Featured Publications
Three-dimensional adaptive optical nanoscopy for thick specimen imaging at sub-50-nm resolution
Hao X, Allgeyer ES, Lee DR, Antonello J, Watters K, Gerdes JA, Schroeder LK, Bottanelli F, Zhao J, Kidd P, Lessard MD, Rothman JE, Cooley L, Biederer T, Booth MJ, Bewersdorf J. Three-dimensional adaptive optical nanoscopy for thick specimen imaging at sub-50-nm resolution. Nature Methods 2021, 18: 688-693. PMID: 34059828, PMCID: PMC7610943, DOI: 10.1038/s41592-021-01149-9.Peer-Reviewed Original ResearchMeSH KeywordsImaging, Three-DimensionalMicroscopy, FluorescenceNanotechnologyOptics and PhotonicsSignal-To-Noise RatioConceptsAdaptive optics schemeBest possible spatial resolutionPossible spatial resolutionOptical nanoscopyOptics schemeDiffraction limitDark stateOptical aberrationsNanoscopy methodsThin samplesThick specimenFluorescent moleculesSpatial resolutionThick specimensNanoscopeIsotropic resolutionResolutionNanoscopyPath geometryFluorescence microscopyMicroscopeMicroscopy
2023
Extracting nanoscale membrane morphology from single-molecule localizations
Marin Z, Fuentes L, Bewersdorf J, Baddeley D. Extracting nanoscale membrane morphology from single-molecule localizations. Biophysical Journal 2023, 122: 3022-3030. PMID: 37355772, PMCID: PMC10432223, DOI: 10.1016/j.bpj.2023.06.010.Peer-Reviewed Original Research
2017
Biological Insight from Super-Resolution Microscopy: What We Can Learn from Localization-Based Images
Baddeley D, Bewersdorf J. Biological Insight from Super-Resolution Microscopy: What We Can Learn from Localization-Based Images. Annual Review Of Biochemistry 2017, 87: 1-25. PMID: 29272143, DOI: 10.1146/annurev-biochem-060815-014801.Peer-Reviewed Original Research
2016
Two-colour live-cell nanoscale imaging of intracellular targets
Bottanelli F, Kromann EB, Allgeyer ES, Erdmann RS, Wood Baguley S, Sirinakis G, Schepartz A, Baddeley D, Toomre DK, Rothman JE, Bewersdorf J. Two-colour live-cell nanoscale imaging of intracellular targets. Nature Communications 2016, 7: 10778. PMID: 26940217, PMCID: PMC4785223, DOI: 10.1038/ncomms10778.Peer-Reviewed Original Research
2013
Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithms
Huang F, Hartwich TM, Rivera-Molina FE, Lin Y, Duim WC, Long JJ, Uchil PD, Myers JR, Baird MA, Mothes W, Davidson MW, Toomre D, Bewersdorf J. Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithms. Nature Methods 2013, 10: 653-658. PMID: 23708387, PMCID: PMC3696415, DOI: 10.1038/nmeth.2488.Peer-Reviewed Original Research