2011
Protein Phosphatase 2A Interacts with the Na+,K+-ATPase and Modulates Its Trafficking by Inhibition of Its Association with Arrestin
Kimura T, Han W, Pagel P, Nairn AC, Caplan MJ. Protein Phosphatase 2A Interacts with the Na+,K+-ATPase and Modulates Its Trafficking by Inhibition of Its Association with Arrestin. PLOS ONE 2011, 6: e29269. PMID: 22242112, PMCID: PMC3248462, DOI: 10.1371/journal.pone.0029269.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArrestinBinding, CompetitiveChlorocebus aethiopsCOS CellsGene DeletionG-Protein-Coupled Receptor KinasesHumansImmunoprecipitationKidneyMicePhosphorylationProtein BindingProtein BiosynthesisProtein Phosphatase 2Protein Structure, SecondaryProtein SubunitsProtein TransportRatsSodium-Potassium-Exchanging ATPaseConceptsC subunitATPase traffickingCatalytic subunitP-type ATPase familyG proteinsCatalytic C subunitTwo-hybrid systemIon transport proteinsEffect of arrestinNative rat kidneyATPase interactsProtein phosphataseATPase familyReceptor kinaseHomologous sequencesTransport proteinsFunctional domainsTrafficking propertiesImportant regulatorArrestinReceptor signalingIon pumpsTraffickingDirect interactionPP2APreactivation of AMPK by metformin may ameliorate the epithelial cell damage caused by renal ischemia
Seo-Mayer PW, Thulin G, Zhang L, Alves DS, Ardito T, Kashgarian M, Caplan MJ. Preactivation of AMPK by metformin may ameliorate the epithelial cell damage caused by renal ischemia. American Journal Of Physiology. Renal Physiology 2011, 301: f1346-f1357. PMID: 21849490, PMCID: PMC3233870, DOI: 10.1152/ajprenal.00420.2010.Peer-Reviewed Original ResearchConceptsEpithelial cell polarityMDCK cellsPlasma membrane domainsIon transport proteinsEpithelial cell organizationCellular energy sensorAMPK activator metforminMadin-Darby canine kidney cellsBasolateral plasma membraneShort hairpin RNACanine kidney cellsCell polarityImmunofluoresence localizationRenal epithelial cellsMembrane domainsNa-K-ATPaseProtein kinaseAMPK activatorPlasma membraneVesicular compartmentsAMPK activityTransport proteinsEnergy sensorMolecular consequencesBasolateral localization
2007
Tetraspan proteins: regulators of renal structure and function
Caplan MJ, Kamsteeg EJ, Duffield A. Tetraspan proteins: regulators of renal structure and function. Current Opinion In Nephrology & Hypertension 2007, 16: 353-358. PMID: 17565278, DOI: 10.1097/mnh.0b013e328177b1fa.Peer-Reviewed Original ResearchConceptsProtein partnersRenal ion transport proteinsIon transport proteinsModulates cell adhesionImportant physiological ramificationsNormal glomerular architecturePartner proteinsTissue morphogenesisMembrane proteinsEndocytic internalizationTransport proteinsPhysiological processesRegulatory rolePhysiological ramificationsTransporter functionCell adhesionIntegrin moleculesTetraspan familyProteinEpithelial cellsTetraspansGlomerular architectureNovel classFunctional propertiesCellsThe Future of the Pump
Caplan MJ. The Future of the Pump. Journal Of Clinical Gastroenterology 2007, 41: s217-s222. PMID: 17575526, DOI: 10.1097/mcg.0b013e31803233da.Peer-Reviewed Original ResearchConceptsIon transport proteinsLarge macromolecular complexesMacromolecular complexesGastric parietal cellsTransport proteinsSecond messengerMacromolecular interactionsIon translocationRegulatory processesK-ATPaseCritical roleTight controlX-ray crystallographic techniquesParietal cellsCrystallographic techniquesCellsKinasePharmacologic suppressionTraffickingProteinTranslocationMessengerMolecular structureRegulationSecretion
2003
TRANSPORT PROTEIN TRAFFICKING IN POLARIZED CELLS
Muth TR, Caplan MJ. TRANSPORT PROTEIN TRAFFICKING IN POLARIZED CELLS. Annual Review Of Cell And Developmental Biology 2003, 19: 333-366. PMID: 14570573, DOI: 10.1146/annurev.cellbio.19.110701.161425.Peer-Reviewed Original ResearchConceptsTransport proteinsMembrane trafficking processesIon transport proteinsIon transport activityProtein traffickingCellular machineryTrafficking processesPolarized cellsPlasma membraneEndocytic retrievalSubcellular distributionPhysiological functionsTransport activityCell surfaceBiochemical natureCell membraneIntracellular populationProteinMembraneTraffickingMachineryRegulationCellsDomain
2001
Ion pump sorting in polarized renal epithelial cells
