2017
Visualization and characterization of individual type III protein secretion machines in live bacteria
Zhang Y, Lara-Tejero M, Bewersdorf J, Galán JE. Visualization and characterization of individual type III protein secretion machines in live bacteria. Proceedings Of The National Academy Of Sciences Of The United States Of America 2017, 114: 6098-6103. PMID: 28533372, PMCID: PMC5468683, DOI: 10.1073/pnas.1705823114.Peer-Reviewed Original ResearchMeSH KeywordsBacteriaBacterial ProteinsBacterial Secretion SystemsCluster AnalysisModels, MolecularProtein TransportSalmonella typhimuriumSingle Molecule ImagingType III Secretion SystemsConceptsProtein secretion machinesSecretion machineType III secretion machinesLive bacteriaEukaryotic cellsEffector proteinsMolecular machinesSubcellular distributionSuperresolution microscopyBacteriaMajor insightsResolution gapDetailed viewAssemblyMutantsProteinNanomachinesCharacterizationCellsSpatial distributionIsolationIn Situ Molecular Architecture of the Salmonella Type III Secretion Machine
Hu B, Lara-Tejero M, Kong Q, Galán JE, Liu J. In Situ Molecular Architecture of the Salmonella Type III Secretion Machine. Cell 2017, 168: 1065-1074.e10. PMID: 28283062, PMCID: PMC5393631, DOI: 10.1016/j.cell.2017.02.022.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsBacterial Secretion SystemsCryoelectron MicroscopyProtein TransportSalmonella typhimuriumVirulenceConceptsType III secretion machinesSecretion machineNeedle complexType III protein secretion systemCytoplasmic sorting platformProtein secretion systemMulti-protein machinesProtein secretion machinesTarget eukaryotic cellsCryo-electron tomographyDifferent deletion mutantsSub-tomogram averagingSignificant conformational changesMolecular architectureExport apparatusEukaryotic cellsSecretion systemDeletion mutantsSorting platformConformational changesSitu structureMajor insightsMolecular modelingStructural componentsAssembly
2014
Bacterial Type III Secretion Systems: Specialized Nanomachines for Protein Delivery into Target Cells
Galán JE, Lara-Tejero M, Marlovits TC, Wagner S. Bacterial Type III Secretion Systems: Specialized Nanomachines for Protein Delivery into Target Cells. Annual Review Of Microbiology 2014, 68: 1-24. PMID: 25002086, PMCID: PMC4388319, DOI: 10.1146/annurev-micro-092412-155725.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacteriaBacterial InfectionsBacterial ProteinsBacterial Secretion SystemsHumansProtein TransportConceptsEukaryotic cellsEffector proteinsBacterial envelopeType III secretion systemTarget eukaryotic cellsComplex nanomachinesNovel antimicrobial strategiesPathogen's benefitCellular functionsSecretion systemNeedle complexBacterial pathogenesisCytoplasmic componentsSpecialized nanomachinesSpecific substratesProteinPlantsCentral roleProtein deliveryAntimicrobial strategiesBacteriaNanomachinesTarget cellsCentral componentCellsNMR Model of PrgI–SipD Interaction and Its Implications in the Needle-Tip Assembly of the Salmonella Type III Secretion System
Rathinavelan T, Lara-Tejero M, Lefebre M, Chatterjee S, McShan AC, Guo DC, Tang C, Galan JE, De Guzman RN. NMR Model of PrgI–SipD Interaction and Its Implications in the Needle-Tip Assembly of the Salmonella Type III Secretion System. Journal Of Molecular Biology 2014, 426: 2958-2969. PMID: 24951833, PMCID: PMC4108505, DOI: 10.1016/j.jmb.2014.06.009.Peer-Reviewed Original ResearchConceptsType III secretion systemTip proteinSecretion systemRecent atomic structuresNeedle tip complexN-terminal αSalmonella type III secretion systemDomain bindsHelical hairpinVirulence proteinsFusion proteinHuman cellsSteric clashesSipDProteinPathogenic bacteriaNMR modelsRecent NMRPrgIPrgI.Needle apparatusPRE methodSpecific regionsStructural componentsAssembly
2013
Engineering the type III secretion system in non-replicating bacterial minicells for antigen delivery
Carleton HA, Lara-Tejero M, Liu X, Galán JE. Engineering the type III secretion system in non-replicating bacterial minicells for antigen delivery. Nature Communications 2013, 4: 1590. PMID: 23481398, PMCID: PMC3693737, DOI: 10.1038/ncomms2594.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigensBacterial ProteinsBacterial Secretion SystemsCD8-Positive T-LymphocytesGenetic EngineeringMiceMice, Inbred C57BLOvalbuminSalmonella typhimuriumConceptsBacterial minicellsPresentation pathwayVaccine developmentClass IAntigen-specific CD8Antigen delivery platformSalmonella typhimurium type III secretion systemDelivery platformType III secretion systemCellular immunotherapyT cellsAntigen deliveryImmune responseHeterologous antigensProtein antigensSecretion systemCertain populationsAntigenNanoparticlesDeliveryNanomachinesCD8ImmunotherapyPathwayNovel approach
2011
A Sorting Platform Determines the Order of Protein Secretion in Bacterial Type III Systems
Lara-Tejero M, Kato J, Wagner S, Liu X, Galán JE. A Sorting Platform Determines the Order of Protein Secretion in Bacterial Type III Systems. Science 2011, 331: 1188-1191. PMID: 21292939, PMCID: PMC3859126, DOI: 10.1126/science.1201476.Peer-Reviewed Original ResearchConceptsSecretion systemSorting platformType III protein secretion systemBacterial type III protein secretion systemsType III protein secretionProtein secretionType III secretion systemCytoplasmic sorting platformProtein secretion systemType III systemSalmonella enterica serovar TyphimuriumNovel antimicrobial strategiesEukaryotic cellsEffector proteinsEnterica serovar TyphimuriumCellular processesSerovar TyphimuriumImportant pathogenProteinAntimicrobial strategiesChaperonesSecretionPathogensMechanismSubstrate