2017
Visualization and characterization of individual type III protein secretion machines in live bacteria
Zhang Y, Lara-Tejero M, Bewersdorf J, Galán JE. Visualization and characterization of individual type III protein secretion machines in live bacteria. Proceedings Of The National Academy Of Sciences Of The United States Of America 2017, 114: 6098-6103. PMID: 28533372, PMCID: PMC5468683, DOI: 10.1073/pnas.1705823114.Peer-Reviewed Original ResearchConceptsProtein secretion machinesSecretion machineType III secretion machinesLive bacteriaEukaryotic cellsEffector proteinsMolecular machinesSubcellular distributionSuperresolution microscopyBacteriaMajor insightsResolution gapDetailed viewAssemblyMutantsProteinNanomachinesCharacterizationCellsSpatial distributionIsolationIn Situ Molecular Architecture of the Salmonella Type III Secretion Machine
Hu B, Lara-Tejero M, Kong Q, Galán JE, Liu J. In Situ Molecular Architecture of the Salmonella Type III Secretion Machine. Cell 2017, 168: 1065-1074.e10. PMID: 28283062, PMCID: PMC5393631, DOI: 10.1016/j.cell.2017.02.022.Peer-Reviewed Original ResearchConceptsType III secretion machinesSecretion machineNeedle complexType III protein secretion systemCytoplasmic sorting platformProtein secretion systemMulti-protein machinesProtein secretion machinesTarget eukaryotic cellsCryo-electron tomographyDifferent deletion mutantsSub-tomogram averagingSignificant conformational changesMolecular architectureExport apparatusEukaryotic cellsSecretion systemDeletion mutantsSorting platformConformational changesSitu structureMajor insightsMolecular modelingStructural componentsAssembly
2014
Bacterial Type III Secretion Systems: Specialized Nanomachines for Protein Delivery into Target Cells
Galán JE, Lara-Tejero M, Marlovits TC, Wagner S. Bacterial Type III Secretion Systems: Specialized Nanomachines for Protein Delivery into Target Cells. Annual Review Of Microbiology 2014, 68: 1-24. PMID: 25002086, PMCID: PMC4388319, DOI: 10.1146/annurev-micro-092412-155725.Peer-Reviewed Original ResearchConceptsEukaryotic cellsEffector proteinsBacterial envelopeType III secretion systemTarget eukaryotic cellsComplex nanomachinesNovel antimicrobial strategiesPathogen's benefitCellular functionsSecretion systemNeedle complexBacterial pathogenesisCytoplasmic componentsSpecialized nanomachinesSpecific substratesProteinPlantsCentral roleProtein deliveryAntimicrobial strategiesBacteriaNanomachinesTarget cellsCentral componentCells
2011
A Sorting Platform Determines the Order of Protein Secretion in Bacterial Type III Systems
Lara-Tejero M, Kato J, Wagner S, Liu X, Galán JE. A Sorting Platform Determines the Order of Protein Secretion in Bacterial Type III Systems. Science 2011, 331: 1188-1191. PMID: 21292939, PMCID: PMC3859126, DOI: 10.1126/science.1201476.Peer-Reviewed Original ResearchConceptsSecretion systemSorting platformType III protein secretion systemBacterial type III protein secretion systemsType III protein secretionProtein secretionType III secretion systemCytoplasmic sorting platformProtein secretion systemType III systemSalmonella enterica serovar TyphimuriumNovel antimicrobial strategiesEukaryotic cellsEffector proteinsEnterica serovar TyphimuriumCellular processesSerovar TyphimuriumImportant pathogenProteinAntimicrobial strategiesChaperonesSecretionPathogensMechanismSubstrate
2010
Organization and coordinated assembly of the type III secretion export apparatus
Wagner S, Königsmaier L, Lara-Tejero M, Lefebre M, Marlovits TC, Galán JE. Organization and coordinated assembly of the type III secretion export apparatus. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 17745-17750. PMID: 20876096, PMCID: PMC2955140, DOI: 10.1073/pnas.1008053107.Peer-Reviewed Original ResearchConceptsExport apparatusNeedle complexType III protein secretion systemType III secretion machinesBacterial effector proteinsProtein secretion systemType III secretion export apparatusCryo-electron microscopyBacterial nanomachinesSecretion machineSymbiotic bacteriaEukaryotic cellsEffector proteinsSecretion systemMembrane proteinsApparatus componentsSubstantial coordinationBase assemblyProteinAssemblyEssential componentInsectsOrganellesComplexesBiology