2019
Investigation of the role of typhoid toxin in acute typhoid fever in a human challenge model
Gibani MM, Jones E, Barton A, Jin C, Meek J, Camara S, Galal U, Heinz E, Rosenberg-Hasson Y, Obermoser G, Jones C, Campbell D, Black C, Thomaides-Brears H, Darlow C, Dold C, Silva-Reyes L, Blackwell L, Lara-Tejero M, Jiao X, Stack G, Blohmke CJ, Hill J, Angus B, Dougan G, Galán J, Pollard AJ. Investigation of the role of typhoid toxin in acute typhoid fever in a human challenge model. Nature Medicine 2019, 25: 1082-1088. PMID: 31270506, PMCID: PMC6892374, DOI: 10.1038/s41591-019-0505-4.Peer-Reviewed Original ResearchConceptsHuman challenge modelTyphoid toxinChallenge modelTyphoid feverAcute typhoid feverDuration of bacteremiaFurther clinical dataS. typhiAcute infectionOral challengeClinical syndromeChronic infectionBacterial carriageClinical dataSevere diseaseTN groupFever symptomsHealthy volunteersDisease pathogenesisTyphoid infectionTyphoid diseaseColony-forming unitsHost-restricted pathogenInfectionSalmonella typhi
2017
Metabolic and fitness determinants for in vitro growth and intestinal colonization of the bacterial pathogen Campylobacter jejuni
Gao B, Vorwerk H, Huber C, Lara-Tejero M, Mohr J, Goodman AL, Eisenreich W, Galán JE, Hofreuter D. Metabolic and fitness determinants for in vitro growth and intestinal colonization of the bacterial pathogen Campylobacter jejuni. PLOS Biology 2017, 15: e2001390. PMID: 28542173, PMCID: PMC5438104, DOI: 10.1371/journal.pbio.2001390.Peer-Reviewed Original ResearchMeSH KeywordsAbsorption, PhysiologicalAmino AcidsAnimalsAnti-Bacterial AgentsBacterial ProteinsCampylobacter InfectionsCampylobacter jejuniCation Transport ProteinsDNA Transposable ElementsDysbiosisGastroenteritisGene DeletionGenetic Association StudiesGenome, BacterialGenomic LibraryMice, Inbred C57BLMicrobial ViabilityModels, BiologicalMutagenesis, InsertionalMutationConceptsCampylobacter jejuniFood-producing animalsInfectious causesFood-borne illnessMost infectionsMouse modelHuman infectionsIntestinal colonizationIntestinal tractInfectionJejuni colonizationMouse intestineNext-generation sequencingIntracellular ion homeostasisC. jejuniFitness determinantsCertain nutrientsPathogenic strainsJejuniMetabolic requirementsComprehensive genome-wide analysisBacterial pathogen Campylobacter jejuniPathogen Campylobacter jejuniAnimalsGenome-wide analysis
2016
A Family of Salmonella Type III Secretion Effector Proteins Selectively Targets the NF-κB Signaling Pathway to Preserve Host Homeostasis
Sun H, Kamanova J, Lara-Tejero M, Galán JE. A Family of Salmonella Type III Secretion Effector Proteins Selectively Targets the NF-κB Signaling Pathway to Preserve Host Homeostasis. PLOS Pathogens 2016, 12: e1005484. PMID: 26933955, PMCID: PMC4775039, DOI: 10.1371/journal.ppat.1005484.Peer-Reviewed Original ResearchConceptsNF-κBHost homeostasisNF-κB Signaling PathwayHost innate immune responsePro-inflammatory cytokinesInnate immune responseType III secretion effector proteinsHost tissue damageIntestinal inflammationSalmonella typhimurium strainsImmune responseAnimal modelsInflammationRelB transcription factorPathogen replicationMicrobial infectionsTyphimurium strainsEffector proteinsSignaling pathwaysType III secretion systemInfectionBacterial pathogensBacteria Salmonella typhimuriumSignal transduction pathwaysSalmonella typhimurium
2013
Engineering the type III secretion system in non-replicating bacterial minicells for antigen delivery
Carleton HA, Lara-Tejero M, Liu X, Galán JE. Engineering the type III secretion system in non-replicating bacterial minicells for antigen delivery. Nature Communications 2013, 4: 1590. PMID: 23481398, PMCID: PMC3693737, DOI: 10.1038/ncomms2594.Peer-Reviewed Original ResearchConceptsBacterial minicellsPresentation pathwayVaccine developmentClass IAntigen-specific CD8Antigen delivery platformSalmonella typhimurium type III secretion systemDelivery platformType III secretion systemCellular immunotherapyT cellsAntigen deliveryImmune responseHeterologous antigensProtein antigensSecretion systemCertain populationsAntigenNanoparticlesDeliveryNanomachinesCD8ImmunotherapyPathwayNovel approach
2006
A MyD88-Deficient Mouse Model Reveals a Role for Nramp1 in Campylobacter jejuni Infection
Watson RO, Novik V, Hofreuter D, Lara-Tejero M, Galán JE. A MyD88-Deficient Mouse Model Reveals a Role for Nramp1 in Campylobacter jejuni Infection. Infection And Immunity 2006, 75: 1994-2003. PMID: 17194808, PMCID: PMC1865720, DOI: 10.1128/iai.01216-06.Peer-Reviewed Original ResearchConceptsJejuni infectionAdaptor protein myeloid differentiation factor 88Myeloid differentiation factor 88Most Toll-like receptorsMyD88-deficient miceDifferentiation factor 88Campylobacter jejuni infectionAdult immunocompetent miceToll-like receptorsMajor worldwide causeFactor 88Immunocompetent miceMouse modelMouse susceptibilityEnteric illnessWorldwide causeJejuni colonizationInfectionMiceC. jejuniCampylobacter jejuniPotential roleVirulence genesBacterial pathogensIsogenic derivativesRole of the caspase-1 inflammasome in Salmonella typhimurium pathogenesis
Lara-Tejero M, Sutterwala FS, Ogura Y, Grant EP, Bertin J, Coyle AJ, Flavell RA, Galán J. Role of the caspase-1 inflammasome in Salmonella typhimurium pathogenesis. Journal Of Experimental Medicine 2006, 203: 1407-1412. PMID: 16717117, PMCID: PMC2118315, DOI: 10.1084/jem.20060206.Peer-Reviewed Original ResearchCritical Role for NALP3/CIAS1/Cryopyrin in Innate and Adaptive Immunity through Its Regulation of Caspase-1
Sutterwala FS, Ogura Y, Szczepanik M, Lara-Tejero M, Lichtenberger GS, Grant EP, Bertin J, Coyle AJ, Galán JE, Askenase PW, Flavell RA. Critical Role for NALP3/CIAS1/Cryopyrin in Innate and Adaptive Immunity through Its Regulation of Caspase-1. Immunity 2006, 24: 317-327. PMID: 16546100, DOI: 10.1016/j.immuni.2006.02.004.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsApoptosis Regulatory ProteinsAutoimmune DiseasesCARD Signaling Adaptor ProteinsCarrier ProteinsCaspase 1Cytoskeletal ProteinsEnzyme ActivationImmunity, InnateInterleukin-1LipopolysaccharidesMacrophagesMiceMice, Inbred C57BLNLR Family, Pyrin Domain-Containing 3 ProteinRNA, MessengerSalmonella typhimuriumShock, SepticToll-Like ReceptorsConceptsIL-1betaImpaired contact hypersensitivity responseCaspase-1NALP3-deficient miceContact hypersensitivity responseFamilial cold autoinflammatory syndromeCytokines IL-1alphaCaspase-1 functionMuckle-Wells syndromeCaspase-1 activationLipopolysaccharide-stimulated macrophagesContact hypersensitivityHypersensitivity responseIL-18Articular syndromeAutoinflammatory disordersAutoinflammatory syndromesIL-1alphaAdaptive immunityAdaptor molecule ASCCaspase-1 activation pathwayNALP3SyndromeActivation pathwayMice