1989
A growth hormone-vesicular stomatitis virus G hybrid protein is rapidly degraded in lysosomes following transport to the cell surface.
Rizzolo L. A growth hormone-vesicular stomatitis virus G hybrid protein is rapidly degraded in lysosomes following transport to the cell surface. European Journal Of Cell Biology 1989, 49: 92-8. PMID: 2547615.Peer-Reviewed Original Research
1986
Secretion of endogenous and exogenous proteins from polarized MDCK cell monolayers.
Gottlieb T, Beaudry G, Rizzolo L, Colman A, Rindler M, Adesnik M, Sabatini D. Secretion of endogenous and exogenous proteins from polarized MDCK cell monolayers. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 2100-2104. PMID: 3083413, PMCID: PMC323238, DOI: 10.1073/pnas.83.7.2100.Peer-Reviewed Original ResearchConceptsMDCK cellsCellular endomembrane systemEpithelial cell polarityMembrane-associated precursorEndogenous productsMature secretory productCell polarityPolarized MDCK cell monolayersEndomembrane systemAlpha 2u-globulinCorresponding genesSecretory pathwayMDCK cell monolayersRegulated pathwaySecretory productsImmunoglobulin kappa chainConstitutive secretionExogenous proteinsProteolytic cleavageSorting mechanismBasolateral surfaceApical surfaceProteinVectorial secretionCell of originSorting and endocytosis of viral glycoproteins in transfected polarized epithelial cells.
Gottlieb T, Gonzalez A, Rizzolo L, Rindler M, Adesnik M, Sabatini D. Sorting and endocytosis of viral glycoproteins in transfected polarized epithelial cells. Journal Of Cell Biology 1986, 102: 1242-1255. PMID: 3007530, PMCID: PMC2114186, DOI: 10.1083/jcb.102.4.1242.Peer-Reviewed Original ResearchConceptsG proteinsCell surfaceVesicular stomatitis virusApical plasma membrane domainPlasma membrane domainsPlasma membrane proteinsCell surface domainsApical cell surfaceMadin-Darby canine kidney cellsG protein moleculesViral glycoproteinsLysosome-like vesiclesCanine kidney cellsProtein synthesis inhibitorMembrane domainsMembrane proteinsSurface domainsBasolateral domainEukaryotic expression plasmidTransfection experimentsExpression plasmidProteinPresence of chloroquineApical surfaceProtein molecules
1985
Biosynthesis and intracellular sorting of growth hormone-viral envelope glycoprotein hybrids.
Rizzolo L, Finidori J, Gonzalez A, Arpin M, Ivanov I, Adesnik M, Sabatini D. Biosynthesis and intracellular sorting of growth hormone-viral envelope glycoprotein hybrids. Journal Of Cell Biology 1985, 101: 1351-1362. PMID: 2995406, PMCID: PMC2113904, DOI: 10.1083/jcb.101.4.1351.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCell LineChlorocebus aethiopsDogsEndoplasmic ReticulumFibroblastsGenes, SyntheticGenetic VectorsGlycoproteinsGrowth HormoneHemagglutinins, ViralIntracellular MembranesKidneyMembrane ProteinsOrthomyxoviridaeProtein Processing, Post-TranslationalRecombinant ProteinsTransfectionVesicular stomatitis Indiana virusViral Envelope ProteinsConceptsCarboxy-terminal portionGolgi apparatusMembrane proteinsChimeric geneTranscription-translation experimentsPlasma membrane proteinsEndoplasmic reticulum membraneMembrane protein segmentsVesicular stomatitis virus envelope glycoproteinER membraneHybrid proteinIntracellular sortingCytoplasmic portionJuxtanuclear regionTransmembrane portionMembrane polypeptidesCarboxy terminusChimeric proteinHybrid polypeptideInsertion signalProtein segmentsSecretory polypeptidesCytoplasmic vesiclesReticulum membraneER cisternae