2023
Megahertz Sampling of Prestin (SLC26a5) Voltage-Sensor Charge Movements in Outer Hair Cell Membranes Reveals Ultrasonic Activity that May Support Electromotility and Cochlear Amplification
Santos-Sacchi J, Bai J, Navaratnam D. Megahertz Sampling of Prestin (SLC26a5) Voltage-Sensor Charge Movements in Outer Hair Cell Membranes Reveals Ultrasonic Activity that May Support Electromotility and Cochlear Amplification. Journal Of Neuroscience 2023, 43: 2460-2468. PMID: 36868859, PMCID: PMC10082455, DOI: 10.1523/jneurosci.2033-22.2023.Peer-Reviewed Original ResearchConceptsConformational switchingMammalian hearingMembrane motor proteinCochlear amplificationMotor protein prestinVoltage-sensor charge movementOuter hair cell electromotilityHair cell electromotilityProtein prestinMotor proteinsConformation switchingCharge movementPrestinPrestin activityCell membraneOHC electromotilityHair cell membraneElectromotilityTransmembrane voltageOrgan of CortiAmplificationMammalsMembrane capacitanceProtein
2022
Outer hair cell function is normal in βV spectrin knockout mice
Stankewich MC, Bai JP, Stabach PR, Khan S, Tan WJT, Surguchev A, Song L, Morrow JS, Santos-Sacchi J, Navaratnam DS. Outer hair cell function is normal in βV spectrin knockout mice. Hearing Research 2022, 423: 108564. PMID: 35864018, DOI: 10.1016/j.heares.2022.108564.Peer-Reviewed Original ResearchConceptsOuter hair cellsAuditory brainstem response wavesAuditory thresholdOuter hair cell functionSpiral ganglion neuronsEfferent nerve fibersHair cell functionNumber of afferentsGanglion neuronsNerve fibersKnockout miceNeuronal structuresMiceHair cellsCell functionElectromechanical activityPutative roleType IOngoing investigationExon deletionsSynaptopathyAfferentsData supportResponse wavesNeuronsPhysiology and biophysics of outer hair cells: The cells of Dallos
Santos-Sacchi J, Navaratnam D. Physiology and biophysics of outer hair cells: The cells of Dallos. Hearing Research 2022, 423: 108525. PMID: 35599199, DOI: 10.1016/j.heares.2022.108525.Peer-Reviewed Original Research
2021
Coupling between outer hair cell electromotility and prestin sensor charge depends on voltage operating point
Santos-Sacchi J, Tan WJT. Coupling between outer hair cell electromotility and prestin sensor charge depends on voltage operating point. Hearing Research 2021, 423: 108373. PMID: 34776274, PMCID: PMC9054947, DOI: 10.1016/j.heares.2021.108373.Peer-Reviewed Original ResearchConceptsOperating voltage rangeFrequency responseAC voltage excitationSlow frequency responseVoltage operating pointNonlinear capacitanceOperating voltageOperating pointVoltage excitationVoltage rangeSpecial Issue Outer hair cellJoseph Santos-SacchiVoltageKumar NavaratnamLoadVoltage-sensor charge movementGenes related to SNPs identified by Genome-wide association studies of age-related hearing loss show restriction to specific cell types in the adult mouse cochlea
Xue N, Song L, Song Q, Santos-Sacchi J, Wu H, Navaratnam D. Genes related to SNPs identified by Genome-wide association studies of age-related hearing loss show restriction to specific cell types in the adult mouse cochlea. Hearing Research 2021, 410: 108347. PMID: 34536825, DOI: 10.1016/j.heares.2021.108347.Peer-Reviewed Original ResearchConceptsSingle-cell RNA sequencingApical cell polarityGenome-wide association studiesAdult mouse cochleaSpecific cell typesCell polaritySubtypes of cellsSignificant genetic componentSingle nucleotide polymorphismsVesicle recyclingRNA sequencingAssociation studiesGenesVarying ExpressionCell typesGenetic componentNucleotide polymorphismsSNPsMouse cochleaCell functionGenesetsHair cellsHair cell functionSignificant overlapExpressionState dependent effects on the frequency response of prestin’s real and imaginary components of nonlinear capacitance
Santos-Sacchi J, Navaratnam D, Tan WJT. State dependent effects on the frequency response of prestin’s real and imaginary components of nonlinear capacitance. Scientific Reports 2021, 11: 16149. PMID: 34373481, PMCID: PMC8352928, DOI: 10.1038/s41598-021-95121-4.Peer-Reviewed Original ResearchEfferent feedback controls bilateral auditory spontaneous activity
Wang Y, Sanghvi M, Gribizis A, Zhang Y, Song L, Morley B, Barson DG, Santos-Sacchi J, Navaratnam D, Crair M. Efferent feedback controls bilateral auditory spontaneous activity. Nature Communications 2021, 12: 2449. PMID: 33907194, PMCID: PMC8079389, DOI: 10.1038/s41467-021-22796-8.Peer-Reviewed Original ResearchConceptsSpontaneous activityEfferent modulationEfferent pathwaysMedial olivocochlear systemCentral nervous systemCentral auditory systemInner hair cellsAuditory systemNicotinic acetylcholine receptorsSpontaneous activity patternsOlivocochlear systemHearing onsetEfferent systemChemogenetic experimentsBilateral couplingNervous systemAcetylcholine receptorsCircuit formationEfferent feedbackFiring patternsHair cellsΑ9/Auditory sensitivityBilateral correlationActivity patterns
2019
Outer hair cell electromotility is low-pass filtered relative to the molecular conformational changes that produce nonlinear capacitance
Santos-Sacchi J, Iwasa KH, Tan W. Outer hair cell electromotility is low-pass filtered relative to the molecular conformational changes that produce nonlinear capacitance. The Journal Of General Physiology 2019, 151: 1369-1385. PMID: 31676485, PMCID: PMC6888751, DOI: 10.1085/jgp.201812280.Peer-Reviewed Original ResearchThe speed limit of outer hair cell electromechanical activity
Santos-Sacchi J. The speed limit of outer hair cell electromechanical activity. HNO 2019, 67: 159-164. PMID: 30747242, DOI: 10.1007/s00106-019-0615-9.Peer-Reviewed Original Research
2011
MicroRNA181a plays a key role in hair cell regeneration in the avian auditory epithelium
Frucht CS, Santos-Sacchi J, Navaratnam DS. MicroRNA181a plays a key role in hair cell regeneration in the avian auditory epithelium. Neuroscience Letters 2011, 493: 44-48. PMID: 21316421, PMCID: PMC3065390, DOI: 10.1016/j.neulet.2011.02.017.Peer-Reviewed Original ResearchConceptsHair cell regenerationHair cell deathAuditory epitheliumChicken auditory epitheliumCell deathNon-mammalian vertebratesAvian auditory epitheliumCell regenerationHair cellsBasilar papillaNew hair cellsChicken basilar papillaAvian counterpartsOtotoxic injuryCaspase-3MiRNACellsMiR181aKey roleExplantsProliferationRegenerationBrdU-positive cellsVertebratesMammals
2010
A highly expressing Tet-inducible cell line recapitulates in situ developmental changes in prestin's Boltzmann characteristics and reveals early maturational events
Bian S, Koo BW, Kelleher S, Santos-Sacchi J, Navaratnam DS. A highly expressing Tet-inducible cell line recapitulates in situ developmental changes in prestin's Boltzmann characteristics and reveals early maturational events. American Journal Of Physiology - Cell Physiology 2010, 299: c828-c835. PMID: 20631244, PMCID: PMC3774197, DOI: 10.1152/ajpcell.00182.2010.Peer-Reviewed Original ResearchThe remarkable cochlear amplifier
Ashmore J, Avan P, Brownell W, Dallos P, Dierkes K, Fettiplace R, Grosh K, Hackney C, Hudspeth A, Jülicher F, Lindner B, Martin P, Meaud J, Petit C, Santos-Sacchi J, Sacchi J, Canlon B. The remarkable cochlear amplifier. Hearing Research 2010, 266: 1-17. PMID: 20541061, PMCID: PMC6366996, DOI: 10.1016/j.heares.2010.05.001.Peer-Reviewed Original ResearchPrestin Surface Expression and Activity Are Augmented by Interaction with MAP1S, a Microtubule-associated Protein*
Bai JP, Surguchev A, Ogando Y, Song L, Bian S, Santos-Sacchi J, Navaratnam D. Prestin Surface Expression and Activity Are Augmented by Interaction with MAP1S, a Microtubule-associated Protein*. Journal Of Biological Chemistry 2010, 285: 20834-20843. PMID: 20418376, PMCID: PMC2898336, DOI: 10.1074/jbc.m110.117853.Peer-Reviewed Original Research
2006
Control of Mammalian Cochlear Amplification by Chloride Anions
Santos-Sacchi J, Song L, Zheng J, Nuttall AL. Control of Mammalian Cochlear Amplification by Chloride Anions. Journal Of Neuroscience 2006, 26: 3992-3998. PMID: 16611815, PMCID: PMC6673883, DOI: 10.1523/jneurosci.4548-05.2006.Peer-Reviewed Original Research
1995
Expression in cochlea and retina of myosin VIIa, the gene product defective in Usher syndrome type 1B.
Hasson T, Heintzelman M, Santos-Sacchi J, Corey D, Mooseker M. Expression in cochlea and retina of myosin VIIa, the gene product defective in Usher syndrome type 1B. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 9815-9819. PMID: 7568224, PMCID: PMC40893, DOI: 10.1073/pnas.92.21.9815.Peer-Reviewed Original ResearchMeSH KeywordsAbnormalities, MultipleAmino Acid SequenceAnimalsAntibody SpecificityBlindnessCochleaDeafnessDyneinsFluorescent Antibody TechniqueGuinea PigsHearing Loss, SensorineuralHumansImmunoblottingMolecular Sequence DataMyosin VIIaMyosinsRatsRetinaRetinitis PigmentosaSequence Homology, Amino AcidSwineSyndromeTissue DistributionConceptsHair cellsMyosin VIIa expressionEpithelial cellsMyosin VIIaCochlear hair cellsMyosin VIIa functionOuter hair cellsUsher syndrome type 1BMyosin VIIA geneVestibular dysfunctionCell-specific localizationCongenital deafnessRetinitis pigmentosaUsher syndromeType 1bType 1B.Normal functionApical stereociliaVIIaDeafnessDisease phenotypeCochleaInherited diseaseRetinaShaker-1
1994
Cell coupling in the supporting cells of Corti's organ: Sensitivity to intracellular H+ and Ca+2
Sato Y, Santos-Sacchi J. Cell coupling in the supporting cells of Corti's organ: Sensitivity to intracellular H+ and Ca+2. Hearing Research 1994, 80: 21-24. PMID: 7852199, DOI: 10.1016/0378-5955(94)90004-3.Peer-Reviewed Original Research
1985
A Ferritin-containing Cell Type in the Stria Vascularis of the Mouse Inner Ear
Santos-Sacchi J, Marovitz W. A Ferritin-containing Cell Type in the Stria Vascularis of the Mouse Inner Ear. Acta Oto-Laryngologica 1985, 100: 26-32. PMID: 4024891, DOI: 10.3109/00016488509108583.Peer-Reviewed Original Research
1982
Intracellular Recordings from Cochlear Outer Hair Cells
Dallos P, Santos-Sacchi J, Flock Å. Intracellular Recordings from Cochlear Outer Hair Cells. Science 1982, 218: 582-584. PMID: 7123260, DOI: 10.1126/science.7123260.Peer-Reviewed Original ResearchAn electronmicroscopic study of microtubules in the development of marginal cells of the mouse stria vascularis
Santos-Sacchi J. An electronmicroscopic study of microtubules in the development of marginal cells of the mouse stria vascularis. Hearing Research 1982, 6: 7-13. PMID: 7054137, DOI: 10.1016/0378-5955(82)90003-x.Peer-Reviewed Original ResearchConceptsMouse stria vascularisPlasma membraneCellular extensionsNumerous cellular processesMarginal cellsSheet-like extensionsCellular processesCell peripheryLength of microtubulesGolgi bodiesDepolymerization of microtubulesCytoplasmic microtubulesMicrotubulesNumerous microtubulesCell processesOrganellesMitochondriaStria vascularisCellsMembraneAdult miceCytoplasmVesiclesPlasmalemmaMicrotubles
1980
An Evaluation of Normal Strial Capillary Transport Using the Electron-Opaque Tracers Ferritin and Iron Dextran
Santos-Sacchi J, Marovitz W. An Evaluation of Normal Strial Capillary Transport Using the Electron-Opaque Tracers Ferritin and Iron Dextran. Acta Oto-Laryngologica 1980, 89: 12-26. PMID: 7405570, DOI: 10.3109/00016488009127103.Peer-Reviewed Original Research