2022
Typhoid toxin sorting and exocytic transport from Salmonella Typhi-infected cells
Chang SJ, Hsu YT, Chen Y, Lin YY, Lara-Tejero M, Galan JE. Typhoid toxin sorting and exocytic transport from Salmonella Typhi-infected cells. ELife 2022, 11: e78561. PMID: 35579416, PMCID: PMC9142146, DOI: 10.7554/elife.78561.Peer-Reviewed Original ResearchConceptsCellular machineryType III protein secretion systemSpecific cellular machineryVesicle carriersProtein secretion systemExtracellular spaceTyphoid toxinEssential virulence factorExocytic transportGTPase Sar1Syntaxin 4Unusual biologySecretion systemPlasma membraneIntracellular transportRemarkable adaptationSpecific effectorsHost cellsIntracellular pathogensVirulence factorsMachineryCooptionVacuolesToxinSpecific environment
2008
Interaction of Campylobacter jejuni with Host Cells
Watson R, Galán J. Interaction of Campylobacter jejuni with Host Cells. 2008, 287-296. DOI: 10.1128/9781555815554.ch16.Peer-Reviewed Original ResearchHost cellsBacterial internalizationCell biologyHost cell gene expressionVesicular trafficking pathwaysPowerful genetic toolsCell gene expressionEndocytic machineryTrafficking pathwaysStrong phenotypeGenetic toolsCampylobacter jejuniJejuni mutantsGene expressionIntestinal epithelial cellsMicrobial pathogensInnate immune responseNonphagocytic cellsOwn uptakeBacterial determinantsSpecific adaptationsIntracellular pathogensMutantsPathogenic bacteriaCell interactionsModulation of the actin cytoskeleton by Salmonella
Galan J. Modulation of the actin cytoskeleton by Salmonella. The FASEB Journal 2008, 22: 530.1-530.1. DOI: 10.1096/fasebj.22.1_supplement.530.1.Peer-Reviewed Original ResearchEffector proteinsHost cellsActin cytoskeletonBacterial proteinsCellular responsesBacterial effector proteinsProtein secretion systemSpecialized protein secretion systemActin cytoskeletal reorganizationDownstream effector proteinsSignal transduction pathwaysProtein Cdc42Exchange factorMembrane rufflingSmall GTPRho GTPasesSecretion systemCytoskeletal reorganizationTransduction pathwaysCytoskeletal rearrangementsCdc42RhoG.ProteinCytoskeletonRac
2006
Differential activation and function of Rho GTPases during Salmonella–host cell interactions
Patel JC, Galán J. Differential activation and function of Rho GTPases during Salmonella–host cell interactions. Journal Of Cell Biology 2006, 175: 453-463. PMID: 17074883, PMCID: PMC2064522, DOI: 10.1083/jcb.200605144.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBacterial ProteinsCdc42 GTP-Binding ProteinCell MembraneChlorocebus aethiopsCOS CellsEnzyme ActivationGuanine Nucleotide Exchange FactorsHumansIntestinal MucosaMutationRac1 GTP-Binding ProteinRho GTP-Binding ProteinsRNA InterferenceSalmonella InfectionsSalmonella typhimuriumTransfectionConceptsRho family GTPasesExchange factorCellular responsesRho family guanosine triphosphatasesSalmonella-host cell interactionsType III secretion systemSpecific Rho family GTPasesActin cytoskeleton remodelingDifferent Rho family GTPasesSpecific cellular responsesActin remodelingGuanosine triphosphatasesRho GTPasesSecretion systemCytoskeleton remodelingBacterial proteinsGTPasesSophisticated mechanismsHost cellsDistinct rolesBacterial pathogensCell interactionsSalmonella entericaDifferential activationCentral role
2004
Salmonella Modulates Vesicular Traffic by Altering Phosphoinositide Metabolism
