2021
Targeted RNAseq assay incorporating unique molecular identifiers for improved quantification of gene expression signatures and transcribed mutation fraction in fixed tumor samples
Fu C, Marczyk M, Samuels M, Trevarton AJ, Qu J, Lau R, Du L, Pappas T, Sinn BV, Gould RE, Pusztai L, Hatzis C, Symmans WF. Targeted RNAseq assay incorporating unique molecular identifiers for improved quantification of gene expression signatures and transcribed mutation fraction in fixed tumor samples. BMC Cancer 2021, 21: 114. PMID: 33541297, PMCID: PMC7860187, DOI: 10.1186/s12885-021-07814-8.Peer-Reviewed Original ResearchConceptsPolymerase chain reactionParaffin-embedded tumor tissue samplesConcordance correlation coefficientFresh frozenFFPE samplesPrimary breast cancerMulti-gene signatureTumor tissue samplesActivating point mutationMutant allele fractionReverse transcriptionKey breast cancer genesGene expression signaturesBreast cancer genesPIK3CA mutationsBackgroundOur objectiveBreast cancerWhole transcriptome RNAseqTumor samplesLin's concordance correlation coefficientHormone receptorsFF samplesTissue samplesExpression signaturesChain reaction
2012
Agreement in Risk Prediction Between the 21‐Gene Recurrence Score Assay (Oncotype DX®) and the PAM50 Breast Cancer Intrinsic Classifier™ in Early‐Stage Estrogen Receptor–Positive Breast Cancer
Kelly CM, Bernard PS, Krishnamurthy S, Wang B, Ebbert MT, Bastien RR, Boucher KM, Young E, Iwamoto T, Pusztai L. Agreement in Risk Prediction Between the 21‐Gene Recurrence Score Assay (Oncotype DX®) and the PAM50 Breast Cancer Intrinsic Classifier™ in Early‐Stage Estrogen Receptor–Positive Breast Cancer. The Oncologist 2012, 17: 492-498. PMID: 22418568, PMCID: PMC3336833, DOI: 10.1634/theoncologist.2012-0007.Peer-Reviewed Original ResearchConceptsBreast cancerEstrogen receptorEarly-stage estrogen receptor-positive breast cancerRisk assignmentHuman epidermal growth factor receptor 2 (HER2) expressionEpidermal growth factor receptor 2 expressionEstrogen receptor-positive breast cancerReceptor-positive breast cancerIntermediate RS groupLuminal B cancersReceptor 2 expressionLow-risk categoryQuantitative polymerase chain reactionB cancersMore patientsPolymerase chain reactionIntermediate RSLower riskStage IRS groupCancerPAM50Risk categoriesRisk predictionChain reaction
2006
Molecular Classification of Breast Cancer: Limitations and Potential
Pusztai L, Mazouni C, Anderson K, Wu Y, Symmans WF. Molecular Classification of Breast Cancer: Limitations and Potential. The Oncologist 2006, 11: 868-877. PMID: 16951390, DOI: 10.1634/theoncologist.11-8-868.Peer-Reviewed Original ResearchConceptsBreast cancerMolecular classificationNovel risk stratification methodsEstrogen receptor immunohistochemistryBreast cancer patientsRisk stratification methodsRoutine diagnostic workupTranscription-polymerase chain reactionNew molecular classificationReceptor immunohistochemistryCancer patientsDiagnostic workupPolymerase chain reactionBest therapyMolecular subsetsPredictive valueClinical researchCancerDiagnostic testsPredictive testChain reactionMedical decisions
2004
Technology Insight: emerging techniques to predict response to preoperative chemotherapy in breast cancer
Pusztai L, Gianni L. Technology Insight: emerging techniques to predict response to preoperative chemotherapy in breast cancer. Nature Reviews Clinical Oncology 2004, 1: 44-50. PMID: 16264799, DOI: 10.1038/ncponc0025.Peer-Reviewed Original ResearchConceptsBreast cancerOptimal chemotherapy regimenHistopathologic predictorsChemotherapy regimenChemotherapy regimensPreoperative chemotherapyPolymerase chain reactionPotential diagnostic toolMultiplex polymerase chain reactionClinical useGene expressionChain reactionDiagnostic toolCancerMeaningful responsesRegimenRegimensChemotherapyPatientsPrognosis