2021
Admission Lymphopenia is Associated With Discharge Disposition in Blunt Chest Wall Trauma Patients
Koch K, Troester A, Chevuru P, Campbell B, Galet C, McGonagill P. Admission Lymphopenia is Associated With Discharge Disposition in Blunt Chest Wall Trauma Patients. Journal Of Surgical Research 2021, 270: 293-299. PMID: 34717263, PMCID: PMC8712416, DOI: 10.1016/j.jss.2021.09.013.Peer-Reviewed Original ResearchConceptsBlunt chest wall traumaAbsolute lymphocyte countChest Abbreviated Injury ScoreAdmission lymphopeniaBlunt chest wall trauma patientsLevel I trauma centerInstitution's trauma registryAbbreviated Injury ScoreI trauma centerInjury Severity ScoreChest wall traumaImportant prognostic informationBowel perforationHospital deathHospital stayAdult patientsCritical illnessDischarge dispositionLymphocyte countTrauma patientsClinical outcomesInjury characteristicsTrauma centerTrauma registryImmune suppressionAP-2alpha-mediated activation of E2F and EZH2 drives melanoma metastasis
White J, Thompson D, Koch K, Kiriazov B, Beck A, van der Heide D, Grimm B, Kulak M, Weigel R. AP-2alpha-mediated activation of E2F and EZH2 drives melanoma metastasis. Cancer Research 2021, 81: canres.0772.2021. PMID: 34210752, PMCID: PMC8416798, DOI: 10.1158/0008-5472.can-21-0772.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Protein Complex 2Adaptor Protein Complex alpha SubunitsAnimalsBase SequenceBenzamidesBiomarkersBiphenyl CompoundsCell Line, TumorE2F Transcription FactorsEnhancer of Zeste Homolog 2 ProteinEpigenesis, GeneticHumansMelanocytesMelanomaMiceMice, Inbred NODMice, SCIDMorpholinesNeoplasm MetastasisNeoplasm TransplantationNeoplasms, Second PrimaryPromoter Regions, GeneticPyridonesSingle-Cell AnalysisTranscription Factor AP-2ConceptsAP-2αRepression complexEZH2 inhibitorsAP-2α transcription factorSingle-cell RNA sequencing analysisE2F target genesRNA sequencing analysisSpecific EZH2 inhibitorsAP-2α expressionActivation of E2FAnchorage-independent colony formationMelanocyte stem cellsTumor suppressor activityChromatin marksNucleosome remodelingBioID screenE2F pathwayTranscriptional activationTranscription factorsTarget genesPathway genesAP-2alphaSequencing analysisMelanoma metastasesGenesMale sex, ostomy, infection, and intravenous fluids are associated with increased risk of postoperative ileus in elective colorectal surgery
Koch K, Hahn A, Hart A, Kahl A, Charlton M, Kapadia M, Hrabe J, Cromwell J, Hassan I, Gribovskaja-Rupp I. Male sex, ostomy, infection, and intravenous fluids are associated with increased risk of postoperative ileus in elective colorectal surgery. Surgery 2021, 170: 1325-1330. PMID: 34210525, DOI: 10.1016/j.surg.2021.05.035.Peer-Reviewed Original ResearchConceptsElective colorectal surgeryAcute kidney injuryEnhanced recovery protocolPostoperative ileusIntravenous fluidsRenal functionMale sexColorectal resectionKidney injuryColorectal surgeryIleorectal anastomosisHigh riskTertiary academic medical centerRecovery protocolElective colorectal resectionIntravenous fluid administrationIntravenous fluid volumeNasogastric tube insertionAcademic medical centerIntestinal reconstructionNasogastric decompressionRenal damageSecondary outcomesColorectal anastomosisPostoperative infectionAP-2α Regulates S-Phase and Is a Marker for Sensitivity to PI3K Inhibitor Buparlisib in Colon Cancer
Beck AC, Cho E, White JR, Paemka L, Li T, Gu VW, Thompson DT, Koch KE, Franke C, Gosse M, Wu VT, Landers SR, Pamatmat AJ, Kulak MV, Weigel RJ. AP-2α Regulates S-Phase and Is a Marker for Sensitivity to PI3K Inhibitor Buparlisib in Colon Cancer. Molecular Cancer Research 2021, 19: 1156-1167. PMID: 33753551, PMCID: PMC8254761, DOI: 10.1158/1541-7786.mcr-20-0867.Peer-Reviewed Original ResearchMeSH KeywordsAminopyridinesAnimalsBiomarkers, TumorCell Line, TumorCell SurvivalColonic NeoplasmsGene Expression ProfilingGene Expression Regulation, NeoplasticGene Knockout TechniquesHCT116 CellsHumansMiceMorpholinesPhosphoinositide-3 Kinase InhibitorsRNA InterferenceRNA-SeqS PhaseTranscription Factor AP-2Xenograft Model Antitumor AssaysConceptsAP-2αPI3K inhibitorsColon cancer cell linesCell cycleS phasePrimary gene targetsK inhibitorsChromatin immunoprecipitation sequencingCancer cell linesPI3K cascadeProlonged S phaseCell linesActivation of AktShort hairpin RNAPhosphorylation of AktHistone H3Immunoprecipitation sequencingRNA sequencingPI3K inhibitionTarget genesK cascadeGene targetsTumor suppressorHairpin RNAColon cancer
2020
Impact of routine mismatch repair screening on genetic counseling and surgical management in colorectal cancer patients
Koch K, Goffredo P, Hrabe J, Gribovskaja-Rupp I, Snow A, Bellizzi A, Kapadia M. Impact of routine mismatch repair screening on genetic counseling and surgical management in colorectal cancer patients. The American Journal Of Surgery 2020, 222: 408-412. PMID: 33317812, DOI: 10.1016/j.amjsurg.2020.12.017.Peer-Reviewed Original ResearchConceptsExtended resectionGenetic counselingResection extentColorectal cancerLynch syndromeCRC specimensMMR deficiencySingle-institution retrospective reviewMMR-deficient patientsSurgical resection extentColorectal cancer patientsMLH1 promoter hypermethylationMismatch repair deficiencyLynch syndrome diagnosisMMR testingRetrospective reviewSegmental resectionSurgical managementCancer patientsLS patientsPatientsResectionSyndrome diagnosisRepair deficiencyFurther studiesTeaching Patient-Related Communication to Surgical Residents in Brief Training Sessions
Kapadia M, White A, Peters L, Kreiter C, Koch K, Rosenbaum M. Teaching Patient-Related Communication to Surgical Residents in Brief Training Sessions. Journal Of Surgical Education 2020, 77: 1496-1502. PMID: 32534941, DOI: 10.1016/j.jsurg.2020.05.006.Peer-Reviewed Original ResearchConceptsSurgical residency programsSurgical residentsEffective provider-patient communicationResidency programsProvider-patient communicationResidents' communication skillsTime-limited interventionSelf-reported useTertiary careClinical examinationHistory takingAcademic centersEligible residentsResident communicationRating ScalePatient interactionsBrief training sessionIndependent ratersCommunication skillsPostcurriculum scoresTraining sessionsBrief didacticsCommunication trainingScoresOSCE scoresA TFAP2C Gene Signature Is Predictive of Outcome in HER2-Positive Breast Cancer
Wu VT, Kiriazov B, Koch KE, Gu VW, Beck AC, Borcherding N, Li T, Addo P, Wehrspan ZJ, Zhang W, Braun TA, Brown BJ, Band V, Band H, Kulak MV, Weigel RJ. A TFAP2C Gene Signature Is Predictive of Outcome in HER2-Positive Breast Cancer. Molecular Cancer Research 2020, 18: 46-56. PMID: 31619506, PMCID: PMC6942205, DOI: 10.1158/1541-7786.mcr-19-0359.Peer-Reviewed Original ResearchConceptsBreast cancerGene signatureAP-2γHER2-positive breast cancerOutcomes of patientsExpression of estrogenSubset of patientsLuminal breast cancerSubset of HER2AP-2γ transcription factorBreast cancer subtypesPathway analysisPhysiologic effectsClinical relevanceCancer growthHER2Hormone responseCancer subtypesDifferent HER2CancerPatientsRetinoic acid pathwayCancer linesOutcomesCell lines