2020
RPE Polarity and Barrier Function
Rizzolo L. RPE Polarity and Barrier Function. 2020, 19-45. DOI: 10.1007/978-3-030-28384-1_2.ChaptersCell polarityEpithelial cell polarityPlasma membrane domainsTight junctionsBasolateral plasma membraneRPE polarityCellular processesMembrane domainsPlasma membraneMembrane transportersMembrane transportNeighboring cellsRPE phenotypeSemi-selective barrierProteinBarrier functionJunction proteinsRPE functionParacellular spaceOuter blood-retinal barrierVisual cycleTight junction proteinsTransepithelial electrical resistanceAsymmetric distributionDynamic interplay
1998
Protein-Binding Domains of the Tight Junction Protein, ZO-2, Are Highly Conserved between Avian and Mammalian Species
Collins J, Rizzolo L. Protein-Binding Domains of the Tight Junction Protein, ZO-2, Are Highly Conserved between Avian and Mammalian Species. Biochemical And Biophysical Research Communications 1998, 252: 617-622. PMID: 9837755, DOI: 10.1006/bbrc.1998.9710.Peer-Reviewed Original ResearchConceptsProtein-binding domainsSrc homology 3ZO-2ZO-3Homologous domainsMAGUK protein familyDistinct PDZ domainsProtein binding domainsZO-1Apical junctional complexHomology 3PDZ domainProtein familySH3 domainAcidic domainEvolutionary pressureMammalian speciesJunctional complexesEmbryonic chicken retinaJunction proteinsTight junctionsDifferent functionsProteinTight junction proteinsSequencePolarization of the Na+, K+-ATPase in Epithelia Derived from the Neuroepithelium
Rizzolo L. Polarization of the Na+, K+-ATPase in Epithelia Derived from the Neuroepithelium. International Review Of Cytology 1998, 185: 195-235. PMID: 9750268, DOI: 10.1016/s0074-7696(08)60152-7.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsProper membrane domainDifferent sorting signalsPolarity mechanismsSorting signalsMembrane domainsMembrane proteinsCortical cytoskeletonCertain environmental stimuliFascinating groupMaintenance signalsApical membraneEnvironmental stimuliIon pumpsProteinBasolateral membraneATPaseYields insightsNeuroepitheliumMembraneCellsCytoskeletonEpitheliumRetinal pigment epitheliumSignalsPigment epithelium
1995
The distribution of Na+,K+-atpase and 5a11 antigen in apical microvilli of the retinal pigment epithelium is unrelated to α-spectrin
Rizzolo L, Zhou S. The distribution of Na+,K+-atpase and 5a11 antigen in apical microvilli of the retinal pigment epithelium is unrelated to α-spectrin. Journal Of Cell Science 1995, 108: 3623-3633. PMID: 8586673, DOI: 10.1242/jcs.108.11.3623.Peer-Reviewed Original ResearchConceptsAlpha-spectrinPlasma membranePlasma membrane proteinsDevelopment of polarityLateral plasma membraneBasal plasma membraneMembrane proteinsCortical cytoskeletonMacromolecular complexesBeta spectrinAnkyrinChick embryogenesisApical poleCytoskeletonEmbryonic day 6RPE microvilliLateral membranesReceptor proteinMost epitheliaSpectrin distributionΑ-spectrinApical membraneIon pumpsApical microvilliProtein
1987
Nonpolarized secretion of truncated forms of the influenza hemagglutinin and the vesicular stomatitus virus G protein from MDCK cells.
Gonzalez A, Rizzolo L, Rindler M, Adesnik M, Sabatini D, Gottlieb T. Nonpolarized secretion of truncated forms of the influenza hemagglutinin and the vesicular stomatitus virus G protein from MDCK cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1987, 84: 3738-3742. PMID: 3035552, PMCID: PMC304951, DOI: 10.1073/pnas.84.11.3738.Peer-Reviewed Original ResearchConceptsApical plasma membrane domainMDCK cellsPlasma membrane domainsPolarity of secretionVirus G proteinViral glycoproteinsVesicular stomatitus virusMembrane anchoringCorresponding genesMembrane domainsCytoplasmic segmentNonpolarized fashionG proteinsEndogenous glycoproteinsApical surfaceProteinEpithelial cellsGlycoprotein GG glycoproteinGlycoproteinConfluent monolayersCellsInfluenza hemagglutininCDNAGenes
1986
Secretion of endogenous and exogenous proteins from polarized MDCK cell monolayers.
Gottlieb T, Beaudry G, Rizzolo L, Colman A, Rindler M, Adesnik M, Sabatini D. Secretion of endogenous and exogenous proteins from polarized MDCK cell monolayers. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 2100-2104. PMID: 3083413, PMCID: PMC323238, DOI: 10.1073/pnas.83.7.2100.Peer-Reviewed Original ResearchConceptsMDCK cellsCellular endomembrane systemEpithelial cell polarityMembrane-associated precursorEndogenous productsMature secretory productCell polarityPolarized MDCK cell monolayersEndomembrane systemAlpha 2u-globulinCorresponding genesSecretory pathwayMDCK cell monolayersRegulated pathwaySecretory productsImmunoglobulin kappa chainConstitutive secretionExogenous proteinsProteolytic cleavageSorting mechanismBasolateral surfaceApical surfaceProteinVectorial secretionCell of originSorting and endocytosis of viral glycoproteins in transfected polarized epithelial cells.
Gottlieb T, Gonzalez A, Rizzolo L, Rindler M, Adesnik M, Sabatini D. Sorting and endocytosis of viral glycoproteins in transfected polarized epithelial cells. Journal Of Cell Biology 1986, 102: 1242-1255. PMID: 3007530, PMCID: PMC2114186, DOI: 10.1083/jcb.102.4.1242.Peer-Reviewed Original ResearchConceptsG proteinsCell surfaceVesicular stomatitis virusApical plasma membrane domainPlasma membrane domainsPlasma membrane proteinsCell surface domainsApical cell surfaceMadin-Darby canine kidney cellsG protein moleculesViral glycoproteinsLysosome-like vesiclesCanine kidney cellsProtein synthesis inhibitorMembrane domainsMembrane proteinsSurface domainsBasolateral domainEukaryotic expression plasmidTransfection experimentsExpression plasmidProteinPresence of chloroquineApical surfaceProtein molecules