Hernandez LD, Hueffer K, Wenk MR, Galán J. Salmonella Modulates Vesicular Traffic by Altering Phosphoinositide Metabolism. Science 2004, 304: 1805-1807. PMID: 15205533, DOI: 10.1126/science.1098188.Peer-Reviewed Original ResearchConceptsIntracellular replicative nicheType III secretion systemActin cytoskeleton rearrangementBacteria-containing vacuolesBacterial intracellular growthPhosphoinositide phosphataseInnate immune defenseSecretion systemReplicative nicheBacterial entryCytoskeleton rearrangementSpacious phagosomesHost cellsNonphagocytic cellsIntracellular growthImmune defenseSopBPhosphoinositide metabolismSignificant defectsSalmonella entericaVacuolesMembraneCellsNichePhagosomes
2003
A Salmonella protein causes macrophage cell death by inducing autophagy
Hernandez LD, Pypaert M, Flavell RA, Galán J. A Salmonella protein causes macrophage cell death by inducing autophagy. Journal Of Cell Biology 2003, 163: 1123-1131. PMID: 14662750, PMCID: PMC2173598, DOI: 10.1083/jcb.200309161.Peer-Reviewed Original ResearchConceptsMembrane fusion activityCell deathType III protein secretion systemFusion activityProtein secretion systemMacrophage cell deathEndoplasmic reticulum markerSalmonella-infected macrophagesSecretion systemSalmonella proteinsAutophagic vesiclesMutant formsSipBHost cellsCultured cellsMitochondriaMembrane structureSalmonella entericaProteinCausative agentMarked accumulationMacrophagesCellsAutophagosomesAutophagy
2001
Role of tyrosine kinases and the tyrosine phosphatase SptP in the interaction of Salmonella with host cells
Murli S, Watson R, Galán J. Role of tyrosine kinases and the tyrosine phosphatase SptP in the interaction of Salmonella with host cells. Cellular Microbiology 2001, 3: 795-810. PMID: 11736992, DOI: 10.1046/j.1462-5822.2001.00158.x.Peer-Reviewed Original ResearchMeSH KeywordsActinsBacterial ProteinsCdc42 GTP-Binding ProteinCell NucleusCytoskeletonInterleukin-8Mitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesPhosphorylationPhosphotyrosineProtein Tyrosine PhosphatasesProtein-Tyrosine KinasesSalmonellaSalmonella typhimuriumSignal TransductionSubstrate SpecificityTransfectionTyrosineVimentinConceptsTyrosine phosphatase activityBacterial proteinsCellular responsesPhosphatase activityTyrosine kinaseHost cellsType III secretion systemIntermediate filament protein vimentinCarboxy-terminal domainRho family GTPases Cdc42Actin cytoskeleton reorganizationCellular transcription factorsMAP kinase activationInteraction of SalmonellaMembrane rufflesGAP activitySpecialized organellesGTPases Cdc42Secretion systemTranscription factorsCytoskeleton reorganizationKinase activationProtein vimentinBacterial uptakeProteinSALMONELLA INTERACTIONS WITH HOST CELLS: Type III Secretion at Work
Galán J. SALMONELLA INTERACTIONS WITH HOST CELLS: Type III Secretion at Work. Annual Review Of Cell And Developmental Biology 2001, 17: 53-86. PMID: 11687484, DOI: 10.1146/annurev.cellbio.17.1.53.Peer-Reviewed Original ResearchConceptsBacterial proteinsBacterial pathogen Salmonella entericaHost cellsType III secretion systemActin cytoskeleton dynamicsType III secretionHost cell functionsPathogen Salmonella entericaMolecular tinkeringConvergent evolutionEndocytic traffickingCytoskeleton dynamicsSpecialized organellesCellular functionsSecretion systemMicrobial pathogenesisRemarkable pathogenHost proteinsSalmonella interactionsVertebrate hostsProteinRemarkable arrayCoordinated actionCell functionSalmonella entericaSalmonella entry into host cells: the work in concert of type III secreted effector proteins
