Direct Detection and Magnetic Isolation of Chlamydia trachomatis Major Outer Membrane Protein-Specific CD8+ CTLs with HLA Class I Tetramers
Kim S, Devine L, Angevine M, DeMars R, Kavathas P. Direct Detection and Magnetic Isolation of Chlamydia trachomatis Major Outer Membrane Protein-Specific CD8+ CTLs with HLA Class I Tetramers. The Journal Of Immunology 2000, 165: 7285-7292. PMID: 11120863, DOI: 10.4049/jimmunol.165.12.7285.Peer-Reviewed Original ResearchMeSH KeywordsBacterial Outer Membrane ProteinsCells, CulturedChlamydia trachomatisCytotoxicity Tests, ImmunologicEpitopes, T-LymphocyteFemaleHematopoietic Stem CellsHLA-A2 AntigenHLA-B AntigensHumansImmunomagnetic SeparationLymphocyte CountLymphogranuloma VenereumMalePorinsProtein BindingT-Lymphocytes, CytotoxicConceptsHLA class ITetramer-binding T cellsCTL responsesT cellsPeripheral bloodClass IPeptide-specific T cellsHLA-A2 tetramersPeptide-pulsed targetsGenital tract infectionElicit CTL responsesMajor outer membrane proteinTetramer analysisTract infectionsPeptide stimulationImmunization trialsCytolytic activityChlamydia trachomatisUninfected individualsVivo inductionInfected individualsEpithelial cellsBloodBulk culturesStimulation