2022
Single particle cryo-EM structure of the outer hair cell motor protein prestin
Butan C, Song Q, Bai JP, Tan WJT, Navaratnam D, Santos-Sacchi J. Single particle cryo-EM structure of the outer hair cell motor protein prestin. Nature Communications 2022, 13: 290. PMID: 35022426, PMCID: PMC8755724, DOI: 10.1038/s41467-021-27915-z.Peer-Reviewed Original ResearchConceptsTransmembrane domainProtein prestinSingle-particle cryo-EM structuresAnti-sigma factor antagonist domainOuter hair cell motor protein prestinCryo-EM structureCryo-electron microscopyMotor protein prestinSLC26 family membersSulfate transportersTransmembrane segmentsPrestin functionÅ resolutionPrestinOHC electromotilityOpen stateCochlear amplificationPutative mechanismsFamily membersDomainRepeatsSLC26A9TransportersMutationsElectromotility
2019
Prestin kinetics and corresponding frequency dependence augment during early development of the outer hair cell within the mouse organ of Corti
Bai JP, Navaratnam D, Santos-Sacchi J. Prestin kinetics and corresponding frequency dependence augment during early development of the outer hair cell within the mouse organ of Corti. Scientific Reports 2019, 9: 16460. PMID: 31712635, PMCID: PMC6848539, DOI: 10.1038/s41598-019-52965-1.Peer-Reviewed Original ResearchConceptsOuter hair cellsPostnatal dayVoltage-sensor charge movementAltricial animalsPostnatal developmentCochlear placeElectrical correlatesHair cellsMouse organsCochlear amplificationEarly developmentAuditory frequencyPrestin activityAuditory capabilitiesCellsCharge movementHigh frequencyResponse increasesPrestin functionMiceActivityMonthsCourse
2018
The Frequency Response of Outer Hair Cell Voltage-Dependent Motility Is Limited by Kinetics of Prestin
Santos-Sacchi J, Tan W. The Frequency Response of Outer Hair Cell Voltage-Dependent Motility Is Limited by Kinetics of Prestin. Journal Of Neuroscience 2018, 38: 5495-5506. PMID: 29899032, PMCID: PMC6001036, DOI: 10.1523/jneurosci.0425-18.2018.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAuditory PerceptionFemaleGuinea PigsHair Cells, Auditory, OuterKineticsMaleMechanotransduction, CellularMiceMolecular Motor ProteinsProteinsConceptsFrequency responseVoltage excitationNonlinear capacitanceFlat frequency responseLow-pass behaviorInterrogation frequencyMechanical responsePiezoelectric couplingCutoff frequencyLow-pass characteristicsLow passElectrical signatureLimiting timeOrders of magnitudeAmplification gainCochlear amplificationKHzΜsMembrane protein prestinVoltageKineticsCapacitanceDamage resultsPhase correspondsElectrical
2013
Disparities in voltage-sensor charge and electromotility imply slow chloride-driven state transitions in the solute carrier SLC26a5
Song L, Santos-Sacchi J. Disparities in voltage-sensor charge and electromotility imply slow chloride-driven state transitions in the solute carrier SLC26a5. Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 110: 3883-3888. PMID: 23431177, PMCID: PMC3593883, DOI: 10.1073/pnas.1218341110.Peer-Reviewed Original Research
2010
A highly expressing Tet-inducible cell line recapitulates in situ developmental changes in prestin's Boltzmann characteristics and reveals early maturational events
Bian S, Koo BW, Kelleher S, Santos-Sacchi J, Navaratnam DS. A highly expressing Tet-inducible cell line recapitulates in situ developmental changes in prestin's Boltzmann characteristics and reveals early maturational events. American Journal Of Physiology - Cell Physiology 2010, 299: c828-c835. PMID: 20631244, PMCID: PMC3774197, DOI: 10.1152/ajpcell.00182.2010.Peer-Reviewed Original Research
2008
Anion Control of Voltage Sensing by the Motor Protein Prestin in Outer Hair Cells
Rybalchenko V, Santos-Sacchi J. Anion Control of Voltage Sensing by the Motor Protein Prestin in Outer Hair Cells. Biophysical Journal 2008, 95: 4439-4447. PMID: 18658219, PMCID: PMC2567960, DOI: 10.1529/biophysj.108.134197.Peer-Reviewed Original ResearchMeSH KeywordsAlkanesulfonatesAnimalsBinding, CompetitiveChlorineGuinea PigsHair Cells, Auditory, OuterIntracellular SpaceMembrane PotentialsMolecular Motor ProteinsProteinsConceptsOuter hair cellsHair cellsCochlear amplificationSLC26 anion transporter familyMotor protein prestinMammalian cochlear amplificationVoltage-sensor charge movementAuditory responsivenessCorti's organAnion transporter familyTransporter familyProtein prestinMolecular motorsCharge movementPrestinOrgansCellsComplicated roleVoltage sensingCochlear Mechanics: No Shout but a Twist in the Absence of Prestin
Santos-Sacchi J. Cochlear Mechanics: No Shout but a Twist in the Absence of Prestin. Current Biology 2008, 18: r304-r306. PMID: 18397741, DOI: 10.1016/j.cub.2008.02.019.Peer-Reviewed Original Research
2007
Developmental Expression of the Outer Hair Cell Motor Prestin in the Mouse
Abe T, Kakehata S, Kitani R, Maruya S, Navaratnam D, Santos-Sacchi J, Shinkawa H. Developmental Expression of the Outer Hair Cell Motor Prestin in the Mouse. The Journal Of Membrane Biology 2007, 215: 49-56. PMID: 17415610, PMCID: PMC4154540, DOI: 10.1007/s00232-007-9004-5.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsGene Expression Regulation, DevelopmentalHair Cells, Auditory, OuterMiceMice, Inbred C57BLMolecular Motor ProteinsProtein ConformationProteinsConceptsLateral membranesOuter hair cellsMouse outer hair cellsMotor protein activitySteady-state levelsKnockout experimentsProtein activityDevelopmental expressionMembrane tensionGenetic modificationConformational fluctuationsCellular mechanismsMotor moleculesMolecular motorsOnset of hearingMorphological estimatesProtein densityEarly maturationQuantitative polymerase chain reactionHair cellsVpkCmDevelopmental dataCochlear amplificationMaturationFull maturation
2006
En block C-terminal charge cluster reversals in prestin (SLC26A5): Effects on voltage-dependent electromechanical activity
Bai JP, Navaratnam D, Samaranayake H, Santos-Sacchi J. En block C-terminal charge cluster reversals in prestin (SLC26A5): Effects on voltage-dependent electromechanical activity. Neuroscience Letters 2006, 404: 270-275. PMID: 16839688, DOI: 10.1016/j.neulet.2006.05.062.Peer-Reviewed Original Research
2005
N-Terminal-Mediated Homomultimerization of Prestin, the Outer Hair Cell Motor Protein
Navaratnam D, Bai JP, Samaranayake H, Santos-Sacchi J. N-Terminal-Mediated Homomultimerization of Prestin, the Outer Hair Cell Motor Protein. Biophysical Journal 2005, 89: 3345-3352. PMID: 16113116, PMCID: PMC1366831, DOI: 10.1529/biophysj.105.068759.Peer-Reviewed Original ResearchMeSH KeywordsAllosteric SiteAmino Acid SequenceAnimalsCell MembraneCell SeparationCHO CellsCricetinaeDimerizationElectric CapacitanceElectric ConductivityElectrophysiologyFlow CytometryFluorescence Resonance Energy TransferGerbillinaeGreen Fluorescent ProteinsHair Cells, Auditory, OuterMechanotransduction, CellularMembrane PotentialsMicroscopy, ConfocalMicroscopy, FluorescenceModels, BiologicalMolecular Motor ProteinsMolecular Sequence DataMutationProtein BindingProtein ConformationProtein Structure, TertiaryProteinsTransfectionConceptsOuter Hair Cell Motor ProteinStructure-function relationsNormal membrane targetingMammalian cochlear amplificationIntact N-terminusMembrane targetingDomain proteinsMembrane topologyFluorescence resonance energy transferCell mechanical responsePrestin moleculesMembrane motorMotor proteinsN-terminusShort truncationsPrestinResonance energy transferIntracellular terminiAllosteric modulationProteinTerminusCochlear amplificationVoltage sensingHomomultimerizationModified activity
2004
On Membrane Motor Activity and Chloride Flux in the Outer Hair Cell: Lessons Learned from the Environmental Toxin Tributyltin
Song L, Seeger A, Santos-Sacchi J. On Membrane Motor Activity and Chloride Flux in the Outer Hair Cell: Lessons Learned from the Environmental Toxin Tributyltin. Biophysical Journal 2004, 88: 2350-2362. PMID: 15596517, PMCID: PMC1305283, DOI: 10.1529/biophysj.104.053579.Peer-Reviewed Original ResearchConceptsLateral membranesCell’s mechanical activityOuter hair cellsMammalian cochlear amplifierHair cellsMembrane motorMarine mammalsIntact outer hair cellsPotent abilityFood chainNonselective conductanceCl(-) homeostasisPrestinNanomolar concentrationsExtracellular Cl- concentrationCl fluxMembraneCellsNew environmental threatsMammalsChloride effectTributyltinEnvironmental threatsHomeostasisNonlinear capacitance
2003
Excitation of fluorescent dyes inactivates the outer hair cell integral membrane motor protein prestin and betrays its lateral mobility
Santos-Sacchi J, Zhao HB. Excitation of fluorescent dyes inactivates the outer hair cell integral membrane motor protein prestin and betrays its lateral mobility. Pflügers Archiv - European Journal Of Physiology 2003, 446: 617-622. PMID: 12783229, DOI: 10.1007/s00424-003-1053-8.Peer-Reviewed Original Research
2001
Temperature dependence of non-linear capacitance in human embryonic kidney cells transfected with prestin, the outer hair cell motor protein
Meltzer J, Santos-Sacchi J. Temperature dependence of non-linear capacitance in human embryonic kidney cells transfected with prestin, the outer hair cell motor protein. Neuroscience Letters 2001, 313: 141-144. PMID: 11682147, DOI: 10.1016/s0304-3940(01)02266-2.Peer-Reviewed Original Research