Caplan M. Ion pump sorting in polarized renal epithelial cells. Kidney International 2001, 60: 427-430. PMID: 11473621, DOI: 10.1046/j.1523-1755.2001.060002427.x.Peer-Reviewed Original ResearchConceptsIon transport proteinsRenal epithelial cellsSubcellular localizationTransport proteinsPolarized renal epithelial cellsEpithelial cellsProtein subcellular localizationBasolateral domainMultiple complex signalsPlasma membranePolarized distributionIon pumpsProteinCellsMembraneLocalizationDomainIon Pumps in Polarized Cells: Sorting and Regulation of the Na+,K+- and H+,K+-ATPases*
Dunbar L, Caplan M. Ion Pumps in Polarized Cells: Sorting and Regulation of the Na+,K+- and H+,K+-ATPases*. Journal Of Biological Chemistry 2001, 276: 29617-29620. PMID: 11404365, DOI: 10.1074/jbc.r100023200.Peer-Reviewed Original ResearchConceptsP-type familyIon transport proteinsDistinct regulatory pathwaysSubcellular localizationPolarized cellsRelated membersRegulatory pathwaysTransport proteinsMolecular signalsATPasesCellular mechanismsIon pumpsEnzymatic activityEpithelial cellsProteinComplex arrayCatalytic capacityPhysiologic functionIntramolecular interactionsCellsHomologyTraffickingATPasePathwayRegulation
1998
Identification of Sorting Determinants in the C-terminal Cytoplasmic Tails of the γ-Aminobutyric Acid Transporters GAT-2 and GAT-3*
Muth T, Ahn J, Caplan M. Identification of Sorting Determinants in the C-terminal Cytoplasmic Tails of the γ-Aminobutyric Acid Transporters GAT-2 and GAT-3*. Journal Of Biological Chemistry 1998, 273: 25616-25627. PMID: 9748227, DOI: 10.1074/jbc.273.40.25616.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBiological TransportCarrier ProteinsCells, CulturedCloning, MolecularDogsFluorescent Antibody TechniqueGABA Plasma Membrane Transport ProteinsGamma-Aminobutyric AcidGenes, mycIon ChannelsKidneyMembrane ProteinsMembrane Transport ProteinsMicroscopy, ConfocalMolecular Sequence DataRecombinant Fusion ProteinsSequence DeletionConceptsC-terminal cytoplasmic tailIon transport proteinsMadin-Darby canine kidney cellsCytoplasmic tailMembrane proteinsC-terminusCanine kidney cellsTransporter familyAmino acidsBasolateral distributionTransport proteinsGAT-2Polytopic membrane proteinsProtein-based signalsProtein-protein interactionsTerminal cytoplasmic tailC-terminal sequencesKidney cellsClass of polypeptidesEpithelial cellsApical sortingPDZ domainChimeric transportersPolarized sortingSorting determinant
1997
Sorting and trafficking of ion transport proteins in polarized epithelial cells
Muth T, Dunbar L, Cortois-Coutry N, Roush D, Caplan M. Sorting and trafficking of ion transport proteins in polarized epithelial cells. Current Opinion In Nephrology & Hypertension 1997, 6: 455-459. PMID: 9327204, DOI: 10.1097/00041552-199709000-00008.Peer-Reviewed Original Research
1996
Immunolocalization of ion transport proteins in human autosomal dominant polycystic kidney epithelial cells.
Brill SR, Ross KE, Davidow CJ, Ye M, Grantham JJ, Caplan MJ. Immunolocalization of ion transport proteins in human autosomal dominant polycystic kidney epithelial cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 10206-10211. PMID: 8816777, PMCID: PMC38362, DOI: 10.1073/pnas.93.19.10206.Peer-Reviewed Original ResearchConceptsCyst epithelial cellsEpithelial cellsCystic fibrosis transmembrane conductance regulatorIon transport proteinsFibrosis transmembrane conductance regulatorK-ATPaseApical cystic fibrosis transmembrane conductance regulatorTransmembrane conductance regulatorKidney epithelial cellsK-ATPase proteinRenal epithelial cellsCFTR proteinTransport proteinsConductance regulatorIntracellular structuresFluid-filled cystsApical membraneForskolin treatmentBasolateral surfacePermeable filter supportsApical surfaceProteinCl- channelsApical labelingAutosomal dominant polycystic kidney disease
1993
Sorting of ion transport proteins in polarized cells
Gottardi C, Pietrini G, Roush D, Caplan M. Sorting of ion transport proteins in polarized cells. Journal Of Cell Science. Supplement 1993, 1993: 13-20. PMID: 8144688, DOI: 10.1242/jcs.1993.supplement_17.3.Peer-Reviewed Original ResearchConceptsIon transport proteinsTransport proteinsSorting signalsSubunit polypeptidesMultiple sorting signalsEpithelial cellsCell typesPlasma membrane domainsPolarized epithelial cellsClass of proteinsEpithelial sortingNon-epithelial cell typesCellular machineryMembrane domainsFunctional homologyPlasmalemmal domainsPlasma membraneRelated membersCell surfaceIon pumpsDistinct populationsProteinRelated familiesDelivery pathwayDistinct routes