Zhou D, Galán J. Salmonella entry into host cells: the work in concert of type III secreted effector proteins. Microbes And Infection 2001, 3: 1293-1298. PMID: 11755417, DOI: 10.1016/s1286-4579(01)01489-7.Peer-Reviewed Original ResearchConceptsActin cytoskeleton rearrangementCytoskeleton rearrangementActin dynamicsHost cellsHost actin dynamicsHost signal transductionType III secretion systemActin-binding proteinsSPI-1 type III secretion systemEffector proteinsSecretion systemSignal transductionActin rearrangementBacterial proteinsSalmonella entryIntestinal epithelial cellsBacterial uptakeCdc42Coordinated stepsProteinEpithelial cellsRacRearrangementCellsTransductionA Salmonella inositol polyphosphatase acts in conjunction with other bacterial effectors to promote host cell actin cytoskeleton rearrangements and bacterial internalization
Zhou D, Chen L, Hernandez L, Shears S, Galán J. A Salmonella inositol polyphosphatase acts in conjunction with other bacterial effectors to promote host cell actin cytoskeleton rearrangements and bacterial internalization. Molecular Microbiology 2001, 39: 248-260. PMID: 11136447, DOI: 10.1046/j.1365-2958.2001.02230.x.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBacterial ProteinsCdc42 GTP-Binding ProteinCell MembraneCells, CulturedChlorocebus aethiopsCOS CellsCytoskeletonHumansInositol PhosphatesIntestinesJNK Mitogen-Activated Protein KinasesMAP Kinase Kinase 4Mitogen-Activated Protein Kinase KinasesPhosphoric Monoester HydrolasesPhosphorylationSalmonella InfectionsSalmonella typhimuriumTransfectionConceptsActin cytoskeleton rearrangementCytoskeleton rearrangementBacterial entrySecretion systemBacterial internalizationCellular responsesHost cellsRho GTPases signalingProtein secretion systemHost cell actin cytoskeleton rearrangementsHost cellular functionsSpecialized protein secretion systemCdc42-dependent mannerNon-phagocytic cellsBacterial effectorsAbility of SalmonellaInositol phosphataseCellular functionsDefective mutantsBacterial proteinsCo-ordinated functionSalmonella pathogenicityBacterium's abilitySopBPhospholipase C.
2000
Biophysical characterization of SipA, an actin‐binding protein from Salmonella enterica
Mitra K, Zhou D, Galán J. Biophysical characterization of SipA, an actin‐binding protein from Salmonella enterica. FEBS Letters 2000, 482: 81-84. PMID: 11018527, DOI: 10.1016/s0014-5793(00)02040-8.Peer-Reviewed Original ResearchConceptsActin dynamicsCoiled-coil domainF-actin stabilityActin-binding proteinsActin cytoskeleton reorganizationNon-phagocytic cellsBacterial entryCytoskeleton reorganizationAdjacent actin monomersC-terminal fragmentBiophysical characterizationF-actinCellular responsesHost cellsBacterial uptakeActin monomersProteinSalmonella entericaHelical conformationSalmonella enterica infectionsIntestinal epitheliumEnterica infectionsEssential stepSIPACellsMolecular characterization and assembly of the needle complex of the Salmonella typhimurium type III protein secretion system
Kubori T, Sukhan A, Aizawa S, Galán J. Molecular characterization and assembly of the needle complex of the Salmonella typhimurium type III protein secretion system. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 10225-10230. PMID: 10944190, PMCID: PMC27824, DOI: 10.1073/pnas.170128997.Peer-Reviewed Original ResearchConceptsType III secretion systemProtein secretion systemSecretion systemNeedle complexType III protein secretion systemHost cellular functionsSpecialized protein secretion systemType III secretion export apparatusType III secretionPathogenicity island 1Secretion complexPathogen's benefitExport apparatusCellular functionsAnimal cellsNeedle-like projectionsBacterial proteinsBacterium's abilityNeedle substructureIsland 1Host cellsMolecular characterizationNonphagocytic cellsNeedle componentBacterial pathogensStriking a balance: Modulation of the actin cytoskeleton by Salmonella
Galán J, Zhou D. Striking a balance: Modulation of the actin cytoskeleton by Salmonella. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 8754-8761. PMID: 10922031, PMCID: PMC34008, DOI: 10.1073/pnas.97.16.8754.Peer-Reviewed Original ResearchConceptsType III secretion systemSecretion systemHost cellsBacterial entryGTP-binding protein Cdc42Bacterial effector proteinsInositol polyphosphate phosphatasePathogen/host relationshipActin cytoskeleton rearrangementHost cell cytosolStimulation of Cdc42Protein Cdc42Effector proteinsActin cytoskeletonExchange factorSpecialized organellesSalmonella proteinsCytoskeleton rearrangementT-plastinHost relationshipsCell cytosolF-actinCellular responsesG proteinsCdc42
1999
An invasion-associated Salmonella protein modulates the actin-bundling activity of plastin
Zhou D, Mooseker M, Galán J. An invasion-associated Salmonella protein modulates the actin-bundling activity of plastin. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 10176-10181. PMID: 10468582, PMCID: PMC17862, DOI: 10.1073/pnas.96.18.10176.Peer-Reviewed Original ResearchConceptsActin-bundling activityT-plastinBacterial-host cell contactHost cell signal transduction pathwaysHost cellsType III secretion systemBacterial effector proteinsActin-binding proteinsSignal transduction pathwaysBacterial effectorsMembrane rufflesEffector proteinsActin cytoskeletonMembrane rufflingSecretion systemSalmonella proteinsBacterial entryBacterial internalizationSalmonella entrySignaling processesActin filamentsF-actinNonphagocytic cellsProteinCell contactDifferential Regulation of Salmonella typhimurium Type III Secreted Proteins by Pathogenicity Island 1 (SPI-1)-Encoded Transcriptional Activators InvF and HilA
Eichelberg K, Galán J. Differential Regulation of Salmonella typhimurium Type III Secreted Proteins by Pathogenicity Island 1 (SPI-1)-Encoded Transcriptional Activators InvF and HilA. Infection And Immunity 1999, 67: 4099-4105. PMID: 10417179, PMCID: PMC96710, DOI: 10.1128/iai.67.8.4099-4105.1999.Peer-Reviewed Original ResearchConceptsExpression of componentsSPI-1Secretion systemRegulatory proteinsType III protein secretion systemType III secretion machineryCellular responsesHost cellsFunction mutationsType III secretion systemInvasion-associated type III secretion systemProtein secretion systemTranscriptional regulatory proteinsComplex regulatory mechanismsPathogenicity island 1Secretion machineryCentisome 63Different genesRegulatory mechanismsMutant strainPathogenicity islandInvFIsland 1Differential regulationProteinCharacterization of SprA, an AraC‐like transcriptional regulator encoded within the Salmonella typhimurium pathogenicity island 1
Eichelberg K, Hardt W, Galán J. Characterization of SprA, an AraC‐like transcriptional regulator encoded within the Salmonella typhimurium pathogenicity island 1. Molecular Microbiology 1999, 33: 139-152. PMID: 10411731, DOI: 10.1046/j.1365-2958.1999.01458.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAraC Transcription FactorBacterial ProteinsBiological TransportCells, CulturedChromosome MappingChromosomes, BacterialConsensus SequenceDNA-Binding ProteinsEpithelial CellsGene Expression Regulation, BacterialGenes, BacterialGenes, ReporterHelix-Loop-Helix MotifsMolecular Sequence DataMultigene FamilyPhylogenyRecombinant Fusion ProteinsRepressor ProteinsSalmonella typhimuriumSequence AlignmentSequence Homology, Amino AcidSignal TransductionTrans-ActivatorsTranscription FactorsTranscription, GeneticVirulenceConceptsType III secretion systemTranscriptional regulatory proteinsPathogenicity island 1Secretion systemRegulatory proteinsType III protein secretion systemSPI-1Island 1Host cellsProtein secretion systemTranscriptional regulatory cascadeSpecific transcriptional regulatory proteinsAdditional regulatory proteinsSignificant sequence similarityAraC-like transcriptional regulatorActin cytoskeletal rearrangementRegulation of expressionInvasion-associated genesExpression of genesNon-phagocytic cellsCentisome 63Effector proteinsTranscriptional regulatorsRegulatory cascadeSequence similarityType III Secretion Machines: Bacterial Devices for Protein Delivery into Host Cells
Galán J, Collmer A. Type III Secretion Machines: Bacterial Devices for Protein Delivery into Host Cells. Science 1999, 284: 1322-1328. PMID: 10334981, DOI: 10.1126/science.284.5418.1322.Peer-Reviewed Original ResearchConceptsSecretion systemComplex protein secretion systemsHost cellsType III secretion systemBacterial effector proteinsProtein secretion systemAnimal pathogenic bacteriaHost cellular functionsGram-negative pathogenic bacteriaBacterial devicesPathogenic bacteriaEffector proteinsCellular functionsFlagellar apparatusBacterial speciesEffector moleculesProtein deliveryBacteriaCellsNovel preventionBiologySpeciesPlantsProteinTherapeutic approachesRole of the S. typhimurium Actin-Binding Protein SipA in Bacterial Internalization
Zhou D, Mooseker M, Galán J. Role of the S. typhimurium Actin-Binding Protein SipA in Bacterial Internalization. Science 1999, 283: 2092-2095. PMID: 10092234, DOI: 10.1126/science.283.5410.2092.Peer-Reviewed Original ResearchInteraction of Salmonella spp. with host cells
Murli S, Galán J. Interaction of Salmonella spp. with host cells. Advances In Cellular And Molecular Biology Of Membranes And Organelles 1999, 6: 173-180. DOI: 10.1016/s1874-5172(99)80011-x.Peer-Reviewed Original ResearchEffector proteinsHost cellsBacterial effector proteinsHost cell cytosolSignal transduction pathwaysHost cell responsesFacultative intracellular pathogenActin cytoskeletonMembrane rufflingTranscription factorsTransduction pathwaysElaborate mechanismsCell cytosolSubsequent translocationMicrobial pathogensCell deathHost environmentCell typesIntracellular pathogensDistinct responsesSalmonella entericaPathogensHostProteinIntimate interaction
1998
Identification of a Specific Chaperone for SptP, a Substrate of the Centisome 63 Type III Secretion System ofSalmonella typhimurium
Fu Y, Galán J. Identification of a Specific Chaperone for SptP, a Substrate of the Centisome 63 Type III Secretion System ofSalmonella typhimurium. Journal Of Bacteriology 1998, 180: 3393-3399. PMID: 9642193, PMCID: PMC107295, DOI: 10.1128/jb.180.13.3393-3399.1998.Peer-Reviewed Original ResearchMeSH KeywordsActinsAmino Acid SequenceBacterial ProteinsBase SequenceChromosomes, BacterialCloning, MolecularComplement Inactivator ProteinsCytoskeletonKineticsMolecular Sequence DataPlasmidsPolymerase Chain ReactionProtein Tyrosine PhosphatasesRecombinant Fusion ProteinsSalmonella typhimuriumSequence AlignmentSequence Homology, Amino AcidConceptsType III secretion systemSecretion systemSpecific chaperonesActin cytoskeletonTyrosine phosphataseType III protein secretion systemHost cell actin cytoskeletonHost cellsProtein secretion systemCell actin cytoskeletonHost cell responsesPulse-chase experimentsCytoplasmic chaperonesCentisome 63Effector proteinsTranscription factorsAcidic polypeptidesChaperonesResidues 15Function mutationsEffector moleculesCytoskeletonProteinSPTPSalmonella typhimurium