector role
2009
Characterisation of a fatty acid and retinol binding protein orthologue from the hookworm Ancylostoma ceylanicum
Fairfax KC, Vermeire JJ, Harrison LM, Bungiro RD, Grant W, Husain SZ, Cappello M. Characterisation of a fatty acid and retinol binding protein orthologue from the hookworm Ancylostoma ceylanicum. International Journal For Parasitology 2009, 39: 1561-1571. PMID: 19591834, PMCID: PMC2760681, DOI: 10.1016/j.ijpara.2009.06.005.Peer-Reviewed Original ResearchConceptsMucosal adjuvant cholera toxinAdjuvant cholera toxinCause of anemiaLive adult wormsIntestinal worm burdenFatty acidsResource limited countriesFatty acid uptakeHookworm Ancylostoma ceylanicumReal-time PCRFatty acids de novoWorm burdenVaccine developmentLimited countriesIntestinal nematodesEx vivoA. ceylanicumCholera toxinHookworm biologyIndividual fatty acidsAncylostoma ceylanicumAcid uptakeAdult wormsProtein 1Potential role
2008
Mucosal antibody responses in experimental hookworm infection
BUNGIRO RD, SUN T, HARRISON LM, SHOEMAKER CB, CAPPELLO M. Mucosal antibody responses in experimental hookworm infection. Parasite Immunology 2008, 30: 293-303. PMID: 18312503, DOI: 10.1111/j.1365-3024.2008.01023.x.Peer-Reviewed Original ResearchConceptsMucosal IgA responsesSystemic immune responsesIgA responsesImmune responseHookworm infectionInfected hamstersRobust systemic immune responseAncylostoma ceylanicum infectionExperimental hookworm infectionFecal IgA responsesParasite-specific IgAMucosal immune responsesMucosal antibody responsesIntestinal worm burdenExcretory-secretory productsMucosal antibodiesProtective immunityChallenge infectionSerum IgGAntibody responsePrimary infectionSecretory IgAHamster modelIntestinal mucosaWorm burdenRole for Nitric Oxide in Hookworm-Associated Immune Suppression
Dondji B, Bungiro RD, Harrison LM, Vermeire JJ, Bifulco C, McMahon-Pratt D, Cappello M. Role for Nitric Oxide in Hookworm-Associated Immune Suppression. Infection And Immunity 2008, 76: 2560-2567. PMID: 18347036, PMCID: PMC2423093, DOI: 10.1128/iai.00094-08.Peer-Reviewed Original ResearchConceptsAntigen-presenting cellsHookworm infectionNitric oxideInfected animalsMesenteric lymph node cellsHost cellular immune responseCellular immune responsesLymph node cellsProliferative capacityT cell preparationsSurface immunoglobulin GParasite-induced immunosuppressionResource-poor countriesHookworm antigensMLN cellsLymphocyte subpopulationsPositive lymphocytesCellular immunityImmune suppressionLymphocyte proliferationNode cellsFluorescence-activated cell sortingInfected hamstersImmune responseAnimal studies
2007
Short report: an agar plate method for culturing hookworm larvae: analysis of growth kinetics and infectivity compared with standard coproculture techniques.
Reiss D, Harrison LM, Bungiro R, Cappello M. Short report: an agar plate method for culturing hookworm larvae: analysis of growth kinetics and infectivity compared with standard coproculture techniques. American Journal Of Tropical Medicine And Hygiene 2007, 77: 1087-90. PMID: 18165527, DOI: 10.4269/ajtmh.2007.77.1087.Peer-Reviewed Original ResearchStructural and Functional Characterization of a Secreted Hookworm Macrophage Migration Inhibitory Factor (MIF) That Interacts with the Human MIF Receptor CD74*
Cho Y, Jones BF, Vermeire JJ, Leng L, DiFedele L, Harrison LM, Xiong H, Kwong YK, Chen Y, Bucala R, Lolis E, Cappello M. Structural and Functional Characterization of a Secreted Hookworm Macrophage Migration Inhibitory Factor (MIF) That Interacts with the Human MIF Receptor CD74*. Journal Of Biological Chemistry 2007, 282: 23447-23456. PMID: 17567581, PMCID: PMC3707627, DOI: 10.1074/jbc.m702950200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAncylostomaAnimalsAntigens, Differentiation, B-LymphocyteCricetinaeCytokinesHistocompatibility Antigens Class IIHumansIntestinal MucosaMacrophage Migration-Inhibitory FactorsMesocricetusMolecular Sequence DataProtein BindingRabbitsRecombinant ProteinsSequence Homology, Amino AcidConceptsMacrophage migration inhibitory factorMIF receptor CD74Migration inhibitory factorReceptor CD74Inhibitory factorHost immune responseHuman macrophage migration inhibitory factorMIF proteinHookworm Ancylostoma ceylanicumImmune responseDisease pathogenesisHuman cytokinesMacrophage migrationAncylostoma ceylanicumMajor causeWorm survivalParasite survivalISO-1Significant differencesTautomerase activityHookworm proteinsCytokinesCD74HookwormRelative bioactivity
2006
Molecular cloning and characterization of a C-type lectin from Ancylostoma ceylanicum: Evidence for a role in hookworm reproductive physiology
Brown AC, Harrison LM, Kapulkin W, Jones BF, Sinha A, Savage A, Villalon N, Cappello M. Molecular cloning and characterization of a C-type lectin from Ancylostoma ceylanicum: Evidence for a role in hookworm reproductive physiology. Molecular And Biochemical Parasitology 2006, 151: 141-147. PMID: 17129620, PMCID: PMC1831819, DOI: 10.1016/j.molbiopara.2006.10.017.Peer-Reviewed Original ResearchConceptsC-type lectinRecombinant proteinsAmino acid mature proteinDrosophila S2 cellsPutative C-type lectinReproductive physiologySecretory signal sequenceEssential cell functionsSoluble protein extractsEgg cell membraneS2 cellsMature proteinCDNA correspondingProtein familyCDNA correspondsSignal sequenceMolecular cloningSperm proteinsRelated proteinsReverse transcription-PCRProtein extractsCell membraneProteinTranscription-PCRCell functionA purified Bacillus thuringiensis crystal protein with therapeutic activity against the hookworm parasite Ancylostoma ceylanicum
Cappello M, Bungiro RD, Harrison LM, Bischof LJ, Griffitts JS, Barrows BD, Aroian RV. A purified Bacillus thuringiensis crystal protein with therapeutic activity against the hookworm parasite Ancylostoma ceylanicum. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 15154-15159. PMID: 17005719, PMCID: PMC1622792, DOI: 10.1073/pnas.0607002103.Peer-Reviewed Original ResearchConceptsCry proteinsCrystal proteinsSoil bacterium Bacillus thuringiensisBacterium Bacillus thuringiensisBacillus thuringiensis crystal proteinsBt Cry proteinsVivo anthelminthic activityFree-living nematodesA. ceylanicumLarval development assaysAdult A. ceylanicumGastrointestinal nematodesBacillus thuringiensisParasitic nematodesNematode parasitesVeterinary useCry5BAncylostoma ceylanicumNematodesDevelopment assaysEgg excretionAdult female wormsHuman diseasesWorm burdenPermissive hostDietary Iron Content Mediates Hookworm Pathogenesis In Vivo
Held MR, Bungiro RD, Harrison LM, Hamza I, Cappello M. Dietary Iron Content Mediates Hookworm Pathogenesis In Vivo. Infection And Immunity 2006, 74: 289-295. PMID: 16368983, PMCID: PMC1346670, DOI: 10.1128/iai.74.1.289-295.2006.Peer-Reviewed Original ResearchMeSH KeywordsAncylostomaAncylostomiasisAnimalsCricetinaeGenetic Predisposition to DiseaseIron, DietaryMaleConceptsDay 20 postinfectionGrowth delayIron-restricted dietHigh-iron dietIntestinal worm burdenDietary iron restrictionIron deficiency anemiaBlood hemoglobin levelsDietary iron contentGreater weight lossSignificant growth delayHemoglobin levelsDeficiency anemiaHookworm infectionDiet groupHamster modelSevere diseaseStandard dietUninfected controlsHemoglobin concentrationUninfected animalsWorm burdenDay 10Infected animalsHookworm disease
2005
Detection of excretory/secretory coproantigens in experimental hookworm infection.
Bungiro RD, Cappello M. Detection of excretory/secretory coproantigens in experimental hookworm infection. American Journal Of Tropical Medicine And Hygiene 2005, 73: 915-20. PMID: 16282303, DOI: 10.4269/ajtmh.2005.73.915.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayMolecular host-parasite interactionsFuture diagnostic methodsExcretory/secretoryFecal extractsImmunosorbent assayA. ceylanicum infectionExperimental hookworm infectionIntestinal worm burdenRabbit polyclonal IgG antibodyPolyclonal IgG antibodiesES proteinsDiagnostic methodsHookworm antigensDetectionHookworm infectionIgG antibodiesStool samplesWorm burdenDay 17Hamster serumCoproantigenFaecal eggMug/Degrees C.
2004
Hookworm infection: new developments and prospects for control
Bungiro R, Cappello M. Hookworm infection: new developments and prospects for control. Current Opinion In Infectious Diseases 2004, 17: 421-426. PMID: 15353961, DOI: 10.1097/00001432-200410000-00006.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsHookworm infectionHookworm diseaseEffective global controlPathological immune responsesMajor health burdenParasite virulence factorsNew chemotherapeutic agentsDevelopment of vaccinesHookworm anaemiaImmunoepidemiological studiesMass chemotherapyHealth burdenImmune responseMolecular pathogenesisGrowth delayChemotherapeutic agentsInfectionVaccineSocioeconomic statusDiseaseVirulence factorsGlobal healthBenzimidazole anthelminthicsPathogenesisAnthelminthic